Comparative genomics analysis of two banana Fusarium wilt biocontrol endophytes Bacillus subtilis R31 and TR21 provides insights into their differences on phytobeneficial trait

Li, Chunji; Cheng, Ping; Zheng, Li; Li, Yongjian; Chen, Yanhong; Wen, Shuheng; Gui, Yu

doi:10.1016/j.ygeno.2021.02.006

Cited by 20 publications

(22 citation statements)

References 83 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Dai ( Yajing, 2018 ) detected the quantity of different PGPR in banana soil rhizosphere by fluorescence quantitative PCR. The results indicated that the biomass of three antagonistic bacteria in banana rhizosphere soil was increased significantly, indicating that the three PGPR strains M8, C5, C14 can colonize banana roots ( Lin, 2011 ). Their colonization determination studies used antibiotic-labeled strains and showed that labeled strains could be isolated by wound inoculation, irrigated inoculation, and axil inoculation.…”

Section: Discussionmentioning

confidence: 99%

Monitoring Tritrophic Biocontrol Interactions Between Bacillus spp., Fusarium oxysporum f. sp. cubense, Tropical Race 4, and Banana Plants in vivo Based on Fluorescent Transformation System

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

Bacillus spp. is effective biocontrol agents for Fusarium wilt of banana (FWB), tropical race 4 (TR4). This study explores the colonization by Bacillus subtilis, Bacillus velezensis, and Bacillus amyloliquefaciens of host banana plants and elucidates the mechanism of antagonistic TR4 biocontrol. The authors selected one B. subtilis strain, three B. velezensis strains, and three B. amyloliquefaciens strains that are proven to significantly inhibit TR4 in vitro, optimized the genetic transformation conditions and explored their colonization process in banana plants. The results showed that we successfully constructed an optimized fluorescent electro-transformation system (OD600 of bacteria concentration=0.7, plasmid concentration=50ng/μl, plasmid volume=2μl, transformation voltage=1.8kV, and transformation capacitance=400Ω) of TR4-inhibitory Bacillus spp. strains. The red fluorescent protein (RFP)-labeled strains were shown to have high stability with a plasmid-retention frequency above 98%, where bacterial growth rates and TR4 inhibition are unaffected by fluorescent plasmid insertion. In vivo colonizing observation by Laser Scanning Confocal Microscopy (LSCM) and Scanning Electron Microscopy (SEM) showed that Bacillus spp. can colonize the internal cells of banana plantlets roots. Further, fluorescent observation by LSCM showed these RFP-labeled bacteria exhibit chemotaxis (chemotaxis ratio was 1.85±0.04) toward green fluorescent protein (GFP)-labeled TR4 hyphae in banana plants. We conclude that B. subtilis, B. velezensis, and B. amyloliquefaciens can successfully colonize banana plants and interact with TR4. Monitoring its dynamic interaction with TR4 and its biocontrol mechanism is under further study.

show abstract

Section: Discussionmentioning

confidence: 99%

Monitoring Tritrophic Biocontrol Interactions Between Bacillus spp., Fusarium oxysporum f. sp. cubense, Tropical Race 4, and Banana Plants in vivo Based on Fluorescent Transformation System

et al. 2021

Front. Microbiol.

View full text Add to dashboard Cite

show abstract

“…The isolation and identification of B. subtilis R31 have been described in detail in a previous study ( Li et al, 2021a ). Here, we briefly describe the preparation process of the bacterial suspension.…”

Section: Methodsmentioning

confidence: 99%

“…At present, there are mainly two types of microbial biological control measures for plant diseases: one is to introduce exogenous antagonistic bacteria into crops or soil, and the other is to regulate the environmental microbial community of plant growth so that the existing beneficial microbial community can increase and show antagonistic activity ( Meng et al, 2018 ; Futo et al, 2021 ). Some biocontrol bacteria can produce antibacterial substances and effectively inhibit the growth of pathogenic bacteria, which is called the antibacterial effect ( Seedorf et al, 2008 ; Li et al, 2021a ). Studies have shown a close relationship between plants and microorganisms in the soil ( Wu et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

