Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3

Haan, Caroline P. A. Skarp-de; Culebro, Alejandra; Schott, Thomas; Revez, Joana; Schweda, Elke K. H.; Hänninen, Marja‐Liisa; Rossi, Mirko

doi:10.1186/1471-2164-15-129

Cited by 39 publications

(78 citation statements)

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“…In total, 144 C. coli isolates, including 90 isolates from swine, 34 from humans, 18 from poultry, and 2 from wild birds, were chosen for LOS locus screening. The selection comprised 133 C. coli isolates collected between 1996 and 2012 from Finnish human patients, chickens, and pigs reared in Finland and wild birds sampled in the Helsinki region in previous studies (25,(33)(34)(35)(36)(37)(38)(39). This collection was supplemented with 11 C. coli isolates from the Campynet (CNET) collection (hosted by DSMZ GmbH [https: //www.dsmz.de/]).…”

Section: Methodsmentioning

confidence: 99%

“…Isolate selection was based on genotype (pulsed-field gel electrophoresis [PFGE] or multilocus sequence typing [MLST]), host, country of origin, and year of isolation to encompass the greatest possible diversity. Cultivation and DNA isolation were carried out as previously described (25), unless otherwise stated.…”

Section: Methodsmentioning

confidence: 99%

“…The size of the LOS locus was estimated by gel electrophoresis with 1-kbplus (Thermo Scientific) and long-range (Thermo Scientific) molecular weight markers. Specific primers for each class were designed based on the previously described C. coli LOS locus classes (classes I to IX) (25,26). Primer pairs and their amplicon sizes for each LOS class are shown in Table 1, and a graphic representation of the primer annealing positions within the LOS locus is shown in Fig.…”

Section: Methodsmentioning

confidence: 99%

“…However, so far, only two studies have addressed the variation in gene composition of the C. coli LOS biosynthesis locus. Until now, nine genetic classes composed of a variable combination of 10 to 20 genes have been described in C. coli (25,26), but no chemical analysis of their LOS structures was executed. A couple of decades ago, the LOS struc-ture of a single C. coli strain was described (27).…”

mentioning

confidence: 99%

“…We expanded the current C. coli LOS classification by describing three additional LOS locus classes (25,26). Moreover, by analyzing genomic data with the LOS chemical composition of selected isolates, we identified possible associations between gene content in the LOS biosynthesis locus and observed differences in LOS phenotype.…”

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confidence: 99%

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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

et al. 2016

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Despite the importance of lipooligosaccharides (LOSs) in the pathogenicity of campylobacteriosis, little is known about the genetic and phenotypic diversity of LOS in Campylobacter coli. In this study, we investigated the distribution of LOS locus classes among a large collection of unrelated C. coli isolates sampled from several different host species. Furthermore, we paired C. coli genomic information and LOS chemical composition for the first time to investigate possible associations between LOS locus class sequence diversity and biochemical heterogeneity. After identifying three new LOS locus classes, only 85% of the 144 isolates tested were assigned to a class, suggesting higher genetic diversity than previously thought. This genetic diversity is at the basis of a completely unexplored LOS structural heterogeneity. Mass spectrometry analysis of the LOSs of nine isolates, representing four different LOS classes, identified two features distinguishing C. coli LOS from that of Campylobacter jejuni. 2-Amino-2-deoxy-D-glucose (GlcN)-GlcN disaccharides were present in the lipid A backbone, in contrast to the ␤-1=-6-linked 3-diamino-2,3-dideoxy-D-glucopyranose (GlcN3N)-GlcN backbone observed in C. jejuni. Moreover, despite the fact that many of the genes putatively involved in 3-acylamino-3,6-dideoxy-D-glucose (Quip3NAcyl) were apparently absent from the genomes of various isolates, this rare sugar was found in the outer core of all C. coli isolates. Therefore, regardless of the high genetic diversity of the LOS biosynthesis locus in C. coli, we identified species-specific phenotypic features of C. coli LOS that might explain differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. IMPORTANCEDespite the importance of C. coli to human health and its controversial role as a causative agent of Guillain-Barré syndrome, little is known about the genetic and phenotypic diversity of C. coli LOSs. Therefore, we paired C. coli genomic information and LOS chemical composition for the first time to address this paucity of information. We identified two species-specific phenotypic features of C. coli LOS, which might contribute to elucidating the reasons behind the differences between C. jejuni and C. coli in terms of population dynamics and host adaptation. C ampylobacteriosis is the most common bacterial foodborne disease in developed countries, with over 200,000 human cases being reported annually in the European Union alone (1). The true burden of the disease in the population is likely underestimated, as many infections result in mild gastroenteritis (1). Approximately 80% of reported infections are caused by Campylobacter jejuni, and 7 to 18% of cases are attributed to C. coli. Therefore, C. coli is among the five most important bacterial etiological agents of human gastroenteritis (2, 3).As in other Gram-negative bacteria, Campylobacter species cell surface glycoconjugates, including lipooligosaccharides (LOSs), play an important role in serum and bile resistance; resistanc...

