Comparative Genomics of Botrytis cinerea Strains with Differential Multi-Drug Resistance

Chatzidimopoulos, Michael; Psomopoulos, Fotis; Malandrakis, Emmanouil E.; Ganopoulos, Ioannis; Madesis, Panagiotis; Vellios, Evangelos; Drogoudi, P.

doi:10.3389/fpls.2016.00554

Cited by 4 publications

(3 citation statements)

References 14 publications

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“…Over 1000 plant species worldwide are affected by B. cinerea ; the second most prevalent pathogenic plant fungus (Reboledo et al, 2020 ). Antifungal resistance, including MDR, has become a growing problem with B. cinerea infections (Chatzidimopoulos et al, 2016 ), necessitating the development of novel antifungal agents to manage these disease outbreaks which affect a large variety of crops, for example tomato, lettuce, beans, strawberry, grape, etc.…”

Section: Antimicrobial Activity Of Betalainsmentioning

confidence: 99%

Antimicrobial betalains

Wijesinghe

Choo

2022

Journal of Applied Microbiology

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Betalains are nitrogen-containing plant pigments that can be red-violet (betacyanins) or yellow-orange (betaxanthins), currently employed as natural colourants in the food and cosmetic sectors. Betalains exhibit antimicrobial activity against a broad spectrum of microbes including multidrug-resistant bacteria, as well as single-species and dual-species biofilm-producing bacteria, which is highly significant given the current antimicrobial resistance issue reported by The World Health Organization.Research demonstrating antiviral activity against dengue virus, in silico studies including SARS-CoV-2, and anti-fungal effects of betalains highlight the diversity of their antimicrobial properties. Though limited in vivo studies have been conducted, antimalarial and anti-infective activities of betacyanin have been observed in living infection models. Cellular mechanisms of antimicrobial activity of betalains are yet unknown; however existing research has laid the framework for a potentially novel antimicrobial agent. This review covers an overview of betalains as antimicrobial agents and discussions to fully exploit their potential as therapeutic agents to treat infectious diseases.

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Section: Antimicrobial Activity Of Betalainsmentioning

confidence: 99%

Antimicrobial betalains

Wijesinghe

Choo

2022

Journal of Applied Microbiology

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“…However, under long-term selective pressure, B. cinerea has developed varying degrees of tolerance to almost all fungicides, which severely weakens the control efficacy of these fungicides on grey mould (Hu et al 2016). Point mutations in drug targets of B. cinerea are mainly responsible for high levels of resistance to fungicides with site-specific activity (Chatzidimopoulos et al 2016). Therefore, new targets need to be identified to explore more effective fungicides to control the occurrence of B. cinerea.…”

Section: Introductionmentioning

confidence: 99%

An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

et al. 2022

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Peroxisomes are very important organelles in eukaryotic cells and participate in various biological processes, including pathogen–host interactions. A variety of proteins involved in peroxisome proliferation and metabolic activity within peroxisomes have been shown to be essential for full virulence of several phytopathogenic fungi. However, the effects of changes in the number of peroxisomes and proteins involved in the peroxisome pathway on the pathogenicity of Botrytis cinerea have rarely been reported. In this study, by analysing transcriptome data and RT-qPCR validation, we found that more than half of the genes annotated to the peroxisome pathway in B. cinerea were upregulated more than twofold between mycelial samples cultured in medium with tomato leaves and without tomato leaves. A strain of B. cinerea with fluorescently labelled peroxisomes was obtained by overexpression of GFP fused to peroxisomal targeting signal 1 (the tripeptide ‘SKL’). The addition of tomato leaves to the liquid medium induced a significant increase in the number of peroxisomes, β-oxidation level, H2O2 content, and acetyl-CoA level in B. cinerea mycelia. When B. cinerea was cultured with oleic acid as the sole carbon source, the formation of infection-related structures and their penetration into plant cells were found to be associated with peroxisome pathway activity. Furthermore, peroxisome proliferation and lipid metabolism increased in response to different extracellular stresses in B. cinerea. Taken together, our results confirmed that activation of the peroxisome pathway in B. cinerea contributes to the initial infection and the ability to cope with environmental stress.

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“…The anilinopyrimidine fungicides such as pyrimethanil, cyprodinil and mepanipyrim have been launched for the control of various ascomycetes pathogens, especially the gray mold on fruits, vegetables and ornamentals [ 4 , 5 ]. Anilinopyrimidines are synthetic drugs and although effective, resistant isolates have been frequently reported due to the increasing amounts of applied fungicides [ 6 , 7 ]. Moreover, B. cinerea is one of the high frequencies of drug-resistant pathogens due to its high genetic variability, short life cycle, and prolific reproduction [ 8 ].…”

Section: Introductionmentioning

confidence: 99%

Synergistic Effects of Resveratrol and Pyrimethanil against Botrytis cinerea on Grape

et al. 2018

Molecules

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Botrytis cinerea is the pathogen of gray mold disease affecting a wide range of plant hosts, with consequential economic losses worldwide. The increased frequency of fungicide resistance of the pathogen challenges its disease management, and thus the development of alternative control strategies are urgently required. In this study, we showed excellent synergistic interactions between resveratrol and pyrimethanil. Significant synergistic values were recorded by the two-drug combination on the suppression of mycelial growth and conidia germination of B. cinerea. The combination of resveratrol and pyrimethanil caused malformation of mycelia. Moreover, the inoculation assay was conducted on table grape and consistent synergistic suppression of the two-drug combination was found in vivo. Our findings first revealed that the combination of resveratrol and pyrimethanil has synergistic effects against resistant B. cinerea and support the potential use of resveratrol as a promising adjuvant on the control of gray mold.

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Comparative Genomics of Botrytis cinerea Strains with Differential Multi-Drug Resistance

Cited by 4 publications

References 14 publications

Antimicrobial betalains

Antimicrobial betalains

An increase in the number of peroxisomes is coupled to the initial infection stage and stress response of Botrytis cinerea

Synergistic Effects of Resveratrol and Pyrimethanil against Botrytis cinerea on Grape

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