Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii

Kabić, Jovana; Novović, Katarina; Kekić, Dušan; Trudić, Anika; Opavski, Nataša; Dimkić, Ivica; Jovčić, Branko; Gajić, Ina

doi:10.1016/j.csbj.2022.12.045

Cited by 13 publications

(12 citation statements)

References 51 publications

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“…Accordingly, pmrB mutations could lead to the constitutive activation of PmrA, resulting in increased expression of the pmrCAB and resistance to colistin [ 59 ]. In addition, previous studies reported frequent amino acid substitutions of PmrB at position 170 (P to Y, L, Q, or S) ( Table 1 ) and 315 (G to D, S, or V) in colistin-resistant isolates [ 68 , 70 , 76 , 84 , 86 ]. Although Oikonomou and coauthors [ 69 ] described the PmrB mutations (A138T, A226V, and A444V) repeated in colistin-resistant A. baumannii [ 70 , 72 , 73 , 74 , 76 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] as not responsible for colistin resistance, the involvement of A138T and A226V in this phenomenon should not be excluded.…”

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 92%

“…In addition, previous studies reported frequent amino acid substitutions of PmrB at position 170 (P to Y, L, Q, or S) ( Table 1 ) and 315 (G to D, S, or V) in colistin-resistant isolates [ 68 , 70 , 76 , 84 , 86 ]. Although Oikonomou and coauthors [ 69 ] described the PmrB mutations (A138T, A226V, and A444V) repeated in colistin-resistant A. baumannii [ 70 , 72 , 73 , 74 , 76 , 84 , 85 , 86 , 87 , 88 , 89 , 90 , 91 ] as not responsible for colistin resistance, the involvement of A138T and A226V in this phenomenon should not be excluded. Indeed, the amino acid change at position 226 (A to V) in PmrB alone or in combination with A138T enabled high colistin resistance (64 or 128 and 256, respectively) in the tested isolates [ 84 , 88 ].…”

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 92%

“…Although the amino acid substitutions N287D ( lpxC ) and E117K ( lpxD ) were detected in both colistin-susceptible and colistin-resistant isolates, it is possible that these alterations in combination with a mutation in the pmrCAB operon have a synergistic effect leading to colistin resistance [ 69 , 70 , 71 , 72 , 73 , 74 ]. In addition, the downregulation of lpxACD expression has been observed in some colistin-resistant A. baumannii isolates, leading to decreased LPS production [ 68 , 73 , 74 , 75 , 76 ].…”

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 99%

“…To date, little data are available on the relationship between PmrC amino acid changes and colistin resistance. A comparison of PmrC amino acid sequences between colistin-susceptible and colistin-resistant isolates revealed rare changes and mostly at different positions [ 65 , 69 , 72 , 73 , 74 , 75 , 76 , 84 , 89 , 95 , 97 ]. In the study conducted by Nurtop and coauthors [ 72 ], the two most commonly described mutations in the pmrC gene (resulting in I42V and L150F) were found to be associated with an increased expression of the pmrA and pmrC genes and, consequently, colistin resistance.…”

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 99%

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Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology

Novović

Jovčić

2023

Antibiotics

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Acinetobacter baumannii is recognized as a clinically significant pathogen causing a wide spectrum of nosocomial infections. Colistin was considered a last-resort antibiotic for the treatment of infections caused by multidrug-resistant A. baumannii. Since the reintroduction of colistin, a number of mechanisms of colistin resistance in A. baumannii have been reported, including complete loss of LPS by inactivation of the biosynthetic pathway, modifications of target LPS driven by the addition of phosphoethanolamine (PEtN) moieties to lipid A mediated by the chromosomal pmrCAB operon and eptA gene-encoded enzymes or plasmid-encoded mcr genes and efflux of colistin from the cell. In addition to resistance to colistin, widespread heteroresistance is another feature of A. baumannii that leads to colistin treatment failure. This review aims to present a critical assessment of relevant published (>50 experimental papers) up-to-date knowledge on the molecular mechanisms of colistin resistance in A. baumannii with a detailed review of implicated mutations and the global distribution of colistin-resistant strains.

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Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 92%

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 92%

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 99%

Section: Molecular Mechanisms Of Colistin Resistance In a B...mentioning

confidence: 99%

See 2 more Smart Citations

Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology

Novović

Jovčić

2023

Antibiotics

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“…The second report described the WGS (Illumina MiSeq) of 30 colistin-resistant A. baumannii isolates and analyzed the global genomic epidemiology of these infectious agents [ 210 ]. Phylogenomic analysis showed that colistin resistance arose independently in several clonal lineages.…”

Section: A Baumanniimentioning

confidence: 99%

Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Peykov

2023

Microorganisms

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Non-fermenting Gram-negative bacilli (NFGNB), such as Pseudomonas aeruginosa and Acinetobacter baumannii, are among the major opportunistic pathogens involved in the global antibiotic resistance epidemic. They are designated as urgent/serious threats by the Centers for Disease Control and Prevention and are part of the World Health Organization’s list of critical priority pathogens. Also, Stenotrophomonas maltophilia is increasingly recognized as an emerging cause for healthcare-associated infections in intensive care units, life-threatening diseases in immunocompromised patients, and severe pulmonary infections in cystic fibrosis and COVID-19 individuals. The last annual report of the ECDC showed drastic differences in the proportions of NFGNB with resistance towards key antibiotics in different European Union/European Economic Area countries. The data for the Balkans are of particular concern, indicating more than 80% and 30% of invasive Acinetobacter spp. and P. aeruginosa isolates, respectively, to be carbapenem-resistant. Moreover, multidrug-resistant and extensively drug-resistant S. maltophilia from the region have been recently reported. The current situation in the Balkans includes a migrant crisis and reshaping of the Schengen Area border. This results in collision of diverse human populations subjected to different protocols for antimicrobial stewardship and infection control. The present review article summarizes the findings of whole-genome sequencing-based resistome analyses of nosocomial multidrug-resistant NFGNBs in the Balkan countries.

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Antibiotics: From Mechanism of Action to Resistance and Beyond

Saikia,

Chetia

2024

Indian J Microbiol

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Comparative genomics and molecular epidemiology of colistin-resistant Acinetobacter baumannii

Cited by 13 publications

References 51 publications

Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology

Colistin Resistance in Acinetobacter baumannii: Molecular Mechanisms and Epidemiology

Whole-Genome Sequencing-Based Resistome Analysis of Nosocomial Multidrug-Resistant Non-Fermenting Gram-Negative Pathogens from the Balkans

Antibiotics: From Mechanism of Action to Resistance and Beyond

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