Comparative Histopathology and Immunohistochemistry of QX-like, Massachusetts and 793/B Serotypes of Infectious Bronchitis Virus Infection in Chickens

Benyeda, Zs.; Szeredi, Levente; Mató, Tamás; Süveges, T.; Balka, G.; Abonyi-Tóth, Zs.; Rusvai, Miklós; Palya, Vilmos

doi:10.1016/j.jcpa.2010.04.007

Cited by 61 publications

(59 citation statements)

References 30 publications

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“…The predominant histopathologic and necropsy findings observed in this study included hemorrhages and congestions in the trachea and congestion of the lungs, which are consistent with previous findings reported from the field (27) and from experimental infections (2). Kidneys were affected only in a few birds that were not vaccinated and were positive for Israeli variant-2.…”

Section: Discussionsupporting

confidence: 91%

“…Kidneys were affected only in a few birds that were not vaccinated and were positive for Israeli variant-2. QX-like AvCoV-IBVs as well as strains found in Israel and northern Iraq are known to induce or be associated with kidney disease (2,18,19); our results, from both clinical and sequence analysis, suggests that these types of IBVs are rarely present in Turkish poultry flocks.…”

Section: Discussionmentioning

confidence: 52%

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Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

et al. 2016

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SUMMARY. The avian coronavirus infectious bronchitis virus (AvCoV-IBV) is recognized as an important global pathogen because new variants are a continuous threat to the poultry industry worldwide. This study investigates the genetic origin and diversity of AvCoV-IBV by analysis of the S1 sequence derived from 49 broiler flocks and 14 layer flocks in different regions of Turkey. AvCoV-IBV RNA was detected in 41 (83.6%) broiler flocks and nine (64.2%) of the layer flocks by TaqMan real-time RT-PCR. In addition, AvCoV-IBV RNA was detected in the tracheas 27/30 (90%), lungs 31/49 (62.2%), caecal tonsils 7/22 (31.8%), and kidneys 4/49 (8.1%) of broiler flocks examined. Pathologic lesions, hemorrhages, and mononuclear infiltrations were predominantly observed in tracheas and to a lesser extent in the lungs and a few in kidneys. A phylogenetic tree based on partial S1 sequences of the detected AvCoV-IBVs (including isolates) revealed that 1) viruses detected in five broiler flocks were similar to the IBV vaccines Ma5, H120, M41; 2) viruses detected in 24 broiler flocks were similar to those previously reported from Turkey and to Israel variant-2 strains; 3) viruses detected in seven layer flocks were different from those found in any of the broiler flocks but similar to viruses previously reported from Iran, India, and China (similar to Israel variant-1 and 4/91 serotypes); and 4) that the AVCoV-IBV, Israeli variant-2 strain, found to be circulating in Turkey appears to be undergoing molecular evolution. In conclusion, genetically different AvCoV-IBV strains, including vaccine-like strains, based on their partial S1 sequence, are circulating in broiler and layer chicken flocks in Turkey and the Israeli variant-2 strain is undergoing evolution.RESUMEN. Análisis filogenético y variación del gene S1 de virus de la bronquitis infecciosa detectados en pollos y gallinas de postura en Turquía.El coronavirus aviar conocido como el virus de la bronquitis infecciosa aviar (AvCoV-IBV), es reconocido como un patógeno global importante debido a que las nuevas variantes son una amenaza continua para la industria avícola en todo el mundo. En este estudio se investigó el origen genético y la diversidad de los virus de bronquitis de bronquitis mediante el análisis de la secuencia del gene S1 de virus derivados de 49 lotes de pollos y de14 lotes de ponedoras en diferentes regiones de Turquía. Se detectó ARN del virus de la bronquitis infecciosa en 41 (83.6%) lotes de pollos y en nueve (64.2%) de los lotes de ponedoras por un método de transcripción reversa y PCR en tiempo con sondas TaqMan. Además, se detectó ARN de virus de la bronquitis infecciosa en las tráqueas 27/30 (90%), los pulmones 31/49 (62.2%), las tonsilas cecales 7/22 (31.8%), y riñones 4/49 (8.1%) de las parvadas de pollo de engorde examinadas. Se observaron lesiones patológicas, hemorragias, e infiltraciones mononucleares principalmente en las tráqueas, en menor medida en los pulmones y en algunos riñones. Un árbol filogenético basado en las secuencias parciales del gene ...

