Comparative immunological and chromatographic study of some plant β‐Amylases

Abstract

A comparative study has been made of the β‐amylases of barley, wheat, rye, oats and sweet‐potato by means of exclusion chromatography and immunochemical analysis. The reactivity of barley malt and wheat β‐amylase was compared with different anti‐barley and anti‐wheat sera. In exclusion chromatography on Sephadex G100, barley β‐amylase yielded four, and both wheat and rye, two active components, whereas oat and sweet‐potato had only one active component. During the storage of barley, wheat and rye β‐amylases the large‐molecule components were split into smaller ones; no changes occurred in oat and sweet‐potato β‐amylases. On analysis against a specific barley β‐amylase antiserum, wheat and rye β‐amylase gave a reaction which indicated that they were immunologically partly identical with barley β‐amylase, and identical with each other. This serum induced no reaction in β‐amylases of sweet‐potato and oats. The rye β‐amylase precipitation line did not display enzymic activity after reaction with this antiserum. Analyses with different antisera of barley and wheat confirmed the partial immunological identity of barley malt and wheat β‐amylase. With some barley antisera, partial inhibition of wheat β‐amylase activity was observed. A similar phenomenon was apparent when barley malt β‐amylase was precipitated with some wheat antisera.

Immunochemical characterization of protein in plant studies

“…In earlier studies, Nummi (1967) demonstrated the existence of a polymorphism related to molecular size in the free barley /3-amylase. This heterogeneity in molecular size is found in several cereals (Nummi et al, 1970) and includes, for barley, some constituents which could be transformed into each other by reduction and partly by oxidation (Nummi, 1967). A further structural identification between these constituents and with the bound /3-amylase was provided by I.E.A.…”

发育过程中苹果果实的<I>β</I>-淀粉酶：活性、数量变化和亚细胞定位

“…Ҳ̾ ȴ Ҳβ-ȴ 50~60 ku Ҳ . [4,19,34,48,49] , + ɹƵ‫܈‬ ܵ ȴ \ҲщN [50] . Хt« Ҳβ-Қ 4 ȴ 50 ku ҲƖ ֖ Ҳ̈‫.ץ‬ [51,52] , 3 P Ɩ Ҳ cDNA 8ԂҲۤ ‫ݳ‬Ҳ ։8, ̾ D Ҳβ- [23] .…”