Comparative genomic hybridization reveals a recurrent pattern of chromosomal aberrations in severe dysplasia/carcinoma in situ of the cervix and in advanced-stage cervical carcinoma

Kirchhoff, Maria; Rose, Hanne; Petersen, Bodil Laub; Maahr, Jan; Gerdes, Tommy; Lundsteen, Claes; Bryndorf, Thue; Kryger-Baggesen, Niels; Christensen, Lise; Engelholm, Svend Aage; Philip, John

doi:10.1002/(sici)1098-2264(199902)24:2<144::aid-gcc7>3.0.co;2-9

Cited by 121 publications

(90 citation statements)

References 13 publications

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“…These data support gain of 3q as an important event in the oncogenesis of HPV-related SCCs since, in a previous study, we have shown that a similar proportion of stage 1b HPV-positive cervical SCCs harbour 3q gain (Allen et al, 2000). Gain of 3q has also been detected by others in cervical SCCs (Heselmeyer et al, 1996(Heselmeyer et al, , 1997bDellas et al, 1999;Kirchoff et al, 1999) and in HPV-positive SCC of the anus and its precursor lesions (Heselmeyer et al, 1997a;Haga et al, 2001). Further, we were able to map the smallest region of gain in vulvar SCCs to 3q22 -25, whilst our previous series of cervical cancers showed the smallest area of gain to be 3q24 -26 (Allen et al, 2000).…”

Section: Discussionsupporting

confidence: 87%

“…Furthermore, two of the microsatellite markers showing LOH which were used in the previous studies were mapped to the region of 3p24, which was the smallest Comparative genomic hybridisation in vulvar cancer DG Allen et al region of chromosome loss detected by CGH in the current study. Loss of 3p is also frequently seen in cervical SCC, by CGH and LOH analysis (Heselmeyer et al, 1996(Heselmeyer et al, , 1997bLarson et al, 1997;Wistuba et al, 1997;Kersemaekers et al, 1998;Steenbergen et al, 1998;Dellas et al, 1999;Kirchoff et al, 1999;Allen et al, 2000). Loss of 11q has been documented in cervical and other malignancies not related to HPV infection, including breast, colorectal and ovarian cancers and malignant melanoma (Hampton et al, 1994;Kersemaekers et al, 1998;Allen et al, 2000).…”

Section: Discussionmentioning

confidence: 99%

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Genetic aberrations detected by comparative genomic hybridisation in vulvar cancers

Hammet

et al. 2002

Br J Cancer

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Squamous cell carcinoma of the vulva is a disease of significant clinical importance, which arises in the presence or absence of human papillomavirus. We used comparative genomic hybridisation to document non-random chromosomal gains and losses within human papillomavirus positive and negative vulvar cancers. Gain of 3q was significantly more common in human papillomavirus-positive cancers compared to human papillomavirus-negative cancers. The smallest area of gain was 3q22 -25, a chromosome region which is frequently gained in other human papillomavirus-related cancers. Chromosome 8q was more commonly gained in human papillomavirus-negative compared to human papillomavirus-positive cancers. 8q21 was the smallest region of gain, which has been identified in other, non-human papillomavirus-related cancers. Chromosome arms 3p and 11q were lost in both categories of vulvar cancer. This study has demonstrated chromosome locations important in the development of vulvar squamous cell carcinoma. Additionally, taken together with previous studies of human papillomaviruspositive cancers of other anogenital sites, the data indicate that one or more oncogenes important in the development and progression of human papillomavirus-induced carcinomas are located on 3q. The different genetic changes seen in human papillomavirus-positive and negative vulvar squamous cell carcinomas support the clinicopathological data indicating that these are different cancer types.

