Comparative Genomics and Physiological Investigation of a New Arthrospira/Limnospira Strain O9.13F Isolated from an Alkaline, Winter Freezing, Siberian Lake

Misztak, Agnieszka; Waleron, Małgorzata; Furmaniak, Magda; Bazhenova, O. P.; Daroch, Maurycy; Waleron, Krzysztof

doi:10.3390/cells10123411

Cited by 11 publications

(6 citation statements)

References 96 publications

(141 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1999), Arthrospira Stizenberger ex Gomont (Miklaszewska et al . 2012; Misztak et al . 2021), Synechococcus , Planktothrix , Pseudanabaena Lauterborn, Nodularia, Nostoc , Calothrix (Miklaszewska et al .…”

Section: Resultsunclassified

Structural and Functional Genes, and Highly Repetitive Sequences Commonly Used in the Phylogeny and Species Concept of the Phylum Cyanobacteria

Nowruzi

Hutárová

2023

Cryptogamie, Algologie

View full text Add to dashboard Cite

Cyanobacteria are a lineage of Eubacteria that have long captured the attention of scientists. Approximately 5310 species of cyanobacteria have been hitherto described and new species are continually being found, named and described according to established rules. The correct determination of cyanobacteria strains concerns new biotechnological applications as well as ecological studies. There are many situations where it is crucial to recognise distinct algae species, however methods for doing so vary greatly. The aim of this review is to summarize the state of the art of the main and most recent molecular studies focusing on the phylum Cyanobacteria, with particular attention to the most frequently used gene markers. For a long time, the classification method used for cyanobacteria as well as traditionally described species was mainly based on morphology. Over time, integrative taxonomy, which involves the inclusion of many characters and comprehensive taxa sampling, has become the rule as it provides a better resolution of species relationships. For a better resolution of the phylogenies of the phylum Cyanobacteria, it is usually necessary to focus on different genetic markers: from the most common, like the 16S and 23S rRNA, ITS, rbcLXS and rpoC genes, to genes not so widely used, such as hetR, psbA, tufA, gyp and cpcBA. Also, the highly repetitive sequences often used for the symbiotic cyanobacteria represent an important factor in the inference of the phylogenetic relationships.

show abstract

Section: Resultsunclassified

Structural and Functional Genes, and Highly Repetitive Sequences Commonly Used in the Phylogeny and Species Concept of the Phylum Cyanobacteria

Nowruzi

Hutárová

2023

Cryptogamie, Algologie

View full text Add to dashboard Cite

show abstract

“…and it is, therefore, premature to conclude how these two species differ. Nonetheless, a recent comparative genomic analysis (Misztak et al, 2021) proposed that the two species are, in fact, in two different Arthrospira groups, thus questioning whether tool transferability could be achieved in these two hosts.…”

Section: Limited Genetic Tools Have Been Developed and Applied In Mar...mentioning

confidence: 99%

Synthetic biology in marine cyanobacteria: Advances and challenges

Bourgade

Stensjö

2022

Front. Microbiol.

View full text Add to dashboard Cite

The current economic and environmental context requests an accelerating development of sustainable alternatives for the production of various target compounds. Biological processes offer viable solutions and have gained renewed interest in the recent years. For example, photosynthetic chassis organisms are particularly promising for bioprocesses, as they do not require biomass-derived carbon sources and contribute to atmospheric CO2 fixation, therefore supporting climate change mitigation. Marine cyanobacteria are of particular interest for biotechnology applications, thanks to their rich diversity, their robustness to environmental changes, and their metabolic capabilities with potential for therapeutics and chemicals production without requiring freshwater. The additional cyanobacterial properties, such as efficient photosynthesis, are also highly beneficial for biotechnological processes. Due to their capabilities, research efforts have developed several genetic tools for direct metabolic engineering applications. While progress toward a robust genetic toolkit is continuously achieved, further work is still needed to routinely modify these species and unlock their full potential for industrial applications. In contrast to the understudied marine cyanobacteria, genetic engineering and synthetic biology in freshwater cyanobacteria are currently more advanced with a variety of tools already optimized. This mini-review will explore the opportunities provided by marine cyanobacteria for a greener future. A short discussion will cover the advances and challenges regarding genetic engineering and synthetic biology in marine cyanobacteria, followed by a parallel with freshwater cyanobacteria and their current genetic availability to guide the prospect for marine species.

show abstract

“…Cyanobacteria from the genus Arthrospira/Limnospira are considered haloalkalo-tolerant organisms with optimal growth temperatures of approximately 35 °C. They are most abundant in soda lakes in tropical and subtropical regions [ 1 ]. Indeed, the paper by Misztak et al is devoted to the comprehensive genome-based characterization and physiological investigation of the new strain O9.13F, which was isolated from the winter freezing Solenoye Lake in Western Siberia [ 1 ].…”

mentioning

confidence: 99%

“…They are most abundant in soda lakes in tropical and subtropical regions [ 1 ]. Indeed, the paper by Misztak et al is devoted to the comprehensive genome-based characterization and physiological investigation of the new strain O9.13F, which was isolated from the winter freezing Solenoye Lake in Western Siberia [ 1 ]. Comparative genomics showed that no unique genes were found for the Siberian strain related to its tolerance to low temperatures and high salinity, while the fatty acid composition was specific and unique to this strain.…”

mentioning

confidence: 99%

“…Comparative genomics showed that no unique genes were found for the Siberian strain related to its tolerance to low temperatures and high salinity, while the fatty acid composition was specific and unique to this strain. The authors showed that the optimal cultivation temperature for strain O9.13F (20 °C) is lower than that for Arthrospira/Limnospira strains (35 °C) [ 1 ].…”

mentioning

confidence: 99%

See 1 more Smart Citation