2014
DOI: 10.1016/j.mimet.2014.03.009
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Comparative genomics study for the identification of drug and vaccine targets in Staphylococcus aureus: MurA ligase enzyme as a proposed candidate

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Cited by 12 publications
(6 citation statements)
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“…In the modern postgenomic period, the probabilities of selecting suitable targets through computational methods and integration of "omics" data, such as proteomics, metabolomics, and genomics have been expanding continuously. The in silico approaches such as subtractive and comparative proteomics are now being used extensively for the identification as well as the prediction of drug targets for several pathogenic bacteria [13]. Compared to traditional methods, these techniques are efficient, time saving as well as cost effective in drug designing processes [14].…”
Section: Introductionmentioning
confidence: 99%
“…In the modern postgenomic period, the probabilities of selecting suitable targets through computational methods and integration of "omics" data, such as proteomics, metabolomics, and genomics have been expanding continuously. The in silico approaches such as subtractive and comparative proteomics are now being used extensively for the identification as well as the prediction of drug targets for several pathogenic bacteria [13]. Compared to traditional methods, these techniques are efficient, time saving as well as cost effective in drug designing processes [14].…”
Section: Introductionmentioning
confidence: 99%
“…That is the reason we set out in this study a systematic approach to identify and annotate prokaryotic ortholog gene circuits whose presence or absence are linked to phenotypes of antagonism to the host. Comparative genomics has been utilized before in the identification of drug and vaccine targets in Staphylococcus aureus [ 22 ] and mycobacterial peptidoglycan remodeling enzymes linked to pathogenicity [ 23 ]. Ours, however, takes it one step further, employing comparisons among 2527 distinct genomes.…”
Section: Introductionmentioning
confidence: 99%
“…The collected protein sequences of unique pathways of the pathogen from the above step were subjected to NCBI BLASTP search with an e-value threshold set to 0.005 against human proteome. Proteins showing ‘no significant similarities’ or ‘no hits’ were considered as non-homologous proteins to the host proteome (Anishetty et al 2005; Ghosh et al 2014). Since non-homologous proteins minimize the chances of side effects and cross-reactivity caused by antibiotics, hence they can effectively serve as therapeutic candidates.…”
Section: Methodsmentioning
confidence: 99%