Comparative HPLC-DAD-ESI(+)MS Fingerprint and Quantification of Phenolic and Flavonoid Composition of Aqueous Leaf Extracts of <i>Cornus mas<i> and <i>Crataegus monogyna</i>, in Relation to Their Cardiotonic Potential

Abstract

Our study aimed to compare the phenolic and flavonoid composition of two medicinal plants from the wild flora of Romania, traditionally known to be efficient in preventing cardiotoxicity: Cornus mas and Crataegus monogyna. As most previous studies have focused on fruits, our investigations aimed to fingerprint and quantify the two classes of compounds in aqueous leaf extracts, based on HPLC-DAD-ESI(+)MS analysis. The specific HPLC-DAD fingerprint was coupled with UV spectra at 280 and 340 nm to discriminate between three subclasses of compounds: two hydroxycinnamic acids and a flavonoid glycoside. While the C. monogyna extract contained more than 98% vitexin isomers (2'-and 4'-O-rhamnoside), the C. mas extract was very complex, containing a mixture of phenolic derivatives and flavonoid glycosides. The spectral patterns of C. mas, combined with the molecular mass and specific fragmentations allowed the identification of epi-catechin, coumaric and caffeic acids and quercetin derivatives. Based on LC-MS peak area and parallel calibrations with gallic acid and rutin, the mean concentration for flavonoids in C. mas was 13 mg/100 ml, while aprox. 65 mg/100 ml in C. monogyna extracts. Using the Folin method, the total phenol content was 105 mg/100 ml in C. mas and around three times less in the C. monogyna extract, while the antioxidant activity was increased only 1.5-1.7 times in C. mas extract compared to C. monogyna. These findings suggest a higher stability and potential of flavonoids to act as antioxidants in hydrophilic environment.

Comparison among Cornelian Cherry and &lt;i&gt;Prunus cerasus&lt;/i&gt; According to Phenolic Content and Antioxidant Capacity by Three Various Methods of Extraction

“…Simonian, S conducted research about FRAP activity of cornelian cherry, and they reported 235 µmol/g FRAP activity for this fruit that is in accord of our result [24]. In this experiment FRAP activity have spectra from 180 to 190 ± 40 µmol/g, that is partially consistent with previous research.…”

Metabolite fingerprinting, pathway analyses, and bioactivity correlations for plant species belonging to the Cornaceae, Fabaceae, and Rosaceae families

“…The loading plot indicated that flavonols (kaempferol derivatives, quercetin derivatives, myricetin, and myricetin derivatives) and ellagitannins (tellimagrandin II and ellagic acid) were specifically related to the Cornaceae family. In agreement with our results, similar classes of metabolites have been reported in the leaves and fruits of Cornus mas and the sarcocarp of C. officinalis (Cao et al 2011;Badalica-Petrescu et al 2014). Moreover, the ellagic and iridoid compounds have been detected in the roots of C. capitata (Tanaka et al 2001).…”

Characterization and Quantification of Phenolic Compounds from Leaf of Agarista salicifolia

“…Literature survey has revealed that plant metabolites like phenolic compounds (simple phenols, phenolic acids, flavonoids, catechins etc.,) play an important role in many of the activities like wound healing, analgesic, anti-inflammatory, anti-oxidant, insecticidal and antimicrobial activity [26,27]. Due to the great structural variety of flavonoids and phenolic acids and their presence as complex mixtures in medicinal plants, their qualitative and quantitative phytochemical characterization requires sensitive and reliable analytical methods.…”