Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains

Yang, Qian; Dong, Xuan; Xie, Guosi; Fu, Songzhe; Zou, Peizhuo; Sun, Jing; Wang, Yi; Huang, Jie

doi:10.1007/s00438-019-01559-7

Cited by 18 publications

(12 citation statements)

References 57 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The genomes of 123, 137 and HZ56 were sequenced previously Yang et al . (2019) and Fu et al . (2020) by PacBio RSII sequencing platform with the single molecule, real‐time (SMRT) technology (Pacific Biosciences, Menlo Park, CA) at the Novogene China (Tianjin).…”

Section: Methodsmentioning

confidence: 96%

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Yang

Zou

et al. 2020

J. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

Aims Acute hepatopancreatic necrosis disease (AHPND) caused by Vibrio parahaemolyticus has emerged as a severe bacterial disease of cultured shrimp. To identify the key virulence factors, two AHPND‐causing V. parahaemolyticus (VpAHPND) strains (123 and 137) and two non‐VpAHPND strains (HZ56 and ATCC 17082) were selected. Methods and Results Challenge tests showed that the four strains exhibited different virulence towards shrimp with cumulative mortalities at 48 h postinfection (hpi) ranging from 10 to 92%. The expression of pirABVP in strain 123 and 137 was not significantly different. Genomic analysis revealed that the two VpAHPND strains contain a plasmid with the PirABVP toxins (pirABVP) flanked by the insertion sequence (ISVal1) that has been identified in various locations of chromosomes in VpAHPND strains. The two VpAHPND strains possessed almost identical virulence factors, while ISVal1 disrupted three genes related to flagellar motility in strain 137. Phenotype assay showed that strain 123 possessed the highest growth rate and swimming motility, followed by strain 137, suggesting that the disruption of essential genes mediated by ISVal1 significantly affected the virulence level. Transcriptome analysis of two VpAHPND strains (123 and 137) further suggested that virulence genes related to the capsule, flagella and primary metabolism were highly expressed in strain 123. Conclusions Here for the first time, it is demonstrated that the virulence of VpAHPND is not only determined by the expression of pirABVP, but also is mediated by ISVal1 which affects the genes involved in flagellar motility and primary metabolism. Significance and Impact of the Study The genomic and transcriptomic analysis of VpAHPND strains provides valuable information on the virulence factors affecting the pathogenicity of VpAHPND.

show abstract

Section: Methodsmentioning

confidence: 96%

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Yang

Zou

et al. 2020

J. Appl. Microbiol.

Self Cite

View full text Add to dashboard Cite

show abstract

“…The two loci of T6SS present in each chromosome play a role in both inter-bacterial and host–pathogen interactions [ 36 ]. The presence of T6SS among the isolates associated with disease outbreak in this study implies that acquisition of T6SS might offer a fitness advantage for this opportunistic pathogen in out-competing other microbes in the same ecological niche and thereby influencing the colonization outcomes [ 37 ]. Similarly, T3SS’s are involved in the secretion of toxins that cause cytotoxicity and enterotoxicity [ 25 ].…”

Section: Resultsmentioning

confidence: 99%

Whole genome analysis unveils genetic diversity and potential virulence determinants in Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

et al. 2021

View full text Add to dashboard Cite

“…Proteobacteria have dominated the penaeid shrimp gut microbiota, and the microbiome is involved in the regulation of shrimp health and disease [50]. Most studies of P. vannamei affected with AHPND are focused on the bacterial community characterization of the Hp, stomach, intestine or sediment, using 16S rRNA amplicons [51][52][53]. Toxins affect the microbial communities of their host [54], but there are few studies about the effects on the surrounding microbiota of the seawater by the presence of diseased aquatic organisms [55].…”

Section: Changes In the Microbiota Of Seawatermentioning

confidence: 99%

PirAB from <em>Vibrio parahaemolyticus</em> Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in <em>Penaeus vannamei</em>

Soto-Rodríguez¹,

Lozano‐Olvera²,

Montfort³

et al. 2022

Preprint

View full text Add to dashboard Cite

PirAB is a binary protein complex secreted by specific strains of Vibrio parahaemolyticus (Vp) that harbor the pVA1 virulence plasmid and express PirAVp and PirBVp toxins. PirABVp causes acute hepatopancreatic necrosis disease (AHPND), a newly emergent penaeid shrimp disease that can cause 70–100% mortality and has resulted in great economic losses since its appearance. The cy-totoxic effect of PirABVp on the epithelial cell of the shrimp hepatopancreas has been extensively reported. Our studies found that the PirBVp subunit has lectin activity and recognizes mucin-like O-glycosidic structures in the shrimp hepatopancreas. The PirAVp subunit may have a stabiliza-tion function of the binary complex. However, we also found that Vp AHPND changes the water microbiota community structure and causes a significant reduction in several bacteria, especially Neptuniibacter spp. We propose that the PirABvp toxin could exhibit a dual role: damage the shrimp hepatopancreas and kill surrounding bacteria.

show abstract

Comparative genomic analysis unravels the transmission pattern and intra-species divergence of acute hepatopancreatic necrosis disease (AHPND)-causing Vibrio parahaemolyticus strains

Cited by 18 publications

References 57 publications

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Coordination of primary metabolism and virulence factors expression mediates the virulence of Vibrio parahaemolyticus towards cultured shrimp ( Penaeus vannamei )

Whole genome analysis unveils genetic diversity and potential virulence determinants in Vibrio parahaemolyticus associated with disease outbreak among cultured Litopenaeus vannamei (Pacific white shrimp) in India

PirAB from <em>Vibrio parahaemolyticus</em> Exhibits a Binary Toxin Action with Bactericidal Activity and Cytotoxicity in <em>Penaeus vannamei</em>

Contact Info

Product

Resources

About