Comparative in silico and protein-protein interaction network analysis of ATP1A1 gene

Sahoo, Siddhant Sekhar; Mishra, Chinmoy; Rout, Mangalika; Nayak, Gangadhar; Mohanty, Stuti T; Panigrahy, Kuldeep Kumar

doi:10.1016/j.genrep.2016.10.004

Cited by 2 publications

(2 citation statements)

References 28 publications

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“…ATP1A1 is extensively studied in exercise physiology and inactivity in humans [ 73 ]. ATP1A1 interacts with other proteins and modulates hypertension and feed intake [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle

Elayadeth-Meethal

Veettil

Asaf

et al. 2021

Animals

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Climate change is an imminent threat to livestock production. One adaptation strategy is selection for heat tolerance. While it is established that the ATP1A1 gene and its product play an important role in the response to many stressors, there has been no attempt to characterize the sequence or to perform expression profiling of the gene in production animals. We undertook a field experiment to compare the expression profiles of ATP1A1 in heat-tolerant Vechur and Kasaragod cattle (Bos taurus indicus) with the profile of a heat-susceptible crossbreed (B. t. taurus × B. t. indicus). The cattle were exposed to heat stress while on pasture in the hot summer season. The environmental stress was quantified using the temperature humidity index (THI), while the heat tolerance of each breed was assessed using a heat tolerance coefficient (HTC). The ATP1A1 mRNA of Vechur cattle was amplified from cDNA and sequenced. The HTC varied significantly between the breeds and with time-of-day (p < 0.01). The breed–time-of-day interaction was also significant (p < 0.01). The relative expression of ATP1A1 differed between heat-tolerant and heat-susceptible breeds (p = 0.02). The expression of ATP1A1 at 08:00, 10:00 and 12:00, and the breed–time-of-day interaction, were not significant. The nucleotide sequence of Vechur ATP1A1 showed 99% homology with the B. t. taurus sequence. The protein sequence showed 98% homology with B. t. taurus cattle and with B. grunniens (yak) and 97.7% homology with Ovis aries (sheep). A molecular clock analysis revealed evidence of divergent adaptive evolution of the ATP1A1 gene favoring climate resilience in Vechur cattle. These findings further our knowledge of the relationship between the ATP1A1 gene and heat tolerance in phenotypically incongruent animals. We propose that ATP1A1 could be used in marker assisted selection (MAS) for heat tolerance.

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“…ATP1A1 is extensively studied in exercise physiology and inactivity in humans [ 73 ]. ATP1A1 interacts with other proteins and modulates hypertension and feed intake [ 74 ].…”

Section: Discussionmentioning

confidence: 99%

Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle

Elayadeth-Meethal

Veettil

Asaf

et al. 2021

Animals

View full text Add to dashboard Cite

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“…The theoretical pH was found 5.61 to 5.76 in all the animals which indicate this protein is acidic in nature. It may be due to large number glutamic acid and aspartic acid in this protein Sahoo et al (2016). The instability index was found above 40 of all the proteins which indicates this protein is unstable in nature in all selected animals Guruprasad et al (1990).The aliphatic index was found above 70 indicating this protein is thermo stable which may be due to presence of more aliphatic side chain amino acids (Ikai, 1980).…”

Section: Physiochemical Analysismentioning

confidence: 99%

Comparative Insilco physiochemical and phylogenetic analysis of Insulin like growth factor 1 receptor (IGF-1R) in domestic animals

Sahoo

Behera

2018

IJAR

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Insulin like growth factor 1receptors (IGF-1R) are the proteins which are expressed on the cell surface of almost all tissues in human as well as domestic animals with major involvement in growth, cancer, aging, production and in early embryonic development. Due to above importance, this protein needs to be characterized both in physiochemical and phylogenetically for further exploration in livestock research. In this study, the IGF1R amino acid sequences of selected domestic animals are retrieved from UniProt database and various physiochemical parameters were compared through ProtParam insilco tool. The multiple sequence alignment (MSA) and phylogenetic analysis was performed through Clustal omega and Molecular evolutionary genetics analysis (MEGA) application platform respectively. It was found that this protein is an unstable, hydrophilic in all domestic animals with amino acids varied from 1307 to 1412 in number. The phylogenetic analysis showed that highest time of divergence occurs in killer whale and rabbit, but least time of divergence occurs between goat and bovine. So this study will provide a better platform for the development of suitable anticancer therapeutics in domestic animals in nearest future as IGF-1R is implicated in several cancers, including breast, prostate, and lung cancers.

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Comparative in silico and protein-protein interaction network analysis of ATP1A1 gene

Cited by 2 publications

References 28 publications

Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle

Comparative Expression Profiling and Sequence Characterization of ATP1A1 Gene Associated with Heat Tolerance in Tropically Adapted Cattle

Comparative Insilco physiochemical and phylogenetic analysis of Insulin like growth factor 1 receptor (IGF-1R) in domestic animals

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