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Section: Discussionsupporting

confidence: 91%

Section: Discussionmentioning

confidence: 52%

Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

et al. 2016

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“…A study recently carried out in Australia also showed that no macroscopic lesions could be observed in the oviduct of hens inoculated with the nephropathogenic strain N1/88 (Chousalkar et al, 2010). The absence of lesions might be due to inappropriate sampling time or to site of sampling (Benyeda et al, 2010). Otherwise, it might be due to the different tropism and pathogenicity for the oviduct shown by different QX-like strains.…”

Section: Analysis Of Qx-like Ibvs Recently Isolated In China 51mentioning

confidence: 97%

Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and pathogenicity and evaluation of protection induced by Massachusetts serotype H120 vaccine against QX-like strains

Sun

Han

et al. 2011

Avian Pathology

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Seventy-eight isolates of avian infectious bronchitis virus (IBV) were obtained from different field outbreaks in China in 2009 and genotyped with 34 reference strains. Four genotypes of IBV and three new isolates were identified by phylogenetic analysis and BLAST searches of the entire S1 gene. The results showed that most IBV strains that have circulated in China in recent years belong to the genotype of QX-like strains, and that they could be grouped further into two clusters, regardless of the level of genetic variation displayed. A study of pathogenicity that used three QX-like strains*ck/CH/LSD/091003, ck/CH/LDL/091022 and ck/CH/LJL/ 090330*showed that the isolates caused the most severe lesions in the kidneys and were therefore nephropathogenic strains with various levels of virulence in specific pathogen free chickens. A vaccinationÁ challenge test that was performed using the three QX-like strains showed that the commercially available H120 vaccine did not provide sufficient protection against challenge with the QX-like isolates, as demonstrated by comparison of the clinical signs, pathological lesions and virus recovery from the trachea and kidney of unvaccinatedÁchallenged and vaccinatedÁchallenged birds.

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“…In recent years some strains of IBV have caused proventriculitis (Yu et al, 2001;Gough et al, 2008;Benyeda et al, 2010;De Wit et al, 2011a;Sun et al, 2011) and enteritis (Jones, 2010a). Field infection with 793B has been associated with enteritis (Jones, 2010a).…”

Section: Virus Replication and Spreadmentioning

confidence: 99%

An overview of infectious bronchitis virus in chickens

Awad

Chhabra

Baylis

et al. 2014

World's Poultry Science Journal

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Infectious bronchitis virus (IBV) is one of the foremost causes of economic loss within the poultry industry. IBV is a commonly occurring, economically significant pathogen of commercial chicken. Economic consequences to the poultry industry comprise mortality, growth retardation and high condemnation rates in meat-type birds. In addition, decreased egg production, reduced internal and external egg quality, and reduced hatchability have been documented in layers and breeders affecting the performance of both meat-type and egg-laying birds. Apart from this some nephro-pathogenic strains cause kidney damage. Secondary pathogens can complicate the disease resulting in increased morbidity and mortality. Being a single stranded RNA virus, IBV has an enormous capacity to change both by spontaneous mutation and by genetic recombination resulting into the emergence of new variants. Since the first isolation of virus in 1937, it has been found almost all over the world. In addition, most countries are now known to have their own indigenous IBV variants. Despite the use of currently available live and inactivated vaccines, one of the most important difficulties to control IB is related to emergence of variant strains. The following paper reviews the current status of research into IBV.

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Comparative Histopathology and Immunohistochemistry of QX-like, Massachusetts and 793/B Serotypes of Infectious Bronchitis Virus Infection in Chickens

Cited by 61 publications

References 30 publications

Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

Phylogeny and S1 Gene Variation of Infectious Bronchitis Virus Detected in Broilers and Layers in Turkey

Phylogenetic analysis of infectious bronchitis coronaviruses newly isolated in China, and pathogenicity and evaluation of protection induced by Massachusetts serotype H120 vaccine against QX-like strains

An overview of infectious bronchitis virus in chickens

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