“…The endophytic B. subtilis R31 isolated from the leaves of Dendrobium orchid is a typical example of an exogenous endophyte for controlling banana fusarium wilt in China ( Li et al, 2021a ). The strain and its patent have been industrialized by Guangdong Geolong Biotechnology Co., Ltd. “ B. subtilis R31” received a Chinese common name from the National Pesticide Standardization Technical Committee, and the trade name is “Dingwei”.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Biocontrol endophytes Bacillus subtilis R31 influence the quality, transcriptome and metabolome of sweet corn

Shao

Chen²,

Gong³

et al. 2023

PeerJ

Self Cite

View full text Add to dashboard Cite

During colonization of soil and plants, biocontrol bacteria can effectively regulate the physiological metabolism of plants and induce disease resistance. To illustrate the influence of Bacillus subtilis R31 on the quality, transcriptome and metabolome of sweet corn, field studies were conducted at a corn experimental base in Zhuhai City. The results show that, after application of B. subtilis R31, sweet corn was more fruitful, with a 18.3 cm ear length, 5.0 cm ear diameter, 0.4 bald head, 403.9 g fresh weight of single bud, 272.0 g net weight of single ear, and 16.5 kernels sweetness. Combined transcriptomic and metabolomic analyses indicate that differentially expressed genes related to plant-pathogen interactions, MAPK signaling pathway-plant, phenylpropanoid biosynthesis, and flavonoid biosynthesis were significantly enriched. Moreover, the 110 upregulated DAMs were mainly involved in the flavonoid biosynthesis and flavone and flavonol biosynthesis pathways. Our study provides a foundation for investigating the molecular mechanisms by which biocontrol bacteria enhance crop nutrition and taste through biological means or genetic engineering at the molecular level.

show abstract

“…For example, Trichoderma strains can significantly inhibit the spread of banana wilt disease [8]. Bacillus subtilis R31 and TR21 showed beneficial effects on the control of Foc TR4 [9]. A growth promotion of Burkholderia sp.…”

Section: Introductionmentioning

confidence: 99%

Antifungal Mechanism of Metabolites from Newly Isolated Streptomyces sp. Y1-14 against Banana Fusarium Wilt Disease Using Metabolomics

Cao

Cheng

Cai

et al. 2022

JoF

View full text Add to dashboard Cite

Banana Fusarium wilt caused by Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) is one of the most destructive banana diseases in the world, which limits the development of the banana industry. Compared with traditional physical and chemical practices, biological control becomes a promising safe and efficient strategy. In this study, strain Y1-14 with strong antagonistic activity against Foc TR4 was isolated from the rhizosphere soil of a banana plantation, where no disease symptom was detected for more than ten years. The strain was identified as Streptomyces according to the morphological, physiological, and biochemical characteristics and the phylogenetic tree of 16S rRNA. Streptomyces sp. Y1-14 also showed a broad-spectrum antifungal activity against the selected 12 plant pathogenic fungi. Its extracts inhibited the growth and spore germination of Foc TR4 by destroying the integrity of the cell membrane and the ultrastructure of mycelia. Twenty-three compounds were identified by gas chromatography–mass spectrometry (GC-MS). The antifungal mechanism was investigated further by metabolomic analysis. Strain Y1-14 extracts significantly affect the carbohydrate metabolism pathway of Foc TR4 by disrupting energy metabolism.

show abstract

Comparative genomics analysis of two banana Fusarium wilt biocontrol endophytes Bacillus subtilis R31 and TR21 provides insights into their differences on phytobeneficial trait

Cited by 20 publications

References 83 publications

Monitoring Tritrophic Biocontrol Interactions Between Bacillus spp., Fusarium oxysporum f. sp. cubense, Tropical Race 4, and Banana Plants in vivo Based on Fluorescent Transformation System

Monitoring Tritrophic Biocontrol Interactions Between Bacillus spp., Fusarium oxysporum f. sp. cubense, Tropical Race 4, and Banana Plants in vivo Based on Fluorescent Transformation System

Biocontrol endophytes Bacillus subtilis R31 influence the quality, transcriptome and metabolome of sweet corn

Antifungal Mechanism of Metabolites from Newly Isolated Streptomyces sp. Y1-14 against Banana Fusarium Wilt Disease Using Metabolomics

Contact Info

Product

Resources

About