Comparative immunohistochemical investigation of markers for malignant histiocytes

Roholl, P. J. M.; Kleyne, J.; Pijpers, Helga W.; Unnik, J. A. M. van

doi:10.1016/s0046-8177(85)80247-1

Cited by 45 publications

(16 citation statements)

References 29 publications

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“…In the present study, several commercially available histiocytic markers were applied to all cases. Lysozyme and α1-antitrypsin are classical histiocytic markers [17,19]. Lysozyme is reported as a useful marker to identify macrophages, however, while the absence of lysozyme antigen does not necessarily preclude a histiocytic origin [17].…”

Section: Discussionmentioning

confidence: 99%

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

Suzuki

Uchida

Morozumi

et al. 2003

The Journal of Veterinary Medical Science

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ABSTRACT. Histiocytic proliferative disorders in canine central nervous system (CNS) including granulomatous meningoencephalomyelitis (GME) and malignant histiocytosis were compared pathologically. Lesions of GME mainly existed in the white matter of the cerebr um, brainstem and cerebellum and consisted of characteristic perivascular cuffing, parenchymal granuloma and leptomeningeal infiltrates of mononuclear cells. In malignant histiocytosis, there were two histological patterns, diffuse proliferation of neoplastic histiocytes through the leptomeninges and neoplastic nodule formation in the parenchyma. Neoplastic histiocytes exhibited mild to severe cellular atypia and high ability of invasion into the brain parenchyma. Mitotic and phagocytic figures were also observed. Several histiocytic markers, including lysozyme, α1-antitrypsin and lectin RCA-1, revealed histiocytic origin of both inflammatory and neoplastic cells, however, those were not determinative for the discrimination between GME and malignant histiocytosis. CD3-and PCNA-positive cells existed in the lesions of both diseases. The number of CD3-positive cells in GME tended to be greater than in malignant histiocytosis, while the difference was not statistically significant.

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Section: Discussionmentioning

confidence: 99%

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

Suzuki

Uchida

Morozumi

et al. 2003

The Journal of Veterinary Medical Science

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“…20 The utility oflectins (SBA, PNA, and ConA) as probes for identifying mononuclear phagocytes and their malignant derivatives has been investigated previously. 4,13,32 In addition to these lectins, binding with WGA was demonstrated in the Golden Retriever. Detection of lectin receptors in malignant analogs of histiocytes appears to be independent of the degree of differentiation of tumor cells; this independence may assist in the identification of tumors with an intermediate degree of differentiation where markers such as lysozyme may be absent.…”

Section: Discussionmentioning

confidence: 99%

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

et al. 1993

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Diagnosis of malignant histiocytosis (MH), a disorder characterized by systemic proliferation of morphologically atypical histiocytes and their precursors, in an 8-year-old neutered female Golden Retriever was based on light and electron microscopic and immunohistochemical findings. Clinically, the dog presented with unilateral forelimb lameness. Eight days after surgical exploration of a swollen brachium, the dog developed sudden onset of posterior paresis, fecal and urinary incontinence, and a flaccid tail. Necropsy revealed infiltrative and nodular lesions in the right forelimb and regional lymph nodes, thoracic and abdominal cavities, and lumbar epidural space. Gross lesions were not found in the lungs or integument. Histopathologic examination showed infiltrates of atypical histiocytes in skeletal muscle, joint, and regional lymph nodes of the right forelimb; intercostal muscle; lung; liver; spleen; pancreas; kidneys; and spinal dura. Most tumor infiltrates were nodular and composed of loosely aggregated cells that were 10-30 μm in diameter with abundant eosinophilic to foamy cytoplasm, had central or eccentric nuclei, and were periodic acid-Schiff negative. Many binucleated cells, multinucleated giant cells, and mitotic figures were seen. Tumor cells contained phagocytosed erythrocytes, mononuclear cells, and some leukocytes. Ultrastructural features of tumor cells included cytoplasmic lipid droplets, lysosomes, and phagolysosomes. Immunohistochemical studies on paraffin-embedded sections showed positive reactivity to human T-cell Ag (clone UCHL-1) and for lysozyme, α-1-antitrypsin, and cathespin B. Polyclonal intracellular immunoglobulin reactivity and lectin binding (peanut, soybean, and wheat germ agglutinins and concanavalin A) were also demonstrated. Criteria for diagnosis of malignant histiocytic tumors and differential diagnosis are discussed.

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“…EM studies were performed on the original nephrectomy specimen of case 1 and the transplant nephrectomy specimens of both cases. Par affin sections (one each) from the original kidney (case 1) and the transplanted kidneys (both cases) were stained by the avidin-biotinperoxidase technique for muramidase and ai-antichymotrypsin us ing primary antibody from Dako and Victor ABC reagent kits [16][17][18][19]. The number of intraglomerular mononuclear cells positive for muramidase was ascertained for a single slide from each specimen and divided by the total number of glomeruli in the slide to obtain a mononuclear muramidase index similar to the nonspecific esterase index of Magi!…”

Section: Methods and Case Reportsmentioning

confidence: 99%

Recurrent Focal Segmental Glomerulosclerosis

Verani

Hawkins²

1986

Am J Nephrol

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We describe the early lesion of focal segmental glomerulosclerosis (FSGS) as it appeared in 2 cases of recurrent FSGS with early transplant nephrectomies (approximately 2 months) due to uncontrollable nephrotic syndrome. A quantitative evaluation showed that these lesions were present more often in the glomeruli of the juxtame-dullary cortex. Epithelial cell proliferation and detachment and foam cells were seen frequently and were considered the hallmarks of the early lesion of FSGS. Hyaline deposits are secondary late phenomena. We suggest that the glomerular foam cells in FSGS are mesangial in origin.

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Comparative immunohistochemical investigation of markers for malignant histiocytes

Cited by 45 publications

References 29 publications

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

A Comparative Pathological Study on Granulomatous Meningoencephalomyelitis and Central Malignant Histiocytosis in Dogs

Disseminated Malignant Histiocytosis in a Golden Retriever: Clinicopathologic, Ultrastructural, and Immunohistochemical Findings

Recurrent Focal Segmental Glomerulosclerosis

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