Comparative genomics identifies the <scp>M</scp>agnaporthe oryzae avirulence effector <scp>A</scp>vr<scp>P</scp>i9 that triggers <scp>P</scp>i9‐mediated blast resistance in rice

Wu, Jun; Kou, Yanjun; Bao, Jiandong; Wang, Yongdong; Tang, Mingzhi; Zhu, Xiaoli; Ponaya, Ariane; Xiao, Gui; Li, Jinbin; Li, Chenyun; Song, Min-Young; Cumagun, Christian Joseph R.; Deng, Qipan; Lu, Guodong; Jeon, Jong‐Seong; Naqvi, Naweed I.; Zhou, Bo

doi:10.1111/nph.13310

Cited by 156 publications

(95 citation statements)

References 56 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The resulting drastic divergences in micro- and macro-genomic regions that should be the major impediment, which can sometimes stop chromosome walking to the target locus in the map-based cloning process19303132. The complementary and/or alternative approaches applied in such cases were association genetics33, comparative genomics20, and comparative transcriptomics32, all of which rely on the high throughput capacity of current DNA sequencing technologies. In the current study, the AvrPib has been successfully isolated by map-based cloning, in silico , in which even the chromosome and its micro-genomic region containing the target locus was rearranged based on the recombinants detected among the polymorphic markers those were developed based on the reference sequence of isolate 70-15 (Fig.…”

Section: Discussionmentioning

confidence: 99%

“…The rice blast pathosystem has emerged as an effective model for studying gene-for-gene interactions314. Of the >100 recognized major host R genes, 22 have been successfully isolated to date715161718, while the same is true for eight of the >40 Avr genes known5161920. In contrast to the host gene products, which are almost members of the NBS-LRR family of proteins, there is little evidence of any commonality among the Avr products4151621.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib

Zhang

Wang

et al. 2015

Sci Rep

113

129

View full text Add to dashboard Cite

Magnaporthe oryzae (Mo) is the causative pathogen of the damaging disease rice blast. The effector gene AvrPib, which confers avirulence to host carrying resistance gene Pib, was isolated via map-based cloning. The gene encodes a 75-residue protein, which includes a signal peptide. Phenotyping and genotyping of 60 isolates from each of five geographically distinct Mo populations revealed that the frequency of virulent isolates, as well as the sequence diversity within the AvrPib gene increased from a low level in the far northeastern region of China to a much higher one in the southern region, indicating a process of host-driven selection. Resequencing of the AvrPiballele harbored by a set of 108 diverse isolates revealed that there were four pathoways, transposable element (TE) insertion (frequency 81.7%), segmental deletion (11.1%), complete absence (6.7%), and point mutation (0.6%), leading to loss of the avirulence function. The lack of any TE insertion in a sample of non-rice infecting Moisolates suggested that it occurred after the host specialization of Mo. Both the deletions and the functional point mutation were confined to the signal peptide. The reconstruction of 16 alleles confirmed seven functional nucleotide polymorphisms for the AvrPiballeles, which generated three distinct expression profiles.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib

Zhang

Wang

et al. 2015

Sci Rep

113

129

View full text Add to dashboard Cite

show abstract

“…To date, 11 avirulence ( Avr ) genes in rice blast fungus have been cloned. Nine out of 11 genes, namely; AvrPita (Orbach et al 2000), ACE1 (Bohnert et al 2004), AvrPia , AvrPii , AvrPik (Yoshida et al 2009), AvrPiz-t (Li et al 2009), Avr1-CO3 9 (Zheng et al 2011), AvrPib (Zhang et al 2015), and AvrPi9 (Wu et al 2015), have their corresponding R genes in rice. However, there are no known R genes in rice for PWL1 (Kang et al 1995) and PWL2 (Sweigard et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Chaipanya

