Comparative Genomics Uncovers the Genetic Diversity and Synthetic Biology of Secondary Metabolite Production of<i>Trametes</i>

Zhang, Yan; Wang, Jingjing; Chen, Yajun; Zhou, Minghui; Wang, Wei; Geng, Meiyu; Xu, Deyang; Xu, Zhongdong

doi:10.1080/12298093.2020.1725361

Cited by 8 publications

(4 citation statements)

References 52 publications

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“…Investigations are ongoing to identify the sources of their anti-mycobacterial activity and to determine whether any may be due to the production of novel bioactive compounds. Species of Lophodermium and Pseudaegerita have previously been found to produce several antifungal compounds ( Hosoya et al, 2007 ; Sumarah et al, 2011 ; McMullin et al, 2015 ), while T. coccinea is predicted to have secondary metabolite pathways though genomic analysis ( Zhang et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

et al. 2021

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There is a real and urgent need for new antibiotics able to kill Mycobacteria, acid-fast bacilli capable of causing multiple deadly diseases. These include members of the Mycobacterium tuberculosis complex, which causes the lung disease tuberculosis (TB) as well as non-tuberculous Mycobacteria (NTM) a growing cause of lung, skin, soft tissue, and other infections. Here we describe a medium-throughput bioluminescence-based pipeline to screen fungi for activity against Mycobacteria using the NTM species Mycobacterium abscessus and Mycobacterium marinum. We used this pipeline to screen 36 diverse fungal isolates from the International Collection of Microorganisms from Plants (ICMP) grown on a wide variety of nutrient-rich and nutrient-poor media and discovered that almost all the tested isolates produced considerable anti-mycobacterial activity. Our data also provides strong statistical evidence for the impact of growth media on antibacterial activity. Chemical extraction and fractionation of a subset of the ICMP isolates revealed that much of the activity we observed may be due to the production of the known anti-mycobacterial compound linoleic acid. However, we have identified several ICMP isolates that retained their anti-mycobacterial activity in non-linoleic acid containing fractions. These include isolates of Lophodermium culmigenum, Pseudaegerita viridis, and Trametes coccinea, as well as an unknown species of Boeremia and an isolate of an unknown genus and species in the family Phanerochaetaceae. Investigations are ongoing to identify the sources of their anti-mycobacterial activity and to determine whether any may be due to the production of novel bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

et al. 2021

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“…Comparative genomics provides an important technical means for studying the origin and maintenance of fungal genetic diversity (Ma et al 2013 ; de Vries et al 2017 ; Kiss et al 2019 ; Zhang et al 2020 ). However, the genome structure and genetic diversity of coprinoid mushroom species have not been extensively studied.…”

Section: Introductionmentioning

confidence: 99%

Comparative genomic analysis of five coprinoid mushrooms species

Wang

Zhang

Ding

et al. 2023

Funct Integr Genomics

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Although coprinoid mushrooms are widely known for the phenomenon of deliquescence and production of fungal laccases and extracellular peroxygenases, the genome structure and genetic diversity of coprinoid mushroom species have not been extensively studied. To reveal the genomic structure and diversity in coprinoid mushroom species, the genomes of five coprinoid mushroom species were compared and analyzed. A total of 24,303 orthologous gene families, including 89,462 genes, were identified in the five species. The numbers of core, softcore, dispensable, and private genes were 5617 (25.6%), 1628 (7.4%), 2083 (9.5%), and 12,574 (57.4%), respectively. Differentiation time analysis revealed that Coprinellus micaceus and Coprinellus angulatus differentiated approximately 181.0 million years ago. Coprinopsis cinerea and Coprinopsis marcescibilis differentiated approximately 131.0 million years ago, and they were differentiated from Candolleomyces aberdarensis approximately 176.0 million years ago. Gene family contraction and expansion analyses showed that 1465 genes and 532 gene families were expanded, and 95 genes and 134 gene families were contracted. Ninety-five laccase-coding genes were detected in the five species, and the distribution of the laccase-coding genes in the five species was not uniform. These data provide a reference for a deeper understanding of the genetic structure of the genomes of coprinoid mushroom species. Furthermore, this study provides a reference for follow-up studies on the genome structure of coprinoid mushroom species and the diversity of specific functional genes.

