Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum

Oliveira, M. B.; Barbosa, Simone C.; Petrofeza, Silvana

doi:10.4238/2013.june.6.3

Cited by 8 publications

(4 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Plant cell walls are the first barrier against pathogenic fungal invasion [60]. To overcome the cell wall, S. sclerotiorum secretes numerous cell-wall-degrading enzymes (CWDEs) such as polygalacturonases, exo-β-1,3-glucanases, xylanases, and cellulases, which are detected during the early stages of infection [61]. In addition, cutinases are characterized as extracellular serine esterases that break the ester bond of cutin from the cuticle of plant [44].…”

Section: Discussionmentioning

confidence: 99%

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Zhou

Huang

et al. 2021

JoF

View full text Add to dashboard Cite

Microbial secondary metabolites produced by Streptomyces are applied to control plant diseases. The metabolite, ε-poly-L-lysine (ε-PL), is a non-toxic food preservative, but the potential application of this compound as a microbial fungicide in agriculture is rarely reported. In this study, the effect and mode of action of ε-PL on two necrotrophic pathogenic fungi, Sclerotinia sclerotiorum and Botrytis cinerea, were investigated. The results showed that ε-PL effectively inhibited the mycelial growth of S. sclerotiorum and B. cinerea with EC50 values of 283 μg/mL and 281 μg/mL, respectively. In addition, ε-PL at the dose of 150 and 300 μg/mL reduced S. sclerotiorum sclerotia formation. The results of the RNA-seq and RT-qPCR validation indicated that ε-PL significantly regulated the gene expression of critical differential expressed genes (DEGs) involved in fungal growth, metabolism, pathogenicity, and induced an increase in the expression of the fungal stress responses and the detoxification genes. These results provided new insights for understanding the modes of action of ε-PL on S. sclerotiorum and B. cinerea and improved the sustainable management of these plant diseases.

show abstract

Section: Discussionmentioning

confidence: 99%

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Zhou

Huang

et al. 2021

JoF

View full text Add to dashboard Cite

show abstract

“…Degradation of plant cell wall is an important aspect of pathogenesis. Pathogenic fungi secrete numerous enzymes for degrading cell wall such as polygalacturonases, exo-β-1,3-glucanases, xylanases and cellulases during the early stages of infection4142. For example, chitinase is important for F. oxysporum virulence by hydrolyzing β-1,4 linkages in chitin polymers43.…”

Section: Discussionmentioning

confidence: 99%

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Zhang

Wang

Shu

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Soil degradation is a serious global problem, but little is known about how soil microbial communities respond to soil degradation as well as their feedback to ecosystem functioning. In this study, we found the microbial community composition, structure and functional potential significantly altered in the degraded soils with bacterial wilt (termed as degraded soils). Compared with healthy soils, OTU richness of beneficial microorganisms were significantly decreased, but OTU richness of pathogenic microorganisms were significantly increased in the degraded soils. Functional gene array (GeoChip 5.0) analysis showed the functional metabolic potential of genes involved in stress, virulence, sulfur cycle, metal resistance, degradation of plant cell wall was significantly increased in the degraded soils. Increased functional metabolic potential of these genes may be related to the acidification and severe plant disease of degraded soils. Biological activity of degraded soils was obviously decreased with weakened soil enzyme activities when compared to the healthy soils. Soil pH and enzyme activities were negatively correlated with the abundance of genes involved in sulfur cycle, virulence, and stress responses. This study provides new insights into our understanding of soil microbial community responses to soil degradation.

show abstract

“…The infection process and establishment of compatible interactions have also been well characterized at the cytological level ( Lumsden, 1979 ; Lumsden and Wergin, 1980 ; Tariq and Jeffries, 1986 ). Differential accumulation of specific defense-related transcripts, such as mRNA for polygalacturonase-inhibiting protein (PGIP) and pathogen related proteins, during the S. sclerotiorum – P. vulgaris interaction has also been reported ( Oliveira et al, 2010 , 2013 ; Kalunke et al, 2011 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction

et al. 2015

View full text Add to dashboard Cite

The fungus Sclerotinia sclerotiorum (Lib.) de Bary, one of the most important plant pathogens, causes white mold on a wide range of crops. Crop yield can be dramatically decreased due to this disease, depending on the plant cultivar and environmental conditions. In this study, a suppression subtractive hybridization cDNA library approach was used for the identification of pathogen and plant genes that were differentially expressed during infection of the susceptible cultivar BRS Pérola of Phaseolus vulgaris L. A total of 979 unigenes (430 contigs and 549 singletons) were obtained and classified according to their functional categories. The transcriptional profile of 11 fungal genes related to pathogenicity and virulence were evaluated by reverse transcription quantitative real-time polymerase chain reaction (RT-qPCR). Additionally, the temporal expression profile obtained by RT-qPCR was evaluated for the following categories of plant defense-related genes: pathogenesis-related genes (PvPR1, PvPR2, and PvPR3), phenylpropanoid pathway genes (PvIsof, PvFPS1, and 4CL), and genes involved in defense and stress-related categories (PvLox, PvHiprp, PvGST, PvPod, and PvDox). Data obtained in this study provide a starting point for achieving a better understanding of the pathosystem S. sclerotiorum–P. vulgaris.

show abstract

Comparative in vitro and in planta analyses of extracellular enzymes secreted by the pathogenic fungus Sclerotinia sclerotiorum

Cited by 8 publications

References 23 publications

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

ε-poly-L-lysine Affects the Vegetative Growth, Pathogenicity and Expression Regulation of Necrotrophic Pathogen Sclerotinia sclerotiorum and Botrytis cinerea

Microbial taxa and functional genes shift in degraded soil with bacterial wilt

Analysis of genes that are differentially expressed during the Sclerotinia sclerotiorum–Phaseolus vulgaris interaction

Contact Info

Product

Resources

About