Comparative genoprotection ability of wild-harvested <i>vs</i>. aqua-cultured <i>Ulva rigida</i> coupled with phytochemical profiling

Marques, Ana; Ferreira, João; Abreu, Helena; Pereira, Rui; Pinto, Diana; Silva, Artur M. S.; Gaivão, Isabel; Pacheco, Mário

doi:10.1080/09670262.2020.1778796

Cited by 5 publications

(7 citation statements)

References 78 publications

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“…In past decades, developments in fish nutrition towards the identification of functional feeds and a thorough understanding of underlying mechanisms have been made; however, research in this area has been mainly focused on animal growth, feed intake, fillets’ physicochemical and sensory properties, and histological analysis of the digestive apparatus and gut microbiome [ 59 ]. In more recent years, studies of DNA and chromosomal integrity, as well antioxidant system modulation, have been performed in fish [ 60 , 61 ], highlighting the potential advances for the aquaculture sector resulting from the scientific knowledge to be developed in this context.…”

Section: Discussionmentioning

confidence: 99%

“…Considering the interference of all these factors, there may be a dual interpretation for an increase of LPO; on the one hand, it can be a consequence of a failure/overwhelm of the antioxidant system, but on the other, it can signal a trigger for the activation of the antioxidant system. In some cases, exposure to moderate levels of ROS and lipid peroxidation can stimulate an adaptive response, making the cells more resilient to future oxidative pressures [ 61 ]. This interplay between lipid peroxidation and the antioxidant system is a vital part of an integrated response to oxidative stress and the efforts to maintain/restore cellular homeostasis and tissue integrity.…”

Section: Discussionmentioning

confidence: 99%

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Exploring the Antioxidant and Genoprotective Potential of Salicornia ramosissima Incorporation in the Diet of the European Seabass (Dicentrarchus labrax)

Marçal,

Sousa,

Marques

et al. 2023

Animals

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The identification of novel feed materials as a source of functional ingredients is a topical priority in the finfish aquaculture sector. Due to the agrotechnical practices associated and phytochemical profiling, halophytes emerge as a new source of feedstuff for aquafeeds, with the potential to boost productivity and environmental sustainability. Therefore, the present study aimed to assess the potential of Salicornia ramosissima incorporation (2.5, 5, and 10%), for 2 months, in the diet of juvenile European seabass, seeking antioxidant (in the liver, gills, and blood) and genoprotective (DNA and chromosomal integrity in blood) benefits. Halophyte inclusion showed no impairments on growth performance. Moreover, a tissue-specific antioxidant improvement was apparent, namely through the GSH-related defense subsystem, but revealing multiple and complex mechanisms. A genotoxic trigger (regarded as a pro-genoprotective mechanism) was identified in the first month of supplementation. A clear protection of DNA integrity was detected in the second month, for all the supplementation levels (and the most prominent melioration at 10%). Overall, these results pointed out a functionality of S. ramosissima-supplemented diets and a promising way to improve aquaculture practices, also unraveling a complementary novel, low-value raw material, and a path to its valorization.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Exploring the Antioxidant and Genoprotective Potential of Salicornia ramosissima Incorporation in the Diet of the European Seabass (Dicentrarchus labrax)

Marçal,

Sousa,

Marques

et al. 2023

Animals

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“…Keeping in mind that DNA damage is the initiating event of carcinogenesis, the decrease in DNA damage currently observed in the transgenic supplemented group, in concomitance with the reduction in the progression of epidermal dysplastic lesions, highlights the potential of G. turuturu to reduce cancer risk in organisms under preneoplastic conditions. Keeping in mind that genoprotection involves a complex network of processes and must be regarded as a multiphasic action [52], the current comet assay data concerning wild-type animals point out the need to carry out experiments over a range of G. turuturu concentrations, which will be particularly relevant to allow result extrapolation to humans and the unequivocal recommendation of a regular consumption by healthy people.…”

Section: Discussionmentioning

confidence: 99%

The Red Seaweed Grateloupia turuturu Prevents Epidermal Dysplasia in HPV16-Transgenic Mice

et al. 2021

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The role of dietary profiles in promoting or reducing the risk of multiple types of cancer is increasingly clear, driving the search for balanced foods and nutraceuticals. The red seaweed Grateloupia turuturu has been used as human food showing a balanced nutritional profile. This study aims to test in vivo chemopreventive effects of G. turuturu against cutaneous pre-malignant lesions in transgenic mice for the human papillomavirus type 16 (HPV16). Forty-four female HPV+/− or HPV−/− mice received a standard diet or were supplemented with 10% G. turuturu for 22 consecutive days. Cutaneous lesions (ear and chest skin) were identified histologically. Complementarily, the weights and histology of internal organs as well as blood biochemical and DNA integrity parameters were also assessed. G. turuturu consistently reduced the incidence of epidermal dysplasia induced by HPV16 on both cutaneous sites. Moreover, biochemical, DNA integrity and histological analyses confirmed G. turuturu edibility as no signs of toxicity were found. Dietary supplementation with G. turuturu is an effective and safe chemopreventive strategy in this model.

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“…Mononuclear blood cells were used to perform the alkaline (pH > 13) comet assay, following the methods of Collins (2004) [25], and a system of twelve gels per slide, as adopted by Marques et al (2021) [26], was used to increase the yield. Six slides precoated with normal-melting-point agarose were used for each treatment, with three replicates per animal.…”

Section: Comet Assaymentioning

confidence: 99%

Potential Health Benefits of Fruits and Vegetables II

2023

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Comparative genoprotection ability of wild-harvested vs. aqua-cultured Ulva rigida coupled with phytochemical profiling

Cited by 5 publications

References 78 publications

Exploring the Antioxidant and Genoprotective Potential of Salicornia ramosissima Incorporation in the Diet of the European Seabass (Dicentrarchus labrax)

Exploring the Antioxidant and Genoprotective Potential of Salicornia ramosissima Incorporation in the Diet of the European Seabass (Dicentrarchus labrax)

The Red Seaweed Grateloupia turuturu Prevents Epidermal Dysplasia in HPV16-Transgenic Mice

Potential Health Benefits of Fruits and Vegetables II

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