Comparative Genomic Analysis Provides Insights into the Evolution and Genetic Diversity of Community-Genotype Sequence Type 72 Staphylococcus aureus Isolates

Zhou, Wangxiao; Jin, Yue; Zhou, Yanzi; Wang, Yuan; Xiong, Luying; Luo, Qixia; Xiao, Yonghong

doi:10.1128/msystems.00986-21

Cited by 11 publications

(7 citation statements)

References 35 publications

(43 reference statements)

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“…selu was suspected to be undectable due to its uncertain emetic activity in some studies. What’s more, the egc cluster was observed in all CC5, CC9, CC20, CC25, CC72 and ST672, but occasionally present in CC1, all of which (except for ST672) were reported in other previous studies but with various spa types [ 28 – 30 ]. These data suggested that a close connection between the egc cluster and the clonal genetic background.…”

Section: Discussionsupporting

confidence: 64%

Investigation into the prevalence of enterotoxin genes and genetic background of Staphylococcus aureus isolates from retain foods in Hangzhou, China

Chen,

Zhao,

Yang

et al. 2023

BMC Microbiol

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Background Staphylococcus aureus expresses numerous toxins, many of which are strongly believed to be responsible for specific symptoms and even diseases, making it significant in the pathogenesis of human health. Enterotoxins, which are vital toxins, are associated with foodborne illnesses that manifest through symptoms like vomiting and diarrhea. In the present study, 264 S. aureus isolates obtained from various retail foods in Hangzhou, China were further investigated the profiles of enterotoxin genes and genetic backgrounds. Results Approximately, 64.02% of the isolates from diverse sources contained at least one Staphylococcal Enterotoxin (SE) genes, displaying a total of 36 distinct combinations. Enterotoxin gene cluster (egc) encoded enterotoxin genes, normally designated by seg, sei, sem, sen, seo and selu, plus with sep were more frequently detected (33.73%, each). In contrast, see, ses and set were absent in any of the isolates tested. A total of 44 sequence types (STs), 20 clonal complexes (CCs) and 66 different staphylococcal protein A (spa) types (including six novel types) were identified among those 169 SE-positive isolates. Moreover, nineteen methicillin-resistant Staphylococcus aureus (MRSA) isolates were identified. The majority of those isolates belonged to the CC59-Sccmec IVa cluster and carried the seb-sek-seq gene cluster. The egc cluster, either coexisting with or without other enterotoxin genes, was observed in all isolates allocated into CC5, CC9, CC20, CC25, CC72 and ST672. Irrespective of the spa types and origins of the food, it appeared that seh was a distinct genetic element present in isolates belonging to the CC1 clonal lineage. Conclusions The results not only proposed a suspected relationship between distribution of enterotoxigenic strains and genetic backgrounds, but also attributed the presence of novel enterotoxins to potential hazards in food safety.

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Section: Discussionsupporting

confidence: 64%

Investigation into the prevalence of enterotoxin genes and genetic background of Staphylococcus aureus isolates from retain foods in Hangzhou, China

Chen,

Zhao,

Yang

et al. 2023

BMC Microbiol

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“…The other emerging lineages observed in our results belong to ST72 and include the ST72-SCCmecIV and ST72-SCCmecVI. The former is a genetic lineage previously described in community-associated infections in South Korea (45)(46)(47). In contrast, to the best of our knowledge, this is the first description of ST72-SCCmecVI.…”

Section: Discussionmentioning

confidence: 97%

Dynamics of the MRSA Population in A Chilean Hospital: A Phylogenomic Analysis (2000-2016)

Martinez

Planet

Spencer-Sandino

et al. 2023

Preprint

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The global dissemination of methicillin-resistant Staphylococcus aureus (MRSA) is associated with the emergence and establishment of clones in specific geographic areas. The Chilean-Cordobes clone (ChC) (ST5-SCCmecI) has been the predominant MRSA clone in Chile since its first description in 1998, despite the report of other emerging MRSA clones in the last years. Here, we characterize the evolutionary history of MRSA from 2000 to 2016 in a Chilean tertiary healthcare center using phylogenomic analyses. We sequenced 469 MRSA isolates collected between 2000-2016 in a tertiary healthcare center in Chile. We evaluated the temporal trends of the circulating clones and performed a phylogenomic reconstruction to characterize the clonal dynamics. We found a significant increase in the diversity and richness of sequence types (STs; Spearman r=0.8748, p<0.0001) with a Shannon diversity index increasing from 0.221 in the year 2000 to 1.33 in 2016. The temporal trend analysis revealed that in the period 2000-2003 most of the isolates (94.2%; n=98) belonged to the ChC clone. However, since then, the frequency of the ChC clone has decreased over time, accounting for 52% of the collection in the 2013-2016 period. This decline was accompanied by the rise of two emerging MRSA lineages, ST105-SCCmecII and ST72-SCCmecVI. In conclusion, the ChC clone remains the most frequent MRSA lineage in Chile. However, this lineage is gradually being replaced by several emerging clones, the most important of which is clone ST105-SCCmecII. To the best of our knowledge, this is the largest study of MRSA clonal dynamics performed in South America.

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“…ST188 is the major lineage causing multiple host species infections in Shanghai, China ( Wang et al, 2018 ). Furthermore, both international transmission of ST72 isolates and interregional transmission within China were observed, with t148 being the most common spa typing ( Zhou et al, 2021 ). Although ST243 has fewer reports, it may colonize patient-to-contaminated healthcare worker transmission in hospital settings ( Ferreira et al, 2013 ).…”

Section: Discussionmentioning

confidence: 99%

Prevalence, antibiotic resistance and molecular characterization of Staphylococcus aureus in ready-to-eat fruits and vegetables in Shanghai, China

Jia,

Qin,

et al. 2024

Current Research in Food Science

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Comparative Genomic Analysis Provides Insights into the Evolution and Genetic Diversity of Community-Genotype Sequence Type 72 Staphylococcus aureus Isolates

Cited by 11 publications

References 35 publications

Investigation into the prevalence of enterotoxin genes and genetic background of Staphylococcus aureus isolates from retain foods in Hangzhou, China

Investigation into the prevalence of enterotoxin genes and genetic background of Staphylococcus aureus isolates from retain foods in Hangzhou, China

Dynamics of the MRSA Population in A Chilean Hospital: A Phylogenomic Analysis (2000-2016)

Prevalence, antibiotic resistance and molecular characterization of Staphylococcus aureus in ready-to-eat fruits and vegetables in Shanghai, China

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