Comparative IFN‐τ Secretion after Hatching by Bovine Blastocysts Derived Ex Vivo and Completely Produced In Vitro

Neira, J.A.; Tainturier, D.; L'Haridon, RM; Martal, J.

doi:10.1111/j.1439-0531.2006.00732.x

Cited by 16 publications

(10 citation statements)

References 59 publications

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“…IFNT is a biomarker of embryo viability (Hernandez-Ledezma et al, 1992;Stojkovic et al, 1999;Neira et al, 2007). Production of IFNT is related to changes in glucose metabolism and the silencing of one of the two active -chromosomes (De La Fuente et al, 1999;Bermejo-Alvarez et al, 2011b).…”

Section: Discussionmentioning

confidence: 99%

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

Green

Harvey

Spate

et al. 2015

Molecular Reproduction Devel

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The preimplantation bovine embryo displays sexual dimorphism in glucose sensitivity and interferon-tau (IFNT) secretion that are negated by inhibition of the pentose phosphate pathway, suggesting that the association between glucose metabolism and IFNT likely underpins the selective loss of female embryos. The aim of this study was to determine if altered glucose metabolism, through glucose supplementation and/or uncoupling oxidative phosphorylation with 2,4-dinitrophenol (DNP), affected embryo development. Bovine blastocyst development, sex, and IFNT production were examined in embryos cultured in the presence or absence of glucose (0, 1.5, 4 mM) with or without exposure to DNP (0, 10, 100 μM) between Days 5 and 8 post-fertilization. The absence or presence of high (4 mM) glucose reduced blastocyst development and favored the development of male embryos (P < 0.001). DNP at 10 μM had no effect, whereas 100 μM had a negative impact on blastocyst development. Notably, in the presence or even absence of glucose, supplementation with 10 μM DNP further skewed the sex ratio toward males (P < 0.05). Sexually dimorphic IFNT production was maintained in these conditions, although total production was reduced in the presence of high glucose and DNP, irrespective of embryo sex. These data suggest that the pentose phosphate pathway can modulate embryonic sex ratio and development. Therefore, bovine embryo culture should be undertaken in a low glucose (<2.5 mM) medium to minimize potential embryonic stress, as higher concentrations have sexually dimorphic effects on development and an embryo's ability to signal to the maternal reproductive tract.

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Section: Discussionmentioning

confidence: 99%

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

Green

Harvey

Spate

et al. 2015

Molecular Reproduction Devel

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“…In cattle, IFNT mRNA and IFNT protein is first detected as the trophectoderm forms at the late morula to early blastocyst stage of development (Farin et al 1989, Farin et al 1990, Hernandez-Ledezma et al 1992), but both mRNA and protein levels are very low in blastocysts (Kubisch et al 1998, Kubisch et al 2001). In vivo or in vitro derived hatched day 7 bovine blastocysts produce very low amounts of IFNT (∼100 to 1000 pmol per day) as measured by antiviral cell protection assay (Kubisch et al 1998, Neira et al 2007). Both IFNT mRNA levels in the conceptus and detectable IFNT bioactivity, determined by antiviral assay, in the uterine flush increases substantially after day 14 of pregnancy in cattle as the conceptus grows and elongates (Short et al 1991, Ealy et al 2001, Robinson et al 2006, Groebner et al 2010).…”

Section: Discussionmentioning

confidence: 99%

Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants

Spencer¹,

Forde²,

Dorniak³

et al. 2013

Reproduction

106

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In cattle, the blastocyst hatches from the zona pellucida on days 8 to 9 and then forms a conceptus that grows and elongates into an ovoid and then filamentous shape between days 9 and 16. The growing conceptus synthesizes and secretes prostaglandins and interferon tau. Our hypothesis was that the ovoid conceptus exerts a local effect on the endometrium prior to maternal recognition of pregnancy on day 16 in cattle. In Study One, synchronized cyclic heifers received nothing or 20 in vitro produced blastocysts on day 7, and uteri were collected on day 13. Interferon tau was not detected by radioimmunoassay in the uterine flush of pregnant heifers containing multiple ovoid conceptuses; however, total prostaglandin levels were higher in the uterine lumen of pregnant as compared to cyclic heifers. Microarray analysis revealed that 44 genes were increased in the endometrium of day 13 pregnant as compared to cyclic heifers, and many of those genes were classical Type I IFN-stimulated genes (ISGs). Studies Two and Three determined effects of infusing prostaglandins at the levels produced by the elongating day 14 conceptus into the uterine lumen of cyclic ewes on ISG expression in the endometrium. Results indicated that prostaglandin infusion increased the abundance of several ISGs in the endometrium. These studies support the hypothesis that the day 13 conceptus secretes prostaglandins that act locally in a paracrine manner to alter gene expression in the endometrium prior to pregnancy recognition in cattle.

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“…However, some researches also reported that the embryos completely produced in vitro secreted more IFN‐τ than the embryos produced in vivo (Wrenzycki et al. 1999; Neira et al. 2007).…”

Section: Discussionmentioning

confidence: 99%

Expression of Interferon‐tau mRNA in Bovine Embryos Derived from Different Procedures

et al. 2009

Reprod Domestic Animals

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Interferon-tau (IFN-tau) is a secreted conceptus protein which plays a critical role in the establishment of ruminant pregnancy by its antiluteolytic and antiviral effects. In the present study, we hypothesized that IFN-tau expression was temporally and spatially regulated in different pre-implantation embryos and the levels of IFN-tau expression were different among bovine embryos derived from parthenogenetic activation (PA), in vitro fertilization (IVF) and somatic cell nuclear transfer (SCNT). By using in situ hybridization with Digoxingenin (DIG)-labelled IFN-tau cDNA as a probe, we detected IFN-tau mRNA in bovine embryos from days 3 to 9 in culture. However, the timing of the initiation of IFN-tau mRNA expression was different among PA, IVF and SCNT embryos. Interferon-tau mRNA was first expressed in 16-cell stage IVF embryos on day 4, in SCNT morula on day 5 and early PA blastocyst on day 6. Semi-quantitative RT-PCR analysis showed that the expression levels of IFN-tau mRNA did not differ significantly among IVF, SCNT and PA embryos on day 7. In addition, freezing and thawing did not have a major impact either on IFN-tau mRNA expression in IVF or in vivo-produced bovine blastocysts.

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Comparative IFN‐τ Secretion after Hatching by Bovine Blastocysts Derived Ex Vivo and Completely Produced In Vitro

Cited by 16 publications

References 59 publications

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

The effects of 2,4‐dinitrophenol and d‐glucose concentration on the development, sex ratio, and interferon‐tau (IFNT) production of bovine blastocysts

Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants

Expression of Interferon‐tau mRNA in Bovine Embryos Derived from Different Procedures

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