Comparative immunocytochemical study of release sites of insulin, glucagon and AKH-like products in Locusta migratoria, Periplaneta americana, and Carausius morosus

Raabe, Marie

doi:10.1007/bf00213931

Cited by 17 publications

(2 citation statements)

References 19 publications

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“…Moreover, medial neurosecretory cells appear to be important sites for ILP gene expression in other insect orders as well. Small clusters of dorsomedial neurosecretory cells are immunostained by ILP antisera in different life stages and species of Diptera (Duve and Thorpe, 1979;Zitnan et al, 1993;Krieger et al, 2004), Orthoptera (Raabe, 1986;Hansen et al, 1990;Claeys, 2005), Coleoptera (Sevala et al, 1993) and Hemiptera (Sevala et al, 1992). In addition, we observed ILP-like material in the Spodoptera CC-CA complex, which is known as a major storage and release site for ILPs, and in the connectives between the brain and the CC-CA complex.…”

Section: Discussionmentioning

confidence: 57%

Insulin-like peptides in Spodoptera littoralis (Lepidoptera): Detection, localization and identification

Velde

Badisco

Claeys

et al. 2007

General and Comparative Endocrinology

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Insulin is an extensively studied peptide hormone in mammals. However, insulin is not restricted to vertebrates, but has also been identified in invertebrates, among whom several insect species. These insulin-like peptides (ILPs) show structural and-at least some-functional homology with mammalian insulin and act through a conserved pathway. Yet many aspects of insulin function in insects remain to be unveiled. We analyzed the presence of ILPs in the cotton leafworm, Spodoptera littoralis, at two levels: (1) cellular localization of ILPs in whole tissues of the central nervous system from S. littoralis, and (2) detection and identification of ILPs at nucleotide level. To our knowledge, nothing about the presence of ILPs in S. littoralis has been described so far. By whole mount in situ immunolocalization, we localized bombyxin-like material in S. littoralis in four pairs of pars intercerebralis cells and in the corpus cardiacum-corpus allatum complexes. In addition, we have cloned two different S. littoralis ILP precursor cDNAs by a combination of PCR and RAcE. The corresponding precursor polypeptides ('Sl-ILPP1' and 'Sl-ILPP2') show significant sequence homology with precursors for bombyxin and other bombyxin-related peptides. Our results strongly suggest that the S. littoralis ILPs belong to the category of bombyxin-analogs.

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Section: Discussionmentioning

confidence: 57%

Insulin-like peptides in Spodoptera littoralis (Lepidoptera): Detection, localization and identification

Velde

Badisco

Claeys

et al. 2007

General and Comparative Endocrinology

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“…In insects, the nervous system appears to be the primary source of ILPs. Most commonly, small clusters of neurosecretory cells in the medial, dorsal region of brains are immunostained by insulin and ILP antisera in different life stages and species of Orthoptera (Raabe, 1986; Zachary et al ., 1988; Verhaert et al ., 1989; Hansen et al ., 1990), Hemiptera (Sevala et al ., 1992), Coleoptera (Sevala et al ., 1993), Lepidoptera (Lavenseau et al ., 1984; Mizoguchi, 1994; Nogueira et al ., 1997), and Diptera (Duve & Thorpe, 1979; El‐Salhy et al ., 1980; Gorczyca et al ., 1993; Zitnan et al ., 1993; Cao & Brown, 2001). In vertebrates, insulins originate exclusively from endocrine cells in specialized gut regions, whereas related peptides and growth factors are secreted from other tissues, including the reproductive tract.…”

Section: Introductionmentioning

confidence: 99%

Molecular characterization of insulin‐like peptide genes and their expression in the African malaria mosquito, Anopheles gambiae

Krieger

Jahan

Riehle

et al. 2004

Insect Molecular Biology

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Of the seven genes encoding insulin-like peptides (ILPs) in the mosquito, Anopheles gambiae, four are arrayed proximally as duplicate pairs on chromosome three. Amino acid substitutions encoded in the duplicate genes occur in the C peptide and not the B and A peptides. Except for one duplicated gene, sequence-specific transcripts for all other AgamILPs were obtained from female mosquitoes. Transcript expression of each AgamILP was determined by RT-PCR in the head, thorax, and abdomen of all life stages and both sexes of this mosquito. Two AgamILPs were ubiquitously expressed, suggesting a growth factor function, whereas the other AgamILPs were expressed primarily in heads, as confirmed by the immunostaining of ILPs in the neurosecretory cells of female brains, thus indicating a hormonal function.

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Insect Neuropeptides

Thorpe¹,

Duve²

1988

Current Topics in Neuroendocrinology

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Comparative immunocytochemical study of release sites of insulin, glucagon and AKH-like products in Locusta migratoria, Periplaneta americana, and Carausius morosus

Cited by 17 publications

References 19 publications

Insulin-like peptides in Spodoptera littoralis (Lepidoptera): Detection, localization and identification

Insulin-like peptides in Spodoptera littoralis (Lepidoptera): Detection, localization and identification

Molecular characterization of insulin‐like peptide genes and their expression in the African malaria mosquito, Anopheles gambiae

Insect Neuropeptides

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