Comparative Investigations of the Mechanical Characteristics of the Material of Endotracheal Tubes

Коледенков, А. А.; Zyukin, S.V.; Dyatlov, V. A.

doi:10.1177/0307174x1304000902

Cited by 1 publication

(1 citation statement)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The elastic moduli of PVC, polystyrene and borosilicate glass are 14.5, 3.3 and 64 GPa, respectively [107][108][109]. This suggests that PVC is likely the most elastic material (363.6 % of elongation before yield) followed by polystyrene (1.5-3 % of elongation before yield) and glass is least elastic [108,112]. The hyper-and hypobiofilm formation on polystyrene and ETT, respectively, by PA14gltR indicates that the GltR response regulator may respond to surfaces with low stiffness.…”

Section: Discussionmentioning

confidence: 99%

Pseudomonas aeruginosa biofilm formation on endotracheal tubes requires multiple two-component systems

Badal

Jayarani

Kollaran

et al. 2020

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Indwelling medical devices such as endotracheal tubes (ETTs), urinary catheters, vascular access devices, tracheostomies and feeding tubes are often associated with hospital-acquired infections. Bacterial biofilm formed on the ETTs in intubated patients is a significant risk factor associated with ventilator-associated pneumonia. Pseudomonas aeruginosa is one of the four frequently encountered bacteria responsible for causing pneumonia, and the biofilm formation on ETTs. However, understanding of biofilm formation on ETT and interventions to prevent biofilm remains lagging. The ability to sense and adapt to external cues contributes to their success. Thus, the biofilm formation is likely to be influenced by the two-component systems (TCSs) that are composed of a membrane-associated sensor kinase and an intracellular response regulator. Aim. This study aims to establish an in vitro method to analyse the P. aeruginosa biofilm formation on ETTs, and identify the TCSs that contribute to this process. Methodology. In total, 112 P. aeruginosa PA14 TCS mutants were tested for their ability to form biofilm on ETTs, their effect on quorum sensing (QS) and motility. Results. Out of 112 TCS mutants studied, 56 had altered biofilm biomass on ETTs. Although the biofilm formation on ETTs is QS-dependent, none of the 56 loci controlled quorum signal. Of these, 18 novel TCSs specific to ETT biofilm were identified, namely, AauS, AgtS, ColR, CopS, CprR, NasT, KdpD, ParS, PmrB, PprA, PvrS, RcsC, PA14_11120, PA14_32580, PA14_45880, PA14_49420, PA14_52240, PA14_70790. The set of 56 included the GacS network, TCS proteins involved in fimbriae synthesis, TCS proteins involved in antimicrobial peptide resistance, and surface-sensing. Additionally, several of the TCS-encoding genes involved in biofilm formation on ETTs were found to be linked to flagellum-dependent swimming motility. Conclusions. Our study established an in vitro method for studying P. aeruginosa biofilm formation on the ETT surfaces. We also identified novel ETT-specific TCSs that could serve as targets to prevent biofilm formation on indwelling devices frequently used in clinical settings.