Comparative interleukins and chemokines analysis of mice mesenchymal stromal cells infected with Mycobacterium tuberculosis H37Rv and H37Ra

Li, Heng; Cao, Wei; Chen, Shichao; Chen, Jianxia; Xing, Yan; Yang, Hong

doi:10.1016/j.abb.2023.109673

Archives of Biochemistry and Biophysics

2023

DOI: 10.1016/j.abb.2023.109673

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Comparative interleukins and chemokines analysis of mice mesenchymal stromal cells infected with Mycobacterium tuberculosis H37Rv and H37Ra

Heng Li

Wei Cao

Shichao Chen

et al.

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2024

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The proteomic changes induced by Norcantharidin were quantified using LC-MS/MS in macrophages infected with Mycobacterium tuberculosis

Wu,

Li,

Liu

et al. 2024

Preprint

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Tuberculosis drug resistance contributes to the spread of tuberculosis. Immunotherapy is an effective strategy for treating tuberculosis, with the regulation of macrophage-mediated anti-tuberculosis immunity being crucial. Norcantharidin (NCTD), a drug used in tumor immunotherapy, has significant immunomodulatory effects. Thus, NCTD may have an anti-tuberculosis role by regulating immunity. Understanding how NCTD affects the proteome of Mtb-infected macrophages can provide valuable insights into potential treatments. This study aimed to investigate the impact of NCTD (10 μg/mL) on the proteome of macrophages infected with Mtb H37Ra using liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis. A total of 69 differentially regulated proteins (DRPs) were identified, with 28 up-regulated and 41 down-regulated in the NCTD-treated group. Validation of six DRPs (CLTCL1, VAV1, SP1, TRIM24, MYO1G, and WDR70) by Western blot analysis confirmed the accuracy of the LC-MS/MS method used in this study. NCTD modulates various protein expressions involved in chromatin-modifying enzymes, RHO GTPases activating PAKs, Fc gamma R-mediated phagocytosis, T cell receptor signaling pathway, and antigen processing and presentation. Overall, the research provides new insights into the effects of NCTD on the proteome of Mtb-infected macrophages. The identified changes highlight potential targets for future therapeutic interventions aimed at enhancing host immunity against Mtb infection or developing new anti-TB drugs based on these findings.

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The proteomic changes induced by Norcantharidin were quantified using LC-MS/MS in macrophages infected with Mycobacterium tuberculosis

Wu,

Li,

Liu

et al. 2024

Preprint

View full text Add to dashboard Cite

show abstract

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Comparative interleukins and chemokines analysis of mice mesenchymal stromal cells infected with Mycobacterium tuberculosis H37Rv and H37Ra

Cited by 1 publication

References 38 publications

The proteomic changes induced by Norcantharidin were quantified using LC-MS/MS in macrophages infected with Mycobacterium tuberculosis

The proteomic changes induced by Norcantharidin were quantified using LC-MS/MS in macrophages infected with Mycobacterium tuberculosis

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