Comparative Investigation of Collagen-Based Hybrid 3D Structures for Potential Biomedical Applications

David, Geta; Bargan, Alexandra; Drobotă, Mioara; Bele, Adrian; Roșca, Irina

doi:10.3390/ma14123313

Cited by 4 publications

(2 citation statements)

References 32 publications

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“…Many studies were focused on natural compounds as promising alternatives to antibiotics for increasing the antimicrobial activity or cellular uptake of materials [ 51 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

Temperature Induced Gelation and Antimicrobial Properties of Pluronic F127 Based Systems

Lupu¹,

Roșca²,

Gradinaru

et al. 2023

Polymers

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Different formulations containing Pluronic F127 and polysaccharides (chitosan, sodium alginate, gellan gum, and κ-carrageenan) were investigated as potential injectable gels that behave as free-flowing liquid with reduced viscosity at low temperatures and displayed solid-like properties at 37 °C. In addition, ZnO nanoparticles, lysozyme, or curcumin were added for testing the antimicrobial properties of the thermal-sensitive gels. Rheological investigations evidenced small changes in transition temperature and kinetics of gelation at 37 °C in presence of polysaccharides. However, the gel formation is very delayed in the presence of curcumin. The antimicrobial properties of Pluronic F127 gels are very modest even by adding chitosan, lysozyme, or ZnO nanoparticles. A remarkable enhancement of antimicrobial activity was observed in the presence of curcumin. Chitosan addition to Pluronic/curcumin systems improves their viscoelasticity, antimicrobial activity, and stability in time. The balance between viscoelastic and antimicrobial characteristics needs to be considered in the formulation of Pluronic F127 gels suitable for biomedical and pharmaceutical applications.

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“…Many studies were focused on natural compounds as promising alternatives to antibiotics for increasing the antimicrobial activity or cellular uptake of materials [ 51 , 76 ].…”

Section: Discussionmentioning

confidence: 99%

Temperature Induced Gelation and Antimicrobial Properties of Pluronic F127 Based Systems

Lupu¹,

Roșca²,

Gradinaru

et al. 2023

Polymers

Self Cite

View full text Add to dashboard Cite

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“…It is the most abundant protein found in the extracellular matrix of animals, constituting approximately 30% of the protein content in vertebrates. Collagen plays a key role in maintaining the biological and structural integrity of the extracellular matrix [ 124 , 125 ]. This protein serves a dual function, combining structural and functional roles.…”

Section: Hydrogels For Chronic Wound Repairmentioning

confidence: 99%

Progress in the Use of Hydrogels for Antioxidant Delivery in Skin Wounds

Maeso,

Antezana,

Hvozda Arana

et al. 2024

Pharmaceutics

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The skin is the largest organ of the body, and it acts as a protective barrier against external factors. Chronic wounds affect millions of people worldwide and are associated with significant morbidity and reduced quality of life. One of the main factors involved in delayed wound healing is oxidative injury, which is triggered by the overproduction of reactive oxygen species. Oxidative stress has been implicated in the pathogenesis of chronic wounds, where it is known to impair wound healing by causing damage to cellular components, delaying the inflammatory phase of healing, and inhibiting the formation of new blood vessels. Thereby, the treatment of chronic wounds requires a multidisciplinary approach that addresses the underlying causes of the wound, provides optimal wound care, and promotes wound healing. Among the promising approaches to taking care of chronic wounds, antioxidants are gaining interest since they offer multiple benefits related to skin health. Therefore, in this review, we will highlight the latest advances in the use of natural polymers with antioxidants to generate tissue regeneration microenvironments for skin wound healing.

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Development of 3D-Printed Collagen Scaffolds with In-Situ Synthesis of Silver Nanoparticles

et al. 2022

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UV-irradiation method has grown as an alternative approach to in situ synthetize silver nanoparticles (AgNPs) for avoiding the use of toxic reducing agents. In this work, an antimicrobial material by in situ synthesizing AgNPs within 3D-printed collagen-based scaffolds (Col-Ag) was developed. By modifying the concentration of AgNO3 (0.05 and 0.1 M) and UV irradiation time (2 h, 4 h, and 6 h), the morphology and size of the in situ prepared AgNPs could be controlled. As a result, star-like silver particles of around 23 ± 4 μm and spherical AgNPs of 220 ± 42 nm were obtained for Ag 0.05 M, while for Ag 0.1 M cubic particles from 0.3 to 1.0 μm and round silver precipitates of 3.0 ± 0.4 μm were formed in the surface of the scaffolds at different UV irradiation times. However, inside the material AgNPs of 10–28 nm were obtained. The DSC thermal analysis showed that a higher concentration of Ag stabilizes the 3D-printed collagen-based scaffolds, while a longer UV irradiation interval produces a decrease in the denaturation temperature of collagen. The enzymatic degradation assay also revealed that the in situ formed AgNPs act as stabilizing and reinforcement agent which also improve the swelling capacity of collagen-based material. Finally, antimicrobial activity of Col-Ag was studied, showing high bactericidal efficiency against Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria. These results showed that the UV irradiation method was really attractive to modulate the size and shape of in situ synthesized AgNPs to develop antimicrobial 3D-printed collagen scaffolds with different thermal, swelling and degradation properties.

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Comparative Investigation of Collagen-Based Hybrid 3D Structures for Potential Biomedical Applications

Cited by 4 publications

References 32 publications

Temperature Induced Gelation and Antimicrobial Properties of Pluronic F127 Based Systems

Temperature Induced Gelation and Antimicrobial Properties of Pluronic F127 Based Systems

Progress in the Use of Hydrogels for Antioxidant Delivery in Skin Wounds

Development of 3D-Printed Collagen Scaffolds with In-Situ Synthesis of Silver Nanoparticles

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