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Section: Discussionsupporting

confidence: 87%

Section: Discussionmentioning

confidence: 99%

Genetic aberrations detected by comparative genomic hybridisation in vulvar cancers

Hammet

et al. 2002

Br J Cancer

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“…TERC (3q26) is the RNA component of the human chromosome telomerase, which has been certified to be the most frequently observed amplified oncogene in cervical precancerous lesions (Heselmeyer et al, 1996(Heselmeyer et al, , 1997Kirchhoff et al, 1999;Umayahara et al, 2005). Heselmeyer et al (2003) applied a FISH probe set to the cervical cytological specimens and found that the TERC amplification status was associated with the severity of cervical lesions, and was predictive for the disease regression or progression (Heselmeyer et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

“…We reviewed relevant literature and selected the human telomerase RNA gene TERC (3q26), the most commonly observed amplified oncogene in cervical precancerous lesion, as the biomarker for the cervical lesion diagnoses (Heselmeyer et al, 1997;Kirchhoff et al, 1999;Sokolova et al, 2007;Policht et al, 2010). Fluorescence in situ hybridization (FISH) was conducted to check the TERC amplification in the residual liquidbased cytological specimens, and the high-risk HPV infections were tested by the HC2 method.…”

Section: Significance Of Human Telomerase Rna Gene Amplification Detementioning

confidence: 99%

“…The contrast between the high rate of HPV infection and the low rate of cervical cancer morbidity suggests that additional genetic events occur during malignant progression (Zhang et al, 2002). The oncogene amplification is frequently observed in the cervical precancerous lesion according to the comparative genomic hybridization (CGH) study results (Heselmeyer et al, 1997;Kirchhoff et al, 1999) and is a fairly early event in cervical carcinogenesis.…”

Section: Introductionmentioning

confidence: 99%

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Significance of Human Telomerase RNA Gene Amplification Detection for Cervical Cancer Screening

Chen

Lin²,

Liu³

et al. 2012

Asian Pacific Journal of Cancer Prevention

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Aim: Liquid-based cytology is the most often used method for cervical cancer screening, but it is relatively insensitive and frequently gives equivocal results. Used as a complementary procedure, the high-risk human papillomavirus (HPV) DNA test is highly sensitive but not very specific. The human telomerase RNA gene (TERC) is the most often amplified oncogene that is observed in cervical precancerous lesions. We assessed genomic amplification of TERC in liquid-based cytological specimens to explore the optimal strategy of using this for cervical cancer screening. Methods: Six hundred and seventy-one residual cytological specimens were obtained from outpatients aged 25 to 64 years. The specimens were evaluated by the Digene Hybrid Capture 2 (HC2) HPV DNA test and fluorescence in situ hybridization (FISH) with a chromosome probe to TERC (3q26). Colposcopic examination and histological evaluation were performed where indicated. Results: The TERC positive rate was higher in the CIN2+ (CIN2, CIN3 and SCC) group than in the normal and CIN 1 groups (90.0% vs. 10.4%, p < 0.01). In comparison with the HC2 HPV DNA test, the TERC amplification test had lower sensitivity but higher specificity (90.0% vs. 100.0%, 89.6% vs. 44.0%, respectively). TERC amplification test used in conjunction with the HC2 HPV DNA test showed a combination of 90.0% sensitivity and 92.2% specificity. Conclusion: The TERC amplification test can be used to diagnose cervical precancerous lesions. TERC and HPV DNA co-testing shows an optimal combination of sensitivity and specificity for cervical cancer screening.

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Deletions below 10 megabasepairs are detected in comparative genomic hybridization by standard reference intervals

Kirchhoff¹,

Gerdes²,

Maahr³

et al. 1999

Genes Chromosom. Cancer

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Comparative genomic hybridization reveals a recurrent pattern of chromosomal aberrations in severe dysplasia/carcinoma in situ of the cervix and in advanced-stage cervical carcinoma

Cited by 121 publications

References 13 publications

Genetic aberrations detected by comparative genomic hybridisation in vulvar cancers

Genetic aberrations detected by comparative genomic hybridisation in vulvar cancers

Significance of Human Telomerase RNA Gene Amplification Detection for Cervical Cancer Screening

Deletions below 10 megabasepairs are detected in comparative genomic hybridization by standard reference intervals

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