Comparative genotype reactions to Sclerotinia sclerotiorum within breeding populations of Brassica napus and B. juncea from India and China

Barbetti, Martin J.; Banga, Shashi K.; Fu, Tingdong; Li, Y. C.; Singh, Dhiraj; Liu, S. Y.; Ge, X.; Banga, S. S.

doi:10.1007/s10681-013-1049-1

Cited by 45 publications

(44 citation statements)

References 29 publications

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“…de Bary is a necrotrophic pathogen that infects more than 400 plant species, including important oil crops such as oilseed rape, soybean and sunflower 1 2 . Sclerotinia stem rot (SSR) of oilseed rape ( Brassica napus ) caused by S. sclerotiorum is the most devastating disease of this important oil crop in Australia, North America, Europe, India and China 3 . Yield losses in oilseed Brassicas (which includes oilseed rape and mustard ( B. juncea )) vary between 5 and 100% 4 .…”

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confidence: 99%

Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus

Zhao

Yang

et al. 2016

Sci Rep

125

138

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Sclerotinia stem rot caused by Sclerotinia sclerotiorum is one of the most devastating diseases in many important crops including Brassica napus worldwide. Quantitative resistance is the only source for genetic improvement of Sclerotinia-resistance in B. napus, but the molecular basis for such a resistance is largely unknown. Here, we performed dynamic transcriptomic analyses to understand the differential defense response to S. sclerotiorum in a resistant line (R-line) and a susceptible line (S-line) of B. napus at 24, 48 and 96 h post-inoculation. Both the numbers of and fold changes in differentially expressed genes in the R-line were larger than those in the S-line. We identified 9001 relative differentially expressed genes in the R-line compared with the S-line. The differences between susceptibility and resistance were associated with the magnitude of expression changes in a set of genes involved in pathogen recognition, MAPK signaling cascade, WRKY transcription regulation, jasmonic acid/ethylene signaling pathways, and biosynthesis of defense-related protein and indolic glucosinolate. The results were supported by quantitation of defense-related enzyme activity and glucosinolate contents. Our results provide insights into the complex molecular mechanism of the defense response to S. sclerotiorum in B. napus and for development of effective strategies in Sclerotinia-resistance breeding.

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confidence: 99%

Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus

Zhao

Yang

et al. 2016

Sci Rep

125

138

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“…Clearly, resistance‐screening and breeding materials should be exposed to the wide range of pathogenic variation present in N. capsellae populations. This is particularly evident from the current study, where the phenomenon of one isolate being highly virulent on one cultivar but less virulent or avirulent on other tested cultivars, highlights a dire need for breeding of cultivars that contain isolate‐independent resistances, as is already occurring for the management of similar challenges with S. sclerotiorum in oilseed brassicas (Barbetti et al , ). If host resistance is to provide a durable basis for managing white leaf spot disease of oilseed brassicas, future changes in N. capsellae virulence patterns need to be closely monitored, as do changes in expressions of host resistance to this pathogen.…”

Section: Discussionmentioning

confidence: 72%

Geographic location and year determine virulence, and year determines genetic change, in populations of Neopseudocercosporella capsellae

2019

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White leaf spot (Neopseudocercosporella capsellae) can be severe and problematic worldwide across both horticultural and oilseed Brassicaceae, including susceptible rapeseed. In this study, 82 isolates from 2015 and 106 isolates from across Australia in 2016 were first assessed for their virulence against three different rapeseed (Brassica napus) cultivars. For both years there were significant (P < 0.001) differences. Also, there were significant (all P < 0.001) differences between isolates in each year, and between cultivars. For 2016 isolates, there were also significant differences (P < 0.001) between isolates across three different Australian states, and a significant interaction (P < 0.001) between isolates with cultivars. Of the three Australian states, isolates from Victoria were most virulent. Among tested cultivars, cv. Scoop was most susceptible. Subsequently, phylogenetic analysis of 114 of these same 2015 and 2016 isolates showed current isolates clustered separately from the majority of 2005 N. capsellae isolates collected from Western Australia a decade earlier, confirming significant genetic change within N. capsellae populations over the past decade. However, isolate clusters showed no association with geographical location. The results suggest that phylogenetic association among 2005 and 2015–2016 N. capsellae isolates is complementary with pathogenicity variations explained by geographically different N. capsellae pathogen populations. Neopseudocercosporella capsellae populations are evolving rapidly, challenging management through host resistance at a time of increasing incidence and severity of white leaf spot disease over the past decade. The outcome is well illustrated by cv. Scoop, previously resistant to 2005 isolates but moderately susceptible to 2015 and highly susceptible to 2016 isolates.

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“…Previous studies reported genetic determinants of plant QDR specific of pathogen genotypes (i.e. evidence for 'pathotypes') in the interaction of B. cinerea with A. thaliana interaction (Corwin et al, 2016) and S. sclerotiorum with Brassica napus and B. juncea (Ge et al, 2012;Barbetti et al, 2014). These pathotypes may result from the combined effect of fungal genetic determinants of latency duration and plant determinants of LDT.…”

Section: Discussionmentioning

confidence: 99%

Rapid identification of an Arabidopsis NLR gene as a candidate conferring susceptibility to Sclerotinia sclerotiorum using time‐resolved automated phenotyping

et al. 2020

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SUMMARY The broad host range necrotrophic fungus Sclerotinia sclerotiorum is a devastating pathogen of many oil and vegetable crops. Plant genes conferring complete resistance against S. sclerotiorum have not been reported. Instead, plant populations challenged by S. sclerotiorum exhibit a continuum of partial resistance designated as quantitative disease resistance (QDR). Because of their complex interplay and their small phenotypic effect, the functional characterization of QDR genes remains limited. How broad host range necrotrophic fungi manipulate plant programmed cell death is for instance largely unknown. Here, we designed a time‐resolved automated disease phenotyping pipeline enabling high‐throughput disease lesion measurement with high resolution, low footprint at low cost. We could accurately recover contrasted disease responses in several pathosystems using this system. We used our phenotyping pipeline to assess the kinetics of disease symptoms caused by seven S. sclerotiorum isolates on six A. thaliana natural accessions with unprecedented resolution. Large effect polymorphisms common to the most resistant A. thaliana accessions identified highly divergent alleles of the nucleotide‐binding site leucine‐rich repeat gene LAZ5 in the resistant accessions Rubezhnoe and Lip‐0. We show that impaired LAZ5 expression in laz5.1 mutant lines and in A. thaliana Rub natural accession correlate with enhanced QDR to S. sclerotiorum. These findings illustrate the value of time‐resolved image‐based phenotyping for unravelling the genetic bases of complex traits such as QDR. Our results suggest that S. sclerotiorum manipulates plant sphingolipid pathways guarded by LAZ5 to trigger programmed cell death and cause disease.

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Comparative genotype reactions to Sclerotinia sclerotiorum within breeding populations of Brassica napus and B. juncea from India and China

Cited by 45 publications

References 29 publications

Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus

Comparative transcriptomic analysis uncovers the complex genetic network for resistance to Sclerotinia sclerotiorum in Brassica napus

Geographic location and year determine virulence, and year determines genetic change, in populations of Neopseudocercosporella capsellae

Rapid identification of an Arabidopsis NLR gene as a candidate conferring susceptibility to Sclerotinia sclerotiorum using time‐resolved automated phenotyping

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