Comparative genomic analysis of five pairs of virulent parental/attenuated vaccine strains of PRRSV

An, Tong Qing; Tian, Zhen; Zhou, Yanjun; Xiao, Yan; Peng, Jinmei; Chen, Jin; Jiang, Yifeng; Hao, Xiao-Fang; Tong, Guangzhi

doi:10.1016/j.vetmic.2010.11.001

Cited by 44 publications

(39 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…To date, a number of type 2 PRRS MLV vaccines have been successfully employed in the global market. In addition, live attenuated candