Comparative investigation of Ashwagandha FMB extract and standardized extract for their antioxidant, anti-inflammatory and immunomodulatory potential

Tamoli, Sanjay; Gokarn, Vijay; Ibrahim, Mohammad; Ahmad, Sayeed

doi:10.54085/ap.2022.11.1.46

Cited by 6 publications

(6 citation statements)

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“…On the other side, problems with iron, homeostasis, or in ammation ultimately result in chronic disease. Additionally, it has been claimed that taking calcium and vitamin D supplements lowers in ammation and bacterial dysbiosis [12].…”

Section: Discussionmentioning

confidence: 99%

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Comparative cytotoxicity, immunomodulatory and anti-inflammatory potential of FMB (Fuzed, Micronized and Bio-activate) and conventional extracts of Glycyrrhiza glabra

Ibrahim¹,

Gokarn²,

Tamoli³

2022

Preprint

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Essential trace elements (ETEs) are important for the human body as like plant phytoconstituents in the products or extracts perform cell functions at biological, chemical and molecular levels. ETEs serve as cofactors for numerous enzymes and as centres for maintaining the structural integrity of proteins and enzymes as well as mediating significant biological processes. Therefore, ETEs are required in our therapeutic regimen to attain maximum efficacy. In the present study, we have successfully replenished the ETEs into a conventional extract of Glycyrrhiza glabra by utilizing an advanced technology called FMB [(fusion (F), micronization (M), bioligation or bioactivate (B)] and investigate their cytotoxicity, immunomodulatory and anti-inflammatory activities. The elemental analysis confirmed that FMB technology significantly replenishes iron, zinc and calcium in the conventional extract. Results of cytotoxicity revealed that FMB extract is safer than conventional extract. Moreover, significant pinocytic activity was observed in FMB extract as compared to conventional extract while similar results were observed in a splenocyte proliferation assay. In the case of anti-inflammatory activity, FMB extract showed better activity in comparison to conventional extract. Conclusively, the current study finds that FMB approaches can be a more effective substitute for conventional extracts by offering them a higher therapeutic value.

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Section: Discussionmentioning

confidence: 99%

“…The conventional extract of Glycyrrhiza glabra was purchased from an authentic vendor and its FMB extract was prepared as per the de ned protocol at Abhinav Healthcare Products Private Limited, Mumbai, Maharashtra, India [12]. Further, FMB extract and conventional extract were utilized for the biological activity.…”

Section: Preparation Of Fmb Extractmentioning

confidence: 99%

Comparative cytotoxicity, immunomodulatory and anti-inflammatory potential of FMB (Fuzed, Micronized and Bio-activate) and conventional extracts of Glycyrrhiza glabra

Ibrahim¹,

Gokarn²,

Tamoli³

2022

Preprint

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“…FMB technology replenished iron, zinc, and calcium, and phytochemical analysis identified withanoside IV and withanolide A. ASH-FMB demonstrated higher antioxidant and anti-inflammatory activities than ASH, significantly stimulated spleen cell proliferation, and showed similar results in pinocytic activation. The study suggests that FMB techniques enhance the therapeutic value of standardized extracts …”

Section: Pharmacological Relevance Of Withania Somniferamentioning

confidence: 92%

“…The study suggests that FMB techniques enhance the therapeutic value of standardized extracts. 162 Several studies in various biological models have indeed been conducted to elaborate on the pharmacokinetic properties of WS. In male Sprague−Dawley rats, the validated UHPLC-MS/MS method was employed to calculate seven constituents instantaneously after oral administration of WS extract (500 mg/kg) (n = 6).…”

Section: Pharmacological Relevance Of Withania Somniferamentioning

confidence: 99%

Recent Advancements in Extraction Techniques of Ashwagandha (Withania somnifera) with Insights on Phytochemicals, Structural Significance, Pharmacology, and Current Trends in Food Applications

Shinde,

Balasubramaniam,

Mulay

et al. 2023

ACS Omega

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Ashwagandha, also known as Withania somnifera (WS), is an ayurvedic botanical plant with numerous applications in dietary supplements and traditional medicines worldwide. Due to the restorative qualities of its roots, WS has potent therapeutic value in traditional Indian (Ayurvedic, Unani, Siddha) and modern medicine recognized as the "Indian ginseng". The presence of phytochemical bioactive compounds such as withanolides, withanosides, alkaloids, flavonoids, and phenolic compounds has an important role in the therapeutic and nutritional properties of WS. Thus, the choice of WS plant part and extraction solvents, with conventional and modern techniques, plays a role in establishing WS as a potential nutraceutical product. WS has recently made its way into food supplements and products, such as baked goods, juices, beverages, sweets, and dairy items. The review aims to cover the key perspectives about WS in terms of plant description, phytochemistry, structural significance, and earlier reported extraction methodologies along with the analytical and pharmacological landscape in the area. It also attempts to iterate the key limitations and further insights into extraction techniques and bioactive standardization with the regulatory framework. It presents a key to the future development of prospective applications in foods such as food supplements or functional foods.

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“…Phytoconstituents extracted in the present day processes of making herbal extracts, omits or eliminates the ETEs that can lead to the lower pharmacological potential of the extract. In the present study, we have successfully implemented a process technology of FMB (fusion (F), micronization (M), and bioligation (B) [9] in conventional extract of G. sylvestre to increase its therapeutic potency. This method is simple and can be used to create extracts and formulations that are ready to use in terms of their phytochemical contents and ETEs, as well as to reach higher pharmacological potentials.…”

Section: Introductionmentioning

confidence: 99%

Fusion, Micronization and Bio-activated (FMB) extract of Gymnema sylvestre for higher antidiabetic and antioxidant effects compared to its conventional standardized extract

Ibrahim¹,

Gokarn²,

Tamoli³

2022

Preprint

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Despite considerable studies, diabetes continues to remain a challenge for its prevention and management with an increasing rate of morbidity and mortality. One of the popular herbal extracts - Gymnema sylvestre has been extensively studied for its anti-diabetic potential. The present study aimed to explore the in vitro antidiabetic and antioxidant effects of Gymnema sylvetsre (Gudmar; GD) and also reveal its drug-likeness, toxicity and action mechanism by utilizing in silico molecular docking and network pharmacology, respectively. The elemental analysis confirmed that FMB technology successfully replenishes iron, zinc and calcium in the conventional extract which is devoid of these essential trace elements. GD extract-FMB (IC50 = 131.90 ± 0.44) are more effective as compared to conventional GD extract in the case of α-amylase (IC50 = 118.23 ± 0.42; IC50 = 109.52 ± 0.34) and α-glucosidase (IC50 = 109.52 ± 0.34), respectively while a similar effect was observed in DPPH-free radical scavenging (IC50 = 129.2 ± 0.98) of GD extract-FMB as compared to GD extract (IC50 = 176.6 ± 0.51). Network pharmacology revealed that key target proteins that are strongly linked to iron, zinc and calcium were strongly involved in glucose metabolism, oxo-inflammatory responses and insulin-related pathways. In addition, in silico molecular docking results of gymnemic acid against α-amylase and α-glucosidase revealed strong biological bindings. Conclusively, based on the finding we can conclude that FMB technique replenish the ETEs in the conventional extract. Also FMB processing is beneficial and provides a innovative solution for the herbal pharmaceutical industry to develop better efficacious extracts or herbal formulations.

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Comparative investigation of Ashwagandha FMB extract and standardized extract for their antioxidant, anti-inflammatory and immunomodulatory potential

Cited by 6 publications

References 0 publications

Comparative cytotoxicity, immunomodulatory and anti-inflammatory potential of FMB (Fuzed, Micronized and Bio-activate) and conventional extracts of Glycyrrhiza glabra

Comparative cytotoxicity, immunomodulatory and anti-inflammatory potential of FMB (Fuzed, Micronized and Bio-activate) and conventional extracts of Glycyrrhiza glabra

Recent Advancements in Extraction Techniques of Ashwagandha (Withania somnifera) with Insights on Phytochemicals, Structural Significance, Pharmacology, and Current Trends in Food Applications

Fusion, Micronization and Bio-activated (FMB) extract of Gymnema sylvestre for higher antidiabetic and antioxidant effects compared to its conventional standardized extract

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