COMPARATIVE in vitro EVALUATION OF APHERESIS PLATELETS STORED WITH 100% PLASMA VERSUS BICARBONATED RINGER'S SOLUTION WITH LESS THAN 5% PLASMA

Administration of platelet concentrates suspended in bicarbonated Ringer's solution in children who had platelet transfusion reactions

“…Washed PCs were prepared at the Nagano Children's Hospital according to the guidelines of the Japan Society of Transfusion Medicine and Cell Therapy . BRS‐A and G‐sol as PASs were prepared based on a method described previously . Chlorpheniramine maleate was administered 5 min before PC transfusion in all cases, including the control group.…”

In vitro variables of buffy coat–derived platelet concentrates with residual plasma of down to 10% are stably maintained in new‐generation platelet additive solutions

“…Glucose was added as metabolic substrate because most glucose is removed from genuine plasma during processing. These new PASs, marketed as PAS‐G (Pall Corp., Covina, CA) and M‐Sol, BRS‐A, PAS‐5, and Intersol‐G (Fresenius Kabi, Bad Homburg, Germany), may support additional reduction of residual plasma to 20% or below while maintaining in vitro PLT quality at levels equivalent or superior to those of PLTs stored in plasma with non–glucose‐containing PASs, such as SSP+ (Macopharma, Tourcoing, France) . Glucose is important for maintaining ATP levels and NADPH‐reducing equivalents and was shown to be essential for the maintenance of PLTs stored as concentrates with minimal volumes of plasma .…”

Impact of the platelet washing process on in vitro platelet properties, and the levels of soluble CD40 ligand and platelet‐derived microparticles in the storage media

“…The HSR was determined at 5 minutes using a standard spectrophotometer (Multiskan GO, Thermo Fisher Scientific, Inc.) . Surface glycoprotein expression levels of CD62P (P‐selectin) and CD42b (glycoprotein Ib alpha) were detected by flow cytometry as previously described . For detecting spontaneous expression of the conformational epitope on the GPIIb/IIIa complex of PLTs, a mouse monoclonal anti‐human PAC‐1 antibody was used, as previously described .…”

“…Immediately prior to PLT washing, BRS‐A was prepared as previously described . For PLT washing, 250 mL of BRS‐A and 25 mL of ACD‐A were added to PCs, and the mixture was centrifuged at 1,500 g for 20 minutes at 22°C.…”

“…Despite these advantages, the preparation of M‐sol includes laborious procedures, which requires special education and training. Therefore, we have developed a novel PAS, bicarbonated Ringer's solution (BRS) supplemented with acid‐citrate‐dextrose Formula A (ACD‐A), termed BRS‐A, which can preserve in vitro PLT properties for at least 7 days with less than 5% plasma . Unlike M‐sol, BRS‐A can be prepared by simply mixing the two infusions.…”