Comparative Immunohistochemical Study of the Distribution of Fibronectin in Healthy and Diseased Root Surfaces

Komboli, Mado G.; Kodovazenitis, George; Katsorhis, Theodore

doi:10.1902/jop.2009.080549

Cited by 5 publications

(5 citation statements)

References 44 publications

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“…Because of these important roles, FN has been evaluated for use in periodontal regenerative therapies [26, 27] and bone repair processes [28, 29]. FN has been reported in bone, predentin, and dentin [22], and differentially distributed in the cementum matrix [30]. In the present investigation, FN was found at a 33-fold greater concentration in AB versus DC, perhaps suggesting a different or more critical role for FN in AB.…”

Section: Resultsmentioning

confidence: 99%

Proteomic analysis of human dental cementum and alveolar bone

Salmon

Tomazela

Silvério

et al. 2013

Journal of Proteomics

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Dental cementum (DC) is a bone-like tissue covering the tooth root and responsible for attaching the tooth to the alveolar bone (AB) via the periodontal ligament (PDL). Studies have unsuccessfully tried to identify factors specific to DC versus AB, in an effort to better understand DC development and regeneration. The present study aimed to use matched human DC and AB samples (n=7) to generate their proteomes for comparative analysis. Bone samples were harvested from tooth extraction sites, whereas DC samples were obtained from the apical root portion of extracted third molars. Samples were denatured, followed by protein extraction reduction, alkylation and digestion for analysis by nanoAcquity HPLC system and LTQ-FT Ultra. Data analysis demonstrated that a total of 318 proteins were identified in AB and DC. In addition to shared proteins between these tissues, 105 and 83 proteins exclusive to AB or DC were identified, respectively. This is the first report analyzing the proteomic composition of human DC matrix and identifying putative unique and enriched proteins in comparison to alveolar bone. These findings may provide novel insights into developmental differences between DC and AB, and identify candidate biomarkers that may lead to more efficient and predictable therapies for periodontal regeneration.

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Section: Resultsmentioning

confidence: 99%

Proteomic analysis of human dental cementum and alveolar bone

Salmon

Tomazela

Silvério

et al. 2013

Journal of Proteomics

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“…For example, bone sialoprotein (BSP) and osteopontin, both rich in healthy cementum matrix, were found to be absent in cementum exposed to periodontal pockets . Fibronectin showed variation in its distribution and fibrillar structure in pocket cementum compared to healthy cementum . Undoubtedly, these pathological changes of diseased cementum may influence cementum regeneration and connective tissue attachment to root surfaces.…”

Section: Introductionmentioning

confidence: 99%

“…However, during the process of periodontitis, root cementum becomes exposed to periodontal pockets. In addition to contamination by bacterial plaque and endotoxin , cementum suffers loss of active substances due to periodontal inflammation . For example, bone sialoprotein (BSP) and osteopontin, both rich in healthy cementum matrix, were found to be absent in cementum exposed to periodontal pockets .…”

Section: Introductionmentioning

confidence: 99%

Effects of conservatively treated diseased cementum with or without EMD on in vitro cementoblast differentiation and in vivo cementum‐like tissue formation of human periodontal ligament cells

Qi¹,

Feng

Cai

et al. 2014

Cell Proliferation

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“…Structural and compositional changes in the matrix components of cementum have been reported in patients with periodontitis. [12] Significant evidence supports a strong correlation between periodontal disease and diseased or altered cementum.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of thickness of cementum of periodontally diseased teeth of non-diabetic and type 2 diabetic patients: A scanning electron microscopy study

Gupta¹,

Galgali²,

Bavle³

et al. 2013

J Indian Soc Periodontol

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Background:It is suggested that diabetes plays an important role in tooth loss. The periodontal structure and alveolar bone architecture in diabetics have revealed alterations. However, changes in cementum of diabetics with periodontal disease have not been evaluated adequately. Thus, the aim of the study is to evaluate and compare the thickness of cementum in diseased and healthy areas of roots of teeth with chronic periodontitis from non-diabetic (group I) and type 2 diabetic patients (group II).Materials and Methods:Thirty incisors indicated for extraction as a result of chronic periodontitis from group I and group II were selected. Measurements of Probing Depth (PD) and Clinical Attachment Loss (CAL) were taken prior to extractions. Area of the root surface above the CAL (notch) was designated as diseased, while the area below the CAL (notch) was designated as healthy. The extracted tooth samples were sectioned longitudinally and were evaluated by scanning electron microscopy (SEM). Descriptive statistical analysis was performed. Results of continuous measurements were presented as Mean ± SD.Results:The results showed that in both groups the thickness of cementum in diseased areas was lower in comparison to the thickness in healthy areas. The mean thickness of cementum in both healthy and diseased areas was higher in group II compared to group I, though the differences in both were not statistically significant.Conclusion:Within the limits of the present study, group II showed greater mean thickness of cementum in both healthy and diseased areas compared to group I, though the differences were not statistically significant.

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Comparative Immunohistochemical Study of the Distribution of Fibronectin in Healthy and Diseased Root Surfaces

Abstract: Changes in cementum due to periodontitis include changes in the distribution and morphology of fibronectin. These changes may influence the ability for regeneration and connective tissue attachment onto periodontally affected root surfaces.

Cited by 5 publications

References 44 publications

Proteomic analysis of human dental cementum and alveolar bone

Proteomic analysis of human dental cementum and alveolar bone

Effects of conservatively treated diseased cementum with or without EMD on in vitro cementoblast differentiation and in vivo cementum‐like tissue formation of human periodontal ligament cells

Evaluation of thickness of cementum of periodontally diseased teeth of non-diabetic and type 2 diabetic patients: A scanning electron microscopy study

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