Telebanco‐Yanoria

Quime

et al. 2017

Rice

Self Cite

View full text Add to dashboard Cite

BackgroundRice (Oryza sativa) is one of the most important food crops in the world. Rice blast, caused by the fungal pathogen Magnaporthe oryzae, is one of the most destructive rice diseases worldwide. To effectively cope with this problem, the use of rice blast resistance varieties through innovative breeding programs is the best strategy to date. The Thai rice variety Jao Hom Nin (JHN) showed broad-spectrum resistance against Thai rice blast isolates. Two QTLs for blast resistance in JHN were reported on chromosome 1 (QTL1) and 11 (QTL11).ResultsMonogenic lines of QTL1 (QTL1-C) and QTL11 (QTL11-C) in the CO39 genetic background were generated. Cluster analysis based on the disease reaction pattern of QTL1-C and QTL11-C, together with IRBLs, showed that those two monogenic lines were clustered with IRBLsh-S (Pish) and IRBL7-M (Pi7), respectively. Moreover, sequence analysis revealed that Pish and Pi7 were embedded within the QTL1 and QTL11 delimited genomic intervals, respectively. This study thus concluded that QTL1 and QTL11 could encode alleles of Pish and Pi7, designated as Pish-J and Pi7-J, respectively. To validate this hypothesis, the genomic regions of Pish-J and Pi7-J were cloned and sequenced. Protein sequence comparison revealed that Pish-J and Pi7-J were identical to Pish and Pi7, respectively. The holistic disease spectrum of JHN was found to be exactly attributed to the additive ones of both QTL1-C and QTL11-C.ConclusionJHN showed broad spectrum resistance against Thai and Philippine rice blast isolates. As this study demonstrated, the combination of two resistance genes, Pish-J and Pi7-J, in JHN, with each controlling broad-spectrum resistance to rice blast disease, explains the high level of resistance. Thus, the combination of Pish and Pi7 can provide a practical scheme for breeding durable resistance in rice against rice blast disease.Electronic supplementary materialThe online version of this article (doi:10.1186/s12284-017-0159-0) contains supplementary material, which is available to authorized users.

show abstract

“…In this study, we describe the cloning and functional characterization of Pi50, the 4th R gene at the Pi2/9 locus, in addition to Pi2, Pi9, and Piz-t Zhou et al 2006). As described in Table 1, both GUY11 (without AvrPiz-t and AvrPi2, Li et al 2009) and R01-1 (without AvrPi9, Wu et al 2015) are avirulent to Pi50 ( -47, 12-3058, 13-856) showing reciprocally avirulent and virulent reactions to Pi50, Pi2, Pi9, and Piz-t were used for inoculation. Interestingly, their resistance spectra can be clearly differentiated with different sets of M. oryzae isolates, suggesting that these four R genes recognize different Avr effectors (Liu et al 2002;Li et al 2009; this study).…”

Section: Discussionmentioning

confidence: 99%

“…The proteins encoded by these four genes share greater than 96 % amino acid sequence identity to one another and their sequence differences disproportionally occur within the LRR domain ; this study). Dashes identical nucleotides to the consensus sequence either AvrPiz-t or AvrPi9 (Li et al 2009;Wu et al 2015). As described in Table 1, both GUY11 (without AvrPiz-t and AvrPi2, Li et al 2009) and R01-1 (without AvrPi9, Wu et al 2015) are avirulent to Pi50 ( -47, 12-3058, 13-856) showing reciprocally avirulent and virulent reactions to Pi50, Pi2, Pi9, and Piz-t were used for inoculation.…”

Section: Discussionmentioning

confidence: 99%

Functional divergence of duplicated genes results in a novel blast resistance gene Pi50 at the Pi2/9 locus

Wang

Han

et al. 2015

Theor Appl Genet

Self Cite

View full text Add to dashboard Cite

We characterized a novel blast resistance gene Pi50 at the Pi2/9 locus; Pi50 is derived from functional divergence of duplicated genes. The unique features of Pi50 should facilitate its use in rice breeding and improve our understanding of the evolution of resistance specificities. Rice blast disease, caused by the fungal pathogen Magnaporthe oryzae, poses constant, major threats to stable rice production worldwide. The deployment of broad-spectrum resistance (R) genes provides the most effective and economical means for disease control. In this study, we characterize the broad-spectrum R gene Pi50 at the Pi2/9 locus, which is embedded within a tandem cluster of 12 genes encoding proteins with nucleotide-binding site and leucine-rich repeat (NBS-LRR) domains. In contrast with other Pi2/9 locus, the Pi50 cluster contains four duplicated genes (Pi50_NBS4_1 to 4) with extremely high nucleotide sequence similarity. Moreover, these duplicated genes encode two kinds of proteins (Pi50_NBS4_1/2 and Pi50_NBS4_3/4) that differ by four amino acids. Complementation tests and resistance spectrum analyses revealed that Pi50_NBS4_1/2, not Pi50_NBS4_3/4, control the novel resistance specificity as observed in the Pi50 near isogenic line, NIL-e1. Pi50 shares greater than 96 % amino acid sequence identity with each of three other R proteins, i.e., Pi9, Piz-t, and Pi2, and has amino acid changes predominantly within the LRR region. The identification of Pi50 with its novel resistance specificity will facilitate the dissection of mechanisms behind the divergence and evolution of different resistance specificities at the Pi2/9 locus.

show abstract

Comparative genomics identifies the Magnaporthe oryzae avirulence effector AvrPi9 that triggers Pi9‐mediated blast resistance in rice

Cited by 156 publications

References 56 publications

Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib

Function and evolution of Magnaporthe oryzae avirulence gene AvrPib responding to the rice blast resistance gene Pib

Dissection of broad-spectrum resistance of the Thai rice variety Jao Hom Nin conferred by two resistance genes against rice blast

Functional divergence of duplicated genes results in a novel blast resistance gene Pi50 at the Pi2/9 locus

Contact Info

Product

Resources

About