Comparative investigations of cytotoxic action of anticancer drugs Taxol and Taxotere on meristematic cells of Allium cepa

Majewska, Agnieszka; Śliwińska, Elwira; Wierzbicka, Małgorzata; Kuraś, Mieczysław

doi:10.1080/00087114.2008.10589607

Cited by 5 publications

(1 citation statement)

References 36 publications

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“…Paclitaxel (Sigma) was used as a positive control at concentrations ranging from 0.00075 to 10 μM. Paclitaxel was incubated with cells for either 24 or 48 h. , Peptides and paclitaxel were tested in triplicate, and the data were analyzed using the statistical software package GraphPad Prism to obtain IC 50 values from the sigmoidal concentration–response curves.…”

Section: Methodsmentioning

confidence: 99%

Discovery and Characterization of Cyclotides from Rinorea Species

et al. 2018

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Cyclotides are macrocyclic cystine-knotted peptides most commonly found in the Violaceae plant family. Although Rinorea is the second-largest genera within the Violaceae family, few studies have examined whether or not they contain cyclotides. To further our understanding of cyclotide diversity and evolution, we examined the cyclotide content of two Rinorea species found in Southeast Asia: R. virgata and R. bengalensis. Seven cyclotides were isolated from R. virgata (named Rivi1-7) and a known cyclotide (cT10) was found in R. bengalensis. Loops 2, 5 and 6 of Rivi1-4 contained sequences not previously seen in corresponding loops of known cyclotides, thereby expanding our understanding of the diversity of cyclotides. In addition, the sequence of loop 2 of Rivi3 and Rivi4 were identical to some related non-cyclic "acyclotides" from the Poaceae plant family. As only acyclotides (not cyclotides) have been reported in monocotyledons thus far, our findings support an evolutionary link between monocotyledon-derived ancestral cyclotide precursors and dicotyledonderived cyclotides. Furthermore, Rivi2 and Rivi3 had comparable cytotoxic activities to the most cytotoxic cyclotide known to date: cycloviolacin O2 from Viola odorata; yet, unlike cycloviolacin O2, they did not show hemolytic activity. Therefore, these cyclotides represent novel scaffolds for use in future anticancer drug design.

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Section: Methodsmentioning

confidence: 99%