Comparative genomics of Thermus thermophilus: Plasticity of the megaplasmid and its contribution to a thermophilic lifestyle

Brüggemann, Holger; Chen, Chao-Yin

doi:10.1016/j.jbiotec.2006.03.043

Cited by 65 publications

(62 citation statements)

References 32 publications

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“…The remaining part of the sequence (two-thirds of the protein), hosting the second heme-binding site, showed ϳ50% sequence identity (Fig. 1B) with the well-characterized cytochrome c 552 from T. thermophilus, the substrate of ba 3 and caa 3 cytochrome c oxidases (21,35,37), the two respiratory terminal oxidases of this organism (11,40).…”

Section: Resultsmentioning

confidence: 96%

“…In bacteria and archaea, they can also utilize sulfur compounds as an electron source for chemotrophic, lithotrophic, and phototrophic growth (13,32). SORs catalyze the two-electron oxidation of sulfite to sulfate (SO 3 2Ϫ ϩ H 2 O 3 SO 4 2Ϫ ϩ 2 H ϩ ϩ 2 e Ϫ ), where the final electron acceptor is O 2 and/or heme iron ions (26). Most SORs possess a heme relay, shuttling electrons from the molybdenum cofactor to the subsequent acceptor, while in plants, for instance in Arabidopsis thaliana, no heme relay is present and O 2 is the direct and exclusive final electron acceptor (10).…”

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confidence: 99%

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A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀

et al. 2011

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Sulfite, produced for instance during amino acid metabolism, is a very reactive and toxic compound. Various detoxification mechanisms exist, but sulfite oxidoreductases (SORs) are one of the major actors in sulfite remediation in bacteria and animals. Here we describe the existence of an operon in the extreme thermophilic bacterium Thermus thermophilus HB8 encoding both a SOR and a diheme c-type cytochrome. The in vitro analysis clearly showed that the newly identified cytochrome c 550 acts as an acceptor of the electrons generated by the SOR enzyme during the oxidation of sulfite. The electrons are then rapidly shuttled via cytochrome c 552 to the terminal ba 3 -and caa 3 -type oxidases, thereby unveiling a novel electron transfer pathway, linking sulfite oxidation to oxygen reduction in T. thermophilus: sulfite 3 SOR HB8 3 cytochrome c 550 3 cytochrome c 552 3 ba 3 oxidase/caa 3 oxidase 3 O 2 . The description of the complete pathway reveals that electrons generated during sulfite oxidation by the SOR are funneled into the respiratory chain, participating in the energy production of T. thermophilus.

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Section: Resultsmentioning

confidence: 96%

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A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀

et al. 2011

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“…3 and Table 1). In this regard, it may be significant that the megaplasmid of the hyperthermophilic bacterium Thermus thermophilus possesses a number of genes that are thought to confer growth advantages in thermogenic environments (24). We therefore suggest that this portion of the Sulfolobus genome, including the copG, whiP, stress-related genes, and also the origin itself, was introduced by an extrachromosomal element.…”

Section: Resultsmentioning

confidence: 98%

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes

Robinson

Bell

2007

Proc. Natl. Acad. Sci. U.S.A.

102

108

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In all three domains of life, DNA replication begins at specialized loci termed replication origins. In bacteria, replication initiates from a single, clearly defined site. In contrast, eukaryotic organisms exploit a multitude of replication origins, dividing their genomes into an array of short contiguous units. Recently, the multiple replication origin paradigm has also been demonstrated within the archaeal domain of life, with the discovery that the hyperthermophilic archaeon Sulfolobus has three replication origins. However, the evolutionary mechanism driving the progression from single to multiple origin usage remains unclear. Here, we demonstrate that Aeropyrum pernix, a distant relative of Sulfolobus, has two origins. Comparison with the Sulfolobus origins provides evidence for evolution of replicon complexity by capture of extrachromosomal genetic elements. We additionally identify a previously unrecognized candidate archaeal initiator protein that is distantly related to eukaryotic Cdt1. Our data thus provide evidence that horizontal gene transfer, in addition to its well-established role in contributing to the information content of chromosomes, may fundamentally alter the manner in which the host chromosome is replicated.Archaea ͉ DNA replication ͉ viral integration

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“…This potential is reinforced by progress in the genetic analysis of Thermus thermophilus supported, in turn, by convenient growth and transformation conditions, cloning vectors, selectable markers, and complete genome sequences for two strains (Henne et al 2004;Brü ggemann and Chen 2006). One of these T. thermophilus strains (HB27) lacks a functional reverse gyrase, which is otherwise highly conserved among extremely thermophilic bacteria and archaea (Henne et al 2004).…”

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confidence: 99%

“…One of these T. thermophilus strains (HB27) lacks a functional reverse gyrase, which is otherwise highly conserved among extremely thermophilic bacteria and archaea (Henne et al 2004). In addition, both strains appear to lack family-Y DNA polymerases (Henne et al 2004;Brü ggemann and Chen 2006), which generate many of the mutations induced by DNA damage and may also contribute to spontaneous mutation (Goodman 2002). The apparent lack of these enzymes, combined with the ability to grow .80°, raises questions about the accuracy of genome replication in T. thermophilus.…”

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confidence: 99%

The Rate and Character of Spontaneous Mutation in Thermus thermophilus

et al. 2008

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Selection of spontaneous, loss-of-function mutations at two chromosomal loci (pyrF and pyrE) enabled the first molecular-level analysis of replication fidelity in the extremely thermophilic bacterium Thermus thermophilus. Two different methods yielded similar mutation rates, and mutational spectra determined by sequencing of independent mutants revealed a variety of replication errors distributed throughout the target genes. The genomic mutation rate estimated from these targets, 0.00097 6 0.00052 per replication, was lower than corresponding estimates from mesophilic microorganisms, primarily because of a low rate of base substitution. However, both the rate and spectrum of spontaneous mutations in T. thermophilus resembled those of the thermoacidophilic archaeon Sulfolobus acidocaldarius, despite important molecular differences between these two thermophiles and their genomes.

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Comparative genomics of Thermus thermophilus: Plasticity of the megaplasmid and its contribution to a thermophilic lifestyle

Cited by 65 publications

References 32 publications

A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀

A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes

The Rate and Character of Spontaneous Mutation in Thermus thermophilus

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Comparative genomics of Thermus thermophilus: Plasticity of the megaplasmid and its contribution to a thermophilic lifestyle

Cited by 65 publications

References 32 publications

A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c 550

A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c 550

Extrachromosomal element capture and the evolution of multiple replication origins in archaeal chromosomes

The Rate and Character of Spontaneous Mutation in Thermus thermophilus

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A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀

A Sulfite Respiration Pathway from Thermus thermophilus and the Key Role of Newly Identified Cytochrome c ₅₅₀