Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient

Phuc, Bui Hoang; Tuan, Vo Phuoc; Binh, Tran Thanh; Tung, Pham Huu; Tri, Tran Dinh; Dung, Ho Dang Quy; Thuan, Ngo Phuong Minh; Fauzia, Kartika Afrida; Tshibangu‐Kabamba, Evariste; Alfaray, Ricky Indra; Saruuljavkhlan, Batsaikhan; Matsumoto, Takashi; Akada, Junko; Yamaoka, Yoshio

doi:10.1038/s41598-023-35527-4

Cited by 2 publications

(3 citation statements)

References 70 publications

(83 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…pylori strains, HP22 and HP34, isolated in central Vietnam, were added to a collection of 85 H. pylori isolates recently reported from southern Vietnam [33, 34]. Collectively, the majority of H.…”

Section: Resultsmentioning

confidence: 99%

“…In this study, the genome data for two H. pylori strains, HP22 and HP34, isolated in central Vietnam, were added to a collection of 85 H. pylori isolates recently reported from southern Vietnam [33,34]. Collectively, the majority of H. pylori isolates in Vietnam (80.5 %, 70/87) belonged to the hpEastAsia population, while the remaining isolates belonged to the hpEurope population (19.5 %, 17/87).…”

Section: Genomic Comparison Of H Pylori Strains In Vietnam and Southe...mentioning

confidence: 99%

“…Among the isolates, 70 (70/87) were found to be cagA-positive. The most frequent genotypes among the cagA-positive isolates were vacA s1m1/cagA+ and vacA s1m2/cagA+, accounting for 51.4 (36/70) and 48.6 % (34/70) of the isolates, respectively [33,34].…”

Section: Genomic Comparison Of H Pylori Strains In Vietnam and Southe...mentioning

confidence: 99%

See 2 more Smart Citations

Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia

Nguyen,

Do,

Nguyen

et al. 2024

Journal of Medical Microbiology

View full text Add to dashboard Cite

Introduction. Helicobacter pylori infection is a major global health concern, linked to the development of various gastrointestinal diseases, including gastric cancer. To study the pathogenesis of H. pylori and develop effective intervention strategies, appropriate animal pathogen models that closely mimic human infection are essential. Gap statement. This study focuses on the understudied hpEastAsia genotype in Southeast Asia, a region marked by a high H. pylori infection rate. No mouse-adapted model strains has been reported previously. Moreover, it recognizes the urgent requirement for vaccines in developing countries, where overuse of antimicrobials is fuelling the emergence of resistance. Aim. This study aims to establish a novel mouse-adapted H. pylori model specific to the hpEastAsia genotype prevalent in Southeast Asia, focusing on comparative genomic and histopathological analysis of pathogens coupled with vaccine preclinical studies. Methodology. We collected and sequenced the whole genome of clinical strains of H. pylori from infected patients in Vietnam and performed comparative genomic analyses of H. pylori strains in Southeast Asia. In parallel, we conducted preclinical studies to assess the pathogenicity of the mouse-adapted H. pylori strain and the protective effect of a new spore-vectored vaccine candidate on male Mlac:ICR mice and the host immune response in a female C57BL/6 mouse model. Results. Genome sequencing and comparison revealed unique and common genetic signatures, antimicrobial resistance genes and virulence factors in strains HP22 and HP34; and supported clarithromycin-resistant HP34 as a representation of the hpEastAsia genotype in Vietnam and Southeast Asia. HP34-infected mice exhibited gastric inflammation, epithelial erosion and dysplastic changes that closely resembled the pathology observed in human H. pylori infection. Furthermore, comprehensive immunological characterization demonstrated a robust host immune response, including both mucosal and systemic immune responses. Oral vaccination with candidate vaccine formulations elicited a significant reduction in bacterial colonization in the model. Conclusion. Our findings demonstrate the successful development of a novel mouse-adapted H. pylori model for the hpEastAsia genotype in Vietnam and Southeast Asia. Our research highlights the distinctive genotype and pathogenicity of clinical H. pylori strains in the region, laying the foundation for targeted interventions to address this global health burden.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Genomic Comparison Of H Pylori Strains In Vietnam and Southe...mentioning

confidence: 99%

See 1 more Smart Citation

Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia

Nguyen,

Do,

Nguyen

et al. 2024

Journal of Medical Microbiology

View full text Add to dashboard Cite

show abstract

A comparative whole genome comparison analysis of Helicobacter pylori from gastric cancer and gastritis in China setting

Kong,

Yan,

Duan

et al. 2024

Preprint

View full text Add to dashboard Cite

Background: This study aimed to explore and compare the differences in the genomics and pathogenicity of Helicobacter pylori (H. pylori) strains derived from the gastric cancer (GC) and gastritis (GI) in the Chinese population. Methods: We sequenced 12 H. pylori from GC and GI patients in china by whole genome sequencing. 20 H. pylori sequencing data from other regions of the world were obtained from the public platform as reference genes. Then, the evolutionary tree was drawn based on multi-omics, and the differences of virulence factors (VF) and gene function were analyzed Results: In GC stains, the 1544-1640 coding genes, with a total length of 1,549,790-1,605,249 bp, were predicted. In GI stains, the 1552-1668 coding genes, with a total length of 1,552,426-1,665,981 bp, were identified. In addition, the average length of coding genes in GC and GI strain, was approximately 1594 (90.91%) and 1589 genes (90.81%), respectively. We found that the VFs predicted by the two cohort strains had high consistency, but their cagA status was significantly different. Additionally, the clustering results indicated that there were significant differences in core Single Nucleotide Polymorphism (SNP) between GC and GI strains, but no significant differences in homologous proteins and gene island prediction between the two strains. Subsequently, the results of pan-genomic and Average Nucleotide Identity (ANI) analyses suggested that GC, GI and other reference H. pylori strains had high homology consistency. Furthermore, the gene function annotation results suggested that the H. pylori strains of GC and GI also had high similarity in gene function, and their specific gene functions were mainly concentrated in the process of metabolism, transcription and repair. Conclusions: GC and GI patient-derived H. pylori have some differences in VF and SNP, but they also have high homologous consistency at other level of the genome in Chinese population.

show abstract

Comparative genomics of two Vietnamese Helicobacter pylori strains, CHC155 from a non-cardia gastric cancer patient and VN1291 from a duodenal ulcer patient

Cited by 2 publications

References 70 publications

Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia

Genomic and vaccine preclinical studies reveal a novel mouse-adapted Helicobacter pylori model for the hpEastAsia genotype in Southeast Asia

A comparative whole genome comparison analysis of Helicobacter pylori from gastric cancer and gastritis in China setting

Contact Info

Product

Resources

About