Comparative Histopathology of Pemphigus Foliaceus and Superficial Folliculitis in the Dog

Abstract

A comparative histopathologic study of 50 cases of pemphigus foliaceus and 47 cases of superficial folliculitis in the dog was undertaken to identify those histopathologic features important in differentiating these diseases. All cases were diagnosed by the Surgical Pathology Service of the Laboratory of Pathology of the University of Pennsylvania, School of Veterinary Medicine, between January 1986 and March 1991. These cases were followed clinically to determine the accuracy of the initial histopathologic diagnosis. Data were analyzed by Fisher's exact test for discrete data and the Mann-Whitney U-test for continuous data. A probability of < 0.05 was considered statistically significant. The following breeds were affected with pemphigus foliaceus: Akita, Brittany Spaniel, Chow Chow, Collie, Dachshund, Doberman Pinscher, English Cocker Spaniel, English Setter, Golden Retriever, Siberian Husky, Laborador Retriever, Miniature Schnauzer, Old English Sheepdog, Scottish Terrier, Chinese Shar Pei, Spitz, Shetland Sheep Dog, Weimeraner, and West Highland White Terrier. An increased risk of developing pemphigus foliaceus was noted in the Akita (OR = 37.8), English Springer Spaniel (OR = 20.7), Chow Chow (OR = 12.3), Chinese Shar Pei (OR = 7.9), and Collie (OR = 3.9). Pemphigus foliaceus had a higher average density of acantholytic cells (226 +/- 22.9) than superficial folliculitis (11.8 +/- 4.6), and acantholytic cells were 183 times more likely to be present in pemphigus foliaceus. The presence of rafts of acantholytic cells occurred in pemphigus foliaceus (23/50) more often than superficial folliculitis (1/47). Recornification and reformation of pustules were, respectively, 13.4 and 3.6 times more likely to occur in pemphigus foliaceus.(ABSTRACT TRUNCATED AT 250 WORDS)

Altered Immunohistochemical Staining for Desmoglein in Skin Biopsies in Canine Pemphigus Foliaceus

“…[5][6][7]13 The PRRSV is a member of the genus Arterivirus, family Arteriviridae, order Nidovirales, and has a positive single-stranded polyadenylated RNA molecule ap- proximately 15 kb in length that contains 8 open reading frames (ORFs). 3,20 There are 2 distinct genotypes of PRRSV: the North American and European genotypes.…”

Comparative study of direct and indirect immunofluorescence for diagnosis of canine pemphigus foliaceus

“…Group I consisted of 14 dogs with clinical and histopathological findings consistent with the diagnosis of PF, while Group II consisted of 14 dogs with generalized pustules and crusted lesions with histopathological examination confirming them to be superficial pyoderma (SP). To compose the latter group, we selected animals with pyoderma because the lesions were very similar to those seen in dogs with PF and it is one of the main PF differential diagnoses (Kuhl et al, 1994;Garrod and Chidgey, 2008).…”

“…However, the histopathological diagnosis may sometimes be inconclusive, and the diagnosis is no longer established (Kuhl et al, 1994;Garrod and Chidgey, 2008). In human medicine, the immunofluorescence techniques have emerged as useful methods for diagnosing autoimmune blistering conditions since the early 1960s and present excellent results (Beutner and Gordon, 1964;Brandsen et al, 1997).…”

Canine pemphigus folliaceus: report of five clinical case

“…Σε όλες τις περιπτώσεις επιβάλλεται η αποφυγή λήψης βιο ψιών από επιπλεγμένες ή αυτοτραυματικές αλλοιώσεις, καθώς και όταν γίνεται θεραπεία με γλυκοκορτικοειδή ή άλλα ανοσοκατασταλτικά φάρμακα, επειδή αλλάζουν την ιστοπαθολογική εικόνα ή όταν έχουν προετοιμαστεί χει ρουργικά τα σημεία της βιοψίας 1019 . Με την τήρηση των παραπάνω προϋποθέσεων μπόρεσαν να διαπιστωθούν οι χαρακτηριστικές της ΦΠ ιστολογικές αλλοιώσεις και στα 5 περιστατικά 4,26 . Σε ορισμένες περιπτώσεις και ιδιαίτερα στη φαρμακευτική μορφή, είναι δυνατό να παρατηρηθούν και αποπτωτικά κύτταρα 151727 , που όμως απουσίαζαν ακό μη και στο περιστατικό 2.…”