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

et al. 2016

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Characterization of Swedish Campylobacter coli clade 2 and clade 3 water isolates

et al. 2018

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Campylobacter jejuni and Campylobacter coli are important bacterial enteropathogens. Poultry is the best‐known reservoir for Campylobacter infection but natural bodies of water have also been shown to be important pathways for transmission. Campylobacter can survive in cold water but most of the studies have focused on C. jejuni only. In this paper, we take a closer look at the biology and water survival strategies of C. coli. Eight C. coli isolates cultivated from raw (incoming) surface water at water plants in Sweden were characterized using whole‐genome sequencing and phenotypical assays. Phylogenetic analysis assigned the Swedish water isolates to clades 2 and 3, known to include C. coli of environmental origin. In addition, 53 earlier published sequences of C. coli clade 2 and 3 from environmental waters were included for in silico analyses. Generally, clade 2 isolates had larger genomes, which included a functional tricarballylate utilization locus, while clade 3 isolates contained different genes involved in oxidative stress as well as putative virulence factors. The Swedish water isolates of clade 2 formed large, blurry bacterial colonies on agar, whereas clade 3 colonies were smaller. All Swedish isolates were motile, but clade 3 isolates formed larger motility zones on soft agar, and none of these isolates produced biofilm. Although water survival varied between the analyzed isolates, there were hardly any clade‐specific significant differences. Our results highlight the diversity of C. coli in general, and show differences in metabolic capabilities and ways to handle oxidative stress between clade 2 and 3 water isolates.

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Population Biology and Comparative Genomics of Campylobacter Species

Epping

Antão

Semmler

2021

Current Topics in Microbiology and Immunology

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The zoonotic pathogen Campylobacter is the leading cause for bacterial foodborne infections in humans. Campylobacters are most commonly transmitted via the consumption of undercooked poultry meat or raw milk products. The decreasing costs of whole genome sequencing enabled large genome-based analyses of the evolution and population structure of this pathogen, as well as the development of novel high-throughput molecular typing methods. Here, we review the evolutionary development and the population diversity of the two most clinically relevant Campylobacter species; C. jejuni and C. coli. The state-of-the-art phylogenetic studies showed clustering of C. jejuni lineages into host specialists and generalists with coexisting lifestyles in chicken and livestock-associated hosts, as well as the separation of C. coli isolates of riparian origin (waterfowl, water) from C. coli isolated from clinical and farm-related samples. We will give an overview of recombination between both species and the potential impact of horizontal gene transfer on host adaptation in Campylobacter. Additionally, this review briefly places the current knowledge of the population structure of other Campylobacter species such as C. lari, C. concisus and C. upsaliensis into perspective. We also provide an overview of how molecular typing methods such as multilocus sequence typing (MLST) and whole genome MLST have been used to detect and trace Campylobacter outbreaks along the food chain.

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Comparative genomics of unintrogressed Campylobacter coli clades 2 and 3

Cited by 39 publications

References 70 publications

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Large Sequence Diversity within the Biosynthesis Locus and Common Biochemical Features of Campylobacter coli Lipooligosaccharides

Characterization of Swedish Campylobacter coli clade 2 and clade 3 water isolates

Population Biology and Comparative Genomics of Campylobacter Species

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