Comparative genomics with a multidrug-resistant Klebsiella pneumoniae isolate reveals the panorama of unexplored diversity in Northeast Brazil

Profeta, Rodrigo; Seyffert, Núbia; Tiwari, Sandeep; Viana, Marcus Vinícius Canário; Jaiswal, Arun Kumar; Caetano, Ana Carolina Barbosa; Bücker, Daniel Henrique; Oliveira, Luciana Tavares de; Santos, Roselane Gonçalves dos; Gala-García, Alfonso; Kato, Rodrigo Bentes; Padilha, Francine Ferreira; Lima-Verde, I.B.; Ghosh, Preetam; Barh, Debmalya; Goés-Neto, Aristóteles; Figueiredo, Henrique César Pereira; Soares, Siomar C.; Meyer, Roberto; Brenig, Bertram; Ramos, Pablo Ivan Pereira; Azevedo, Vasco; Castro, Thiago Luiz de Paula

doi:10.1016/j.gene.2020.145386

Cited by 3 publications

(3 citation statements)

References 69 publications

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“…The bacterium used in the intratracheal pulmonary infection experiments was K. pneumoniae B31, a clinical isolate with a multidrug-resistance pro le [37], gently provided by Prof. Vasco Ariston from the Laboratory of Cellular and Molecular Genetics at ICB/UFMG. For the procedure, animals were anesthetized intraperitoneally (i.p.)…”

Section: Klebsiella Pneumoniae Intratracheal Infectionmentioning

confidence: 99%

Gut microbiota from patients with mild COVID-19 cause alterations in mice that resemble post-COVID syndrome

Almeida

Engel

Ricci

et al. 2022

Preprint

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Background There is mounting evidence that SARS-CoV-2 targets tissues beyond the respiratory tract. Long-term sequelae after COVID-19 are frequent and of major concern. Prolonged virus detection in the gut has been particularly intriguing. Of note, SARS-CoV-2 infection also disturbs the gut microbiota composition, a finding linked with disease severity in patients with COVID-19. Here, we aimed to characterize the functional role of the gut microbiota in the long-term consequences of COVID-19. To this end, we characterized the gut microbiota from COVID-19 human subjects and followed the effects of human fecal transfer to germ-free mice. Results The gut microbiota of post-COVID subjects (up to 4 months from the initial positive test) revealed a remarkable predominance of Enterobacteriaceae strains with multidrug-resistance phenotype compared to healthy controls. After fecal transfer to germ-free mice, animals receiving samples from post-COVID subjects displayed higher lung inflammation and increased susceptibility to pulmonary infection caused by an antimicrobial resistant Klebsiella pneumoniae strain. These mice also showed poorer cognitive performance associated with increased expression of TNF-α, reduced levels of brain-derived neurotrophic factor-BDNF and postsynaptic density protein-PSD-95 in the brain, as well as alterations of several biochemical pathways. These alterations were observed in the absence of SARS-CoV-2, suggesting that alterations in the gut microbiota caused them. Consistent with this hypothesis, brain dysfunctions induced in a mouse model of coronavirus infection were partially prevented by modulation of the microbiota via treatment with the commensal probiotic bacteria Bifidobacterium longum 51A. Conclusions Our results show prolonged impact of SARS-CoV-2 infection in the gut microbiota that persists even after the individuals have cleared the virus. Increased Enterobacteriaceae with antimicrobial resistance phenotype were of particular concern. Moreover, microbiota transfer from post-COVID subjects induced loss of brain cognitive functions and impaired lung defense in mice. Altogether, our work emphasizes the importance of microbiota as a target for therapies to help treat post-COVID sequelae.

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Section: Klebsiella Pneumoniae Intratracheal Infectionmentioning

confidence: 99%

Gut microbiota from patients with mild COVID-19 cause alterations in mice that resemble post-COVID syndrome

Almeida

Engel

Ricci

et al. 2022

Preprint

View full text Add to dashboard Cite

show abstract

“…K. pneumoniae B31, a clinical isolate with an AMR profile, 82 was kindly provided by Prof. Vasco Azevedo, Laboratory of Cellular and Molecular Genetics at ICB/UFMG. Intratracheal infection was induced as previously described.…”

Section: Methodsmentioning

confidence: 99%

Gut microbiota from patients with COVID-19 cause alterations in mice that resemble post-COVID symptoms

Mendes de Almeida,

Engel,

Ricci

et al. 2023

Gut Microbes

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Long-term sequelae of coronavirus disease (COVID)-19 are frequent and of major concern. Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection affects the host gut microbiota, which is linked to disease severity in patients with COVID-19. Here, we report that the gut microbiota of post-COVID subjects had a remarkable predominance of Enterobacteriaceae strains with an antibiotic-resistant phenotype compared to healthy controls. Additionally, short-chain fatty acid (SCFA) levels were reduced in feces. Fecal transplantation from post-COVID subjects to germ-free mice led to lung inflammation and worse outcomes during pulmonary infection by multidrug-resistant Klebsiella pneumoniae . transplanted mice also exhibited poor cognitive performance. Overall, we show prolonged impacts of SARS-CoV-2 infection on the gut microbiota that persist after subjects have cleared the virus. Together, these data demonstrate that the gut microbiota can directly contribute to post-COVID sequelae, suggesting that it may be a potential therapeutic target.

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“…K. pneumoniae B31, a clinical isolate with an AMR profile (Profeta et al, 2021), was kindly provided by Prof. Vasco Azevedo, Laboratory of Cellular and Molecular Genetics at ICB/UFMG. Intratracheal infection was performed as previously described (Vieira et al, 2016b).…”

Section: Klebsiella Pneumoniae Infectionmentioning

confidence: 99%

Gut microbiota from patients with COVID-19 cause alterations in mice that resemble post-COVID symptoms

Almeida

Engel

Ricci

et al. 2023

Preprint

View full text Add to dashboard Cite

Long-term sequelae after Coronavirus disease (COVID)-19 are frequent and of major concern. SARS-CoV-2 infection affects the host's gut microbiota, which is linked with disease severity in patients with COVID-19. We report here that the gut microbiota of post-COVID subjects had a remarkable predominance of Enterobacteriaceae strains with antibiotic-resistance phenotype compared to healthy controls. Additionally, short-chain fatty acids (SCFA) levels were reduced in their feces. Fecal transplant from post-COVID subjects to germ-free mice led to lung inflammation and worst outcomes during pulmonary infection by multidrug-resistant Klebsiella pneumoniae. Transplanted mice also had poorer cognitive performance. Overall, we show prolonged impacts of SARS-CoV-2 infection in the gut microbiota that persist after subjects have cleared the virus. Together, these data demonstrate that the gut microbiota can directly contribute to post-COVID sequelae, suggesting that it may be a potential therapeutic target.

show abstract

Comparative genomics with a multidrug-resistant Klebsiella pneumoniae isolate reveals the panorama of unexplored diversity in Northeast Brazil

Cited by 3 publications

References 69 publications

Gut microbiota from patients with mild COVID-19 cause alterations in mice that resemble post-COVID syndrome

Gut microbiota from patients with mild COVID-19 cause alterations in mice that resemble post-COVID syndrome

Gut microbiota from patients with COVID-19 cause alterations in mice that resemble post-COVID symptoms

Gut microbiota from patients with COVID-19 cause alterations in mice that resemble post-COVID symptoms

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