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“…For instance, ganodermin and rufuslactone have been found to show potent antifungal properties (Luo et al 2005;Wang and Ng 2006). Other bioactive metabolites from higher fungi includes ganoderic acid T, ganoderic acid F (antimetastatic effect), ganoderic acid Me (Anti-metastatic effect, reduce tumor invasion), ganoderic acid DM (anti-proliferative effect), lucidenic acid N, lucialdehyde B, lucialdehyde C (anticancer activity), 11β-Hydroxy-3,7-dioxo-5α-lanosta8,24(E)-dien-26-oic acid (Cytotoxic effect), Cortinellin (antibacterial, antifungal), sesquiterpenoid and hydroquinones (antibacterial activity), transcinnamic acid, photocattheuric acid, caffeic acid, gallic acid, ascorbic acid and benzoic acid (antioxidant activity) (Herrmann 1962;Wu et al 2001Wu et al , 2012Gao et al 2002 Trametes coccinea (also known as Pycnoporus coccineus), is one of the white-rot fungus (Basidiomycetes) belonging to the family Polyporaceae and the class Agaricomycetes of fungi, which most commonly grows in the outer layer of decaying woods and also found on the surface of deciduous trees (Zhang et al 2020). This mushroom produces brightred colour pigments, which is deposited in the fruiting bodies.…”

Section: Introductionmentioning

confidence: 99%

Cinnabarinic acid from Trametes coccinea fruiting bodies exhibits antibacterial activity through inhibiting the biofilm formation

et al. 2022

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Wild mushrooms are rich sources of natural compounds with potent bioactive properties. Several important metabolites have been reported from mushrooms, which possess clinically important bioactive properties like antibacterial activity, anticancer activity, antidiabetic activity and antioxidant activity. In this study, we have evaluated the antimicrobial activity of Trametes coccinea fruiting body extracts against different bacterial isolates viz. Bacillus subtilis, Bacillus cereus and Escherichia coli. Fruiting bodies of three T. coccinea samples, of which two were collected from Santipur, Arunachal Pradesh and one collected from Jorhat, Assam, were used for extraction using methanol. The extracts showed potent antimicrobial activity against all the test bacteria. Minimum Inhibitory Concentration (MIC) of the extracts against Bacillus subtilis, Bacillus cereus and Escherichia coli was recorded as 400 µg/ml, 400 µg/ml and 300 µg/ml respectively. Further, the bioactive compounds of the extract were separated and detected using Thin Layer Chromatography (TLC). Presence of Cinnabarinic acid (CBA), a potent antimicrobial compound was detected in TLC, which was further con rmed through High Performance Liquid Chromatography (HPLC) and Electrospray Ionization-Mass Spectrometry (ESI-MS). Cinnabarinic acid was able to inhibit the formation of bio lms in Bacillus subtilis and B. cereus, suggesting that the compound can be bene cial in the management of bio lm based antimicrobial resistance.investigations will surely unveil the molecular mechanism of bio lm inhibition by identifying the potential targets of cinnabarinic acid in bacterial cells.

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Comparative Genomics Uncovers the Genetic Diversity and Synthetic Biology of Secondary Metabolite Production ofTrametes

Cited by 8 publications

References 52 publications

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

Screening of Fungi for Antimycobacterial Activity Using a Medium-Throughput Bioluminescence-Based Assay

Comparative genomic analysis of five coprinoid mushrooms species

Cinnabarinic acid from Trametes coccinea fruiting bodies exhibits antibacterial activity through inhibiting the biofilm formation

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