Comparative genomics uncovers genetic diversity and synthetic biology of secondary metabolite production of the genus Trametes

Abstract

It’s well-established that the CAZyme genes of genus Trametes contributed to the degradation processes of polysaccharides, including lignin or crystalline cellulose. However, the comprehensive analysis of the composition of CAZymes and the biosynthetic gene clusters of Trametes genus remain unclear. We conducted comparative analysis, detected the CAZyme genes, and predicted the biosynthetic gene clusters for 9 Trametes strains. Among 82,053 homologous clusters we obtained for genus Trametes, we identified 8,518 core genes, 60,441 accessory genes and 13,094 specific genes. Our results showed that a large proportion of CAZyme genes were catalogued into glycoside hydrolases, glycosyltransferases, and carbohydrate esterases. The predicted BGCs of Trametes genus were divided into 6 strategies and the 9 Trametes strains harbored 47.78 BGCs on average. Our study uncovers the genus Trametes exhibited an open pan-genome structure, provides insights into the genetic diversity and explores the synthetic biology of secondary metabolite production for Trametes genus.

In Vitro Antimicrobial Activity of Cinnabarin on Xanthomonas campestris Isolated from Bean Crops of Puebla, Mexico

“…This anthraquinone exhibits antimicrobial activity against Bacillus cereus, Enterococcus faecalis, Enteroccocus faecium, Escherichia coli, Klebsiela pneumoniae, Lissteria measureneroides, Lactobacillus plantarum, Pseudomonas aeruginosa, Salmonella sp. and Staphylococcus aureus [11]. The production of this antibiotic occurs at days 18-22 of fungal growth.…”