Comparative Genomic Analysis Reveals Ecological Differentiation in the Genus Carnobacterium

Iskandar, Christelle F.; Borges, Frédéric; Taminiau, Bernard; Daube, Georges; Zagorec, Monique; Remenant, Benoît; Leisner, Jørgen J.; Hansen, Mette Sif; Sørensen, Søren J.; Mangavel, Cécile; Cailliez‐Grimal, Catherine; Revol-Junelles, Anne-Marie

doi:10.3389/fmicb.2017.00357

Cited by 28 publications

(25 citation statements)

References 78 publications

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“…Our network analysis showed the presence of Carnobacterium , Glutamicibacter and Paeniglutamicibacter in GC patients. The genus Carnobacterium encompasses 11 species that have been isolated in cold and temperate environments, from the gastrointestinal tract of animals, and from foods of animal origin, such as seafood, meat and dairy products . Carnobacterium has been isolated from vacuum‐packaged bologna sausage and was responsible for causing green discolouration of the product after package opening .…”

Section: Discussionmentioning

confidence: 99%

Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions

Gantuya

Serag

Matsumoto

et al. 2020

Aliment Pharmacol Ther

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Summary Background Incidence and mortality of gastric cancer (GC) are high in Mongolia despite Helicobacter pylori in the Mongolian population being less virulent. Aim To evaluate gastric bacterial microbiota profiles in patients with GC and its precursor histological conditions. Methods We conducted a case‐control study among 48 GC and 120 noncancer patients (20 normal gastric mucosa [control], 20 gastritis, 40 with atrophy and 40 intestinal metaplasia [IM]). We performed 16S rRNA gene amplicon sequencing and compared taxonomic and functional prediction profiles based on the diagnosis group and H pylori infection status. Results The highest overall bacterial alpha diversity metrics were observed in the control group, followed by the IM and cancer groups. The gastritis and atrophy groups had the least diversity. Lactobacilli and Enterococci were the dominant genus in several cancer patients especially in the absence of H pylori. In addition, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium and Parvimonas were associated with GC regardless of H pylori infection. Firmicutes were decreased in the gastritis and atrophy groups and increased in the IM and cancer groups. The functional metabolic activity of the Embden‐Meyerhof‐Parnas pathway and the utilization of sugar, were significantly increased in cancer group compared with the noncancer group. Conclusion Microbial factors other than H pylori may play a role in Mongolian GC. We identified novel associations between GC and the genera Enterococcus, Lactobacillus, Carnobacterium, Glutamicibacter, Paeniglutamicibacter, Fusobacterium, and Parvimonas.

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Section: Discussionmentioning

confidence: 99%

Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions

Gantuya

Serag

Matsumoto

et al. 2020

Aliment Pharmacol Ther

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“…The genus Enterococcus, which also decreased in these mice, has been implicated in infection and increasing susceptibility to IBD [76]. Carnobacterium sp., which is found in both extreme temperature and animal environments, potentially contains mucin-degrading abilities [77]. The genus Lactococcus is well known for its anti-in ammatory capabilities, has been shown to ameliorate colitis, and also protects the liver from in ammation [56,78].…”

Section: Discussionmentioning

confidence: 99%

Understanding the Physiological Functions of the Host Xenobiotic-sensing Nuclear Receptors PXR and CAR on the Gut Microbiome using Genetically Modified Mice

Little

Dutta

et al. 2020

Preprint

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Background: Pharmacological activation of the host xenobiotic-sensing nuclear receptors pregnane X receptor (PXR) and constitutive androstane receptor (CAR) is well-known to increase drug metabolism and reduce inflammation. Little is known regarding the physiological functions of PXR and CAR on the gut microbiome, which is an important regulator for the host immune surveillance and bile acid (BA) metabolism. We examined the gut microbiome composition and BA metabolites in high vs. low PXR/CAR-expressing mice, and in mice that are deficient in PXR, CAR, or both, at two developmental ages. We also utilized humanized PXR transgenic (hPXR-TG) mice to compare the species-specific effect of PXR on the gut microbiome. Results: We discovered bivalent hormetic functions of PXR and CAR in modulating the richness of the gut microbiome and inflammatory biomarkers: the high PXR/CAR expressers had higher microbial richness, pro-inflammatory bacteria (distinct taxa in Helicobacteraceae and Helicobacter), and fecal pro-inflammatory cytokines, suggesting higher immune surveillance to prevent the colonization of harmful bacteria. Interestingly, the absence of PXR or CAR also increased the microbial richness, and absence of both receptors synergistically increased the microbial richness. PXR and CAR deficiency increased the pro-inflammatory bacteria (Helicobacteraceae and Helicobacter). Most notably, deficiency in both PXR and CAR markedly increased the relative abundance of Lactobacillus, which is capable of bile salt hydrolase (BSH) activity. This corresponded to a decrease in major primary taurine-conjugated bile acids (BAs) in feces, which may lead to higher internal burden of taurine and unconjugated BAs, both of which are linked to inflammation, oxidative stress, and cytotoxicity. hPXR-TG mice had a distinct microbial profile as compared to wild-type mice, including a higher representation of Prevotella. hPXR-TG mice also had higher 12-OH BAs but lower 6-OH BAs, suggesting PXR’s species-specific role in modulating host hepatic BA synthesis. Conclusions: This study is the first to show that the host PXR and CAR profoundly influence the composition of the gut microbiome and its BA metabolites, with a bivalent hormetic relationship between PXR/CAR levels and microbial richness, unveiling the involvement of PXR/CAR-microbiome interactions in host immune surveillance and BA metabolism.

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“…BPA plates allowed the selection of a number of LAB belonging to the species Carnobacterium gallinarum (24%), Enterococcus faecalis (23%), and C. divergens (8%). C. divergens is one of the most prevalent Carnobacterium species in food derived from animals and it is capable to aerobically respire likely using a functional respiratory chain (Iskandar et al, 2017). Carnobacterium was not isolated on MRS anaerobic plates but was found in PCA and BPA plates, also because of its preference for slightly high pH and the sensitivity to acetate (Leisner et al, 2007).…”

Section: Identification Of the Isolates At The End Of The Shelf Lifementioning

confidence: 99%

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

Raimondi

Nappi

Sirangelo

et al. 2018

International Journal of Food Microbiology

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Ten lots of industrial raw sausages in modified atmosphere (CO 30%, O 70%), produced in the same plant over 7 months, were analyzed at the day after production (S samples) and at the end of shelf life (E samples), after 12 days storage at 7 °C to simulate thermal abuse. Quality of the products was generally compromised by storage at 7 °C, with only 3 E samples without alterations. During the shelf life, the pH decreased for the accumulation of acetic and lactic acids. A few biogenic amines accumulated, remaining below acceptable limits. The profile of volatile compounds got enriched with alcohols, ketones, and acids (e.g. ethanol, 2,3-butanediol, 2,3-butandione, butanoic acid) originated by bacterial metabolism. Throughout the shelf life, aerobic bacteria increased from 4.7 log to 6.6 log cfu/g, and lactic acid bacteria (LAB) from 3.7 to 8.1 log cfu/g. Staphylococci, enterobacteria, and pseudomonads passed from 3.7, 3.0, and 1.7 to 5.5, 4.8, and 3.0 log cfu/g, respectively. Dominant cultivable LAB, genotyped by RAPD-PCR, belonged to the species Lactobacillus curvatus/graminis and Lactobacillus sakei, with lower amounts of Leuconostoc carnosum and Leuconostoc mesenteroides. Brochothrix thermosphacta was the prevailing species among aerobic bacteria. The same biotypes ascribed to several different species where often found in E samples of diverse batches, suggesting a recurrent contamination from the plant of production. Profiling of 16S rRNA gene evidenced that microbiota of S samples clustered in two main groups where either Firmicutes or Bacteroidetes prevailed, albeit with taxa generally associated to the gastro-intestinal tract of mammals. The microbial diversity was lower in E samples than in S ones. Even though a common profile could not be identified, most E samples clustered together and were dominated by Firmicutes, with Lactobacillaceae and Listeriaceae as the most abundant families (mostly ascribed to Lactobacillus and Brochothrix, respectively). In a sole E sample Proteobacteria (especially Serratia) was the major phylum.

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Comparative Genomic Analysis Reveals Ecological Differentiation in the Genus Carnobacterium

Cited by 28 publications

References 78 publications

Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions

Gastric mucosal microbiota in a Mongolian population with gastric cancer and precursor conditions

Understanding the Physiological Functions of the Host Xenobiotic-sensing Nuclear Receptors PXR and CAR on the Gut Microbiome using Genetically Modified Mice

Bacterial community of industrial raw sausage packaged in modified atmosphere throughout the shelf life

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