Comparative Genomics of Accessory Gland Protein Genes in Drosophila melanogaster and D. pseudoobscura

Wagstaff, Bradley J.; Begun, David J.

doi:10.1093/molbev/msi067

Cited by 60 publications

(63 citation statements)

References 55 publications

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“…We therefore believe that different rates of gain or loss between species account for the majority of our observations, such that different species of Drosophila use different complements of SFPs and, to a lesser extent, testes-expressed genes. These data are consistent with previous studies finding novel SFPs in other Drosophila species (Begun and Lindfors 2005;Wagstaff and Begun 2005a;Begun et al 2006).…”

Section: Female Reproductive Tractsupporting

confidence: 93%

“…Such studies reported that genes expressed in the testis and genes encoding seminal fluid proteins include many that evolve rapidly (Begun et al 2000Swanson et al 2001;Holloway and Begun 2004;Kern et al 2004;Zhang et al 2004;Begun and Lindfors 2005;Mueller et al 2005;Wagstaff and Begun 2005a;Schully and Hellberg 2006). Many female reproductive proteins have also been shown to evolve faster than non-sexspecific proteins (Civetta and Singh 1995;Jansa et al 2003;Swanson et al 2004;Jagadeeshan and Singh 2005).…”

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confidence: 99%

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Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

et al. 2007

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A large portion of the annotated genes in Drosophila melanogaster show sex-biased expression, indicating that sex and reproduction-related genes (SRR genes) represent an appreciable component of the genome. Previous studies, in which subsets of genes were compared among few Drosophila species, have found that SRR genes exhibit unusual evolutionary patterns. Here, we have used the newly released genome sequences from 12 Drosophila species, coupled to a larger set of SRR genes, to comprehensively test the generality of these patterns. Among 2505 SRR genes examined, including ESTs with biased expression in reproductive tissues and genes characterized as involved in gametogenesis, we find that a relatively high proportion of SRR genes have experienced accelerated divergence throughout the genus Drosophila. Several testis-specific genes, male seminal fluid proteins (SFPs), and spermatogenesis genes show lineage-specific bursts of accelerated evolution and positive selection. SFP genes also show evidence of lineage-specific gene loss and/ or gain. These results bring us closer to understanding the details of the evolutionary dynamics of SRR genes with respect to species divergence.

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Section: Female Reproductive Tractsupporting

confidence: 93%

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confidence: 99%

Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

et al. 2007

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“…This is below the similarity level detectable in our search for D. pseudoobscura orthologs. For another gene, Acp95EF, our analysis revealed its D. pseudoobscura ortholog, which was undetected by Wagstaff and Begun (2005). Differences in methodologies and the limited alignability of the D. pseudoobscura genome (only 48%; Richards et al 2005) likely account for these two differences in Acp ortholog detection.…”

Section: Methodsmentioning

confidence: 83%

“…D. melanogaster ACPs undetectable in D. pseudoobscura via our methods could be highly diverged, located in an unsequenced region of the D. pseudoobscura genome, or potential D. melanogaster lineage-specific proteins. A recent study (Wagstaff and Begun 2005) uncovered a D. pseudoobscura gene with 18.5% amino acid sequence identity to D. melanogaster Acp26Aa. This is below the similarity level detectable in our search for D. pseudoobscura orthologs.…”

Section: Methodsmentioning

confidence: 99%

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

et al. 2005

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Drosophila melanogaster males transfer seminal fluid proteins along with sperm during mating. Among these proteins, ACPs (Accessory gland proteins) from the male's accessory gland induce behavioral, physiological, and life span reduction in mated females and mediate sperm storage and utilization. A previous evolutionary EST screen in D. simulans identified partial cDNAs for 57 new candidate ACPs. Here we report the annotation and confirmation of the corresponding Acp genes in D. melanogaster. Of 57 new candidate Acp genes previously reported in D. melanogaster, 34 conform to our more stringent criteria for encoding putative male accessory gland extracellular proteins, thus bringing the total number of ACPs identified to 52 (34 plus 18 previously identified). This comprehensive set of Acp genes allows us to dissect the patterns of evolutionary change in a suite of proteins from a single male-specific reproductive tissue. We used sequence-based analysis to examine codon bias, gene duplications, and levels of divergence

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“…Previous work has demonstrated that Acp's evolve more rapidly than most Drosophila genes (Begun et al 2000;Swanson et al 2001;Wagstaff and Begun 2005a) and that they evolve especially rapidly in desert Drosophila of the repleta group (Wagstaff and Begun 2005b). Elevated rates of Acp evolution in desert Drosophila may be due to differences between their mating system and that of flies from the D. melanogaster subgroup (Markow 1996(Markow , 2002.…”

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confidence: 99%

Adaptive Evolution of Recently Duplicated Accessory Gland Protein Genes in Desert Drosophila

Wagstaff

Begun

2007

Genetics

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The relationship between animal mating system variation and patterns of protein polymorphism and divergence is poorly understood. Drosophila provides an excellent system for addressing this issue, as there is abundant interspecific mating system variation. For example, compared to D. melanogaster subgroup species, repleta group species have higher remating rates, delayed sexual maturity, and several other interesting differences. We previously showed that accessory gland protein genes (Acp's) of Drosophila mojavensis and D. arizonae evolve more rapidly than Acp's in the D. melanogaster subgroup and that adaptive Acp protein evolution is likely more common in D. mojavensis/D. arizonae than in D. melanogaster/D. simulans. These findings are consistent with the idea that greater postcopulatory selection results in more adaptive evolution of seminal fluid proteins in the repleta group flies. Here we report another interesting evolutionary difference between the repleta group and the D. melanogaster subgroup Acp's. Acp gene duplications are present in D. melanogaster, but their high sequence divergence indicates that the fixation rate of duplicated Acp's has been low in this lineage. Here we report that D. mojavensis and D. arizonae genomes contain several very young duplicated Acp's and that these Acp's have experienced very rapid, adaptive protein divergence. We propose that rapid remating of female desert Drosophila generates selection for continuous diversification of the male Acp complement to improve male fertilization potential. Thus, mating system variation may be associated with adaptive protein divergence as well as with duplication of Acp's in Drosophila.

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Comparative Genomics of Accessory Gland Protein Genes in Drosophila melanogaster and D. pseudoobscura

Cited by 60 publications

References 55 publications

Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

Evolution in the Fast Lane: Rapidly Evolving Sex-Related Genes in Drosophila

Cross-Species Comparison of Drosophila Male Accessory Gland Protein Genes

Adaptive Evolution of Recently Duplicated Accessory Gland Protein Genes in Desert Drosophila

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