Comparative Infectivity for Axenic and Specific-Pathogen-Free Chickens of O2 Escherichia coli Strains with or without Virulence Factors

Brée, Annie; Dho, Maryvonne; Lafont, Jean-Pierre

doi:10.2307/1591078

Cited by 32 publications

(6 citation statements)

References 16 publications

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“…The frequency of these two virulence markers among the isolates of E. coli appears low, with 1.6% producing mucoid colonies and 3.6% hemolytic. However, the possibility of expression of other virulence markers, not assayed for, cannot be ignored (32)(33)(34)(35).…”

Section: Discussionmentioning

confidence: 99%

Microbial quality of water in rural communities of Trinidad

Welch¹,

David²,

Clarke³

et al. 2000

Rev Panam Salud Publica

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A cross-sectional study was conducted in four rural communities of northeastern Trinidad to determine the microbial quality of water supply to households and that quality's relationship to source and storage device. Of the 167 household water samples tested, total coliforms were detected in 132 of the samples (79.0%), fecal coliforms in 102 (61.1%), and E. coli in 111 (66.5%). There were significant differences among the towns in the proportion of the samples contaminated with coliforms (P < 0.001) and E. coli (P < 0.001). Of 253 strains of E. coli studied, 4 (1.6%) were mucoid, 9 (3.6%) were hemolytic, and 37 (14.6%) were nonsorbitol fermenters. Of 69 isolates of E. coli tested, 10 (14.5%) were verocytotoxigenic. Twenty-eight (14.0%) of 200 E. coli isolates tested belonged to enteropathogenic serogroups. Standpipe, the most common water source, was utilized by 57 (34.1%) of the 167 households. Treated water (pipeborne in homes, standpipes, or truckborne) was supplied to 119 households (71.3%), while 48 households (28.7%) used water from untreated sources (rain, river/stream, or well) as their primary water supply. The type of household storage device was associated with coliform contamination. Water stored in drums, barrels, or buckets was more likely to harbor fecal coliforms (74.2% of samples) than was water stored in tanks (53.3% of samples), even after controlling for water source (P = 0.04). Compared with water from other sources, water piped into homes was significantly less likely to be contaminated with total coliforms (56.9% versus 88.8%, P < 0.001) and fecal coliforms (41.2% versus 69.8%, P < 0.01), even when the type of storage device was taken into account. However, fecal contamination was not associated with whether the water came from a treated or untreated source. We concluded that the drinking water in rural communities in Trinidad was grossly unfit for human consumption, due both to contamination of various water sources and during household water storage.

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Section: Discussionmentioning

confidence: 99%

Microbial quality of water in rural communities of Trinidad

Welch¹,

David²,

Clarke³

et al. 2000

Rev Panam Salud Publica

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“…Axenic strain PA12 White Leghorn chicks were obtained from the INRA infectiology platform by using the method described by Le Bars (30). Specific-pathogen-free (SPF) chickens were obtained by orally inoculating 8-day-old axenic chicks with a complex bacterial inoculum consisting of 0.5 ml of a 1/10 suspension of feces from adult SPF hens (6). The E. coli strains in this inoculum were all antibiotic sensitive.…”

Section: Methodsmentioning

confidence: 99%

A Genomic Island of an Extraintestinal Pathogenic Escherichia coli Strain Enables the Metabolism of Fructooligosaccharides, Which Improves Intestinal Colonization

et al. 2009

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Prebiotics such as fructooligosaccharides (FOS) are increasingly being used in some countries for improving human and animal health and as an alternative to antibiotic growth promoters in animals, with various degrees of success. It has been observed that FOS stimulate the proliferation of probiotic bacteria and, at the same time, decrease the population of bacteria associated with disease. This observation assumes that pathogenic bacteria do not metabolize FOS and, therefore, lose their competitive advantage over beneficial bacteria. Here we present evidence that some pathogenic Escherichia coli strains can metabolize FOS and show that this property helps the bacterium colonize the intestine. These findings highlight the potential risk that a high level of prebiotic usage could lead to the emergence of well-adapted pathogenic strains that metabolize prebiotic substances.

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“…Although 7 of the 22 SHS isolates studied here had 02 or 078 antigens, two of the common 0 serogroups associated with avian colibacillosis (7,35), the role of these cell surface lipopolysaccharides in disease is undetermined. In conjunction with clonal analysis, isolates need to be examined for putative virulence factors, such as capsule or hemolysin production (5,14), and tested in experimental infections to define the bacterial factors involved in SHS pathogenesis (23). Virtually nothing is known about the virulence factors in SHS-associated E. coli strains, but the following comparison with human pathogenic strains suggests some directions for future research on SHS.…”

Section: Etmentioning

confidence: 99%

Genetic relationships among strains of avian Escherichia coli associated with swollen-head syndrome

et al. 1990

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Genetic diversity among 22 Escherichia coli strains isolated from chickens with swollen-head syndrome (SHS), an acute respiratory disease of domestic poultry, and 93 strains isolated from birds with colibacillosis was assessed on the basis of allelic variation at 20 enzyme-encoding loci detected by multilocus enzyme electrophoresis. SHS isolates from Spain and Canada were polymorphic at 14 loci and were classified into 19 multilocus genotypes, defining clones that differed on average at 34% of the loci. In most cases, SHS isolates of different clonal genotypes were distinct in O:H serotype and expressed different fimbrial antigens. Comparisons with 93 isolates obtained from birds with colibacillosis revealed enzyme polymorphisms at 17 of 20 loci, with an average of 3.5 alleles per locus. In the total sample, 56 clonal genotypes were distinguished, with 27 (23%) of the isolates belonging to one of three common clones. Both SHS and colibacillosis isolates were genetically diverse, with an average single-locus diversity of 0.36, indicating that a wide variety of naturally occurring bacterial clones is associated with these acute avian infections. Six previously defined groups of clones identified in diseased birds from the United States were represented in isolates from Spain, indicating that similar clones occur in widely separated geographic areas. In addition, one group of SHS isolates was closely related to a recognized widespread clone complex incriminated in human septicemia and meningitis. The results suggest that certain strains implicated in SHS infections belong to a clone complex whose members have special attributes that promote involvement in invasive diseases in humans and animals.

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Comparative Infectivity for Axenic and Specific-Pathogen-Free Chickens of O2 Escherichia coli Strains with or without Virulence Factors

Cited by 32 publications

References 16 publications

Microbial quality of water in rural communities of Trinidad

Microbial quality of water in rural communities of Trinidad

A Genomic Island of an Extraintestinal Pathogenic Escherichia coli Strain Enables the Metabolism of Fructooligosaccharides, Which Improves Intestinal Colonization

Genetic relationships among strains of avian Escherichia coli associated with swollen-head syndrome

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