Comparative immunohistochemical analysis between jaw myxoma and mesenchymal cells of tooth germ

Lombardi, Tommaso; Samson, Jacky; Bernard, Jean‐Pierre; Felice, Roberto Di; Fiore-Donno, G; Wülhauser, J.; Maggiano, Nicola

doi:10.1016/s0344-0338(11)81170-2

Cited by 18 publications

(12 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These results were in agreement with a previous study [24] which failed to demonstrate S-100 protein cells in embryonic rat dental papillae. In a recent work we have also demonstrated the absence of S-100 protein in human dental papilla [25], The presence of S-100 protein in the odontoblasts of human tooth germs has also been reported [26,27] as well as in teeth of some rodents [28]. In the present study with the technical condi tions used, the odontoblasts were immunonegative for S-100 protein.…”

Section: Discussionsupporting

confidence: 78%

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

Lombardi¹,

Fiore-Donno²

1993

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

Human teeth (formalin-fixed, demineralized and paraffin-embedded as well as frozen sections of human dental pulp) were examined by immunohistochemistry to detect the presence and distribution of a series of neural and neural-crest-associated antigens: S-100 protein, neuron-specific enolase (NSE), glial fibrillary acid protein (GFAP), myelin basic protein (MBP) and neurofilaments (NF). Although several experimental pieces of evidence support a neural crest derivation for dental papilla and odontoblasts with the histological method used in the present study, these structures were immunonegative for most of the markers employed in the present investigation. Indeed, mesenchymal cells as fibroblasts and endothelial cells were devoid of immunostaining. Schwann cells were strongly immunostained by S-100, NSE and GFAP. Nerve fibres running in the pulp showed a positive labelling for NF, S-100 and MBP antisera. It is concluded that the lack of labelling of pulp cells (excepting nerves) by the neural and neural-crest-associated antibodies could be due to a loss of capacity to express these antigens during differentiation. However, the possibility that these antigenic determinants are expressed but outside the limits of sensitivity of immunohistochemistry cannot be ruled out. Further studies, including molecular biology techniques, are required to clarify these alternatives.

show abstract

Section: Discussionsupporting

confidence: 78%

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

Lombardi¹,

Fiore-Donno²

1993

Cells Tissues Organs

Self Cite

View full text Add to dashboard Cite

show abstract

“…These tumors have histologic features of other myxomas (Lombardi et al, 1992;Quintal et al, 1994), such as those of cardiac and skin tissue (Acebo et al, 2003;Amano et al, 2003). OMs are rare tumors that do not occur as part of CNC .…”

Section: Discussionmentioning

confidence: 99%

“…OMs are locally invasive and have a propensity to recur frequently, especially if incompletely resected (Kaffe et al, 1997;Hisatomi et al, 2003). Microscopically, this tumor is composed of loosely arranged stellate, spindle-shaped, round cells in an abundant, loose myxoid stroma that contains only a few collagen fibrils; small islands of inactive-appearing odontogenic epithelial rests may be scattered throughout the myxoid ground substance (Lombardi et al, 1992;Quintal et al, 1994).…”

Section: Introductionmentioning

confidence: 99%

Molecular and immunohistochemical investigation of protein kinase a regulatory subunit type 1A (PRKAR1A) in odontogenic myxomas

Perdigão

Stergiopoulos

Marco

et al. 2005

Genes Chromosomes & Cancer

View full text Add to dashboard Cite

Odontogenic myxomas are rare benign neoplasms affecting the jaw. Myxomas of bones and other sites occur as part of Carney complex (CNC), a multiple neoplasia syndrome caused by mutations in the PRKAR1A gene, which codes for the regulatory subunit of protein kinase A (PKA). In the present study, 17 odontogenic myxomas from patients without CNC were screened for PRKAR1A mutations and PRKAR1A protein expression by immunohistochemistry (IHC). Mutations of the coding region of the PRKAR1A gene were identified in 2 tumors; both these lesions showed no or significantly decreased immunostaining of PRKAR1A in the tumor compared to that in the surrounding normal tissue. One mutation (c.725C>A) led to a nonconservative amino acid substitution in a highly conserved area of the gene (A213D); the other was a single base-pair deletion that led to a frameshift (del774C) and a stop codon 11 amino acids downstream of the mutation site; both tumors were heterozygous for the respective mutations. Of the remaining tumors, 7 of the 15 without mutations showed almost no PRKAR1A in the tumor cells, whereas IHC showed that the protein was abundant in nontumorous cells. We concluded that PRKAR1A may be involved by its down-regulation in the pathogenesis of odontogenic myxomas caused by mutations and/or other genetic mechanisms. Of the sporadic, nonfamilial tumors associated with PRKAR1A mutations, the odontogenic type was the first myxomatous lesion found to harbor somatic PRKAR1A sequence changes.

show abstract

“…Vimentin has been detected in various mesenchymal cells types of the tooth organ, including odontoblasts (Lyn et al 1991;Seux et al 1991;Inoue et al 1992;Lombardi et al 1992a;Davidson 1994;Papagerakis et al 1999). The fact that we were unable to detect its presence in odontoblasts by high-resolution immunocytochemistry may be due to denaturation of antigenic sites during tissue processing and/or to its presence in amounts that are below the detectability threshold of Figure 1 Electron micrograph of rat incisor presecretory stage ameloblasts (PS-Am) at a position where they show short cytoplasmic processes (asterisks) extending into the forming mantle predentin.…”

Section: Discussionmentioning

confidence: 99%

Selective but Nonspecific Immunolabeling of Enamel Protein-associated Compartments by a Monoclonal Antibody Against Vimentin

Josephsen

Smith

Nanci

1999

J Histochem Cytochem.

View full text Add to dashboard Cite

SUMMARY Vimentin, an intermediate filament component, has been identified in many mesenchymal cells by a variety of LM and EM immunolabeling techniques. In our study, several tissue-processing conditions and monoclonal and polyclonal antibodies against vimentin were screened for immunostaining of rat incisor odontoblasts. Using postembedding colloidal gold immunocytochemistry, we were unable to detect any convincing vimentin antigenicity in these cells, but one of the monoclonal antibodies (V9-S) unexpectedly resulted in intense labeling over intra-and extracellular compartments that normally are strongly immunoreactive with anti-amelogenin antibodies. Blocking experiments showed that V9-S binding was competed by anti-amelogenin antibody. Immunoblots indicated that enamel proteins reacted with this anti-vimentin antibody after fixation with glutaraldehyde. These data suggest that the observed immunoreaction is directed against an epitope apparently created by crosslinking of enamel proteins during fixation. Although the labeling cannot be considered specific, it is nevertheless selective because it is very precisely localized over compartments containing enamel proteins and shows no binding to other calcified dental tissues, including dentin and bone. The V9-S antibody can therefore be used as a reliable probe to identify the presence and distribution of amelogenins in fixed tissues. (J Histochem Cytochem 47:1237-1245, 1999)

show abstract

Comparative immunohistochemical analysis between jaw myxoma and mesenchymal cells of tooth germ

Cited by 18 publications

References 17 publications

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

Distribution of Neural and Neural-Crest-Related Antigens in Human Dental Pulp by Immunohistochemistry

Molecular and immunohistochemical investigation of protein kinase a regulatory subunit type 1A (PRKAR1A) in odontogenic myxomas

Selective but Nonspecific Immunolabeling of Enamel Protein-associated Compartments by a Monoclonal Antibody Against Vimentin

Contact Info

Product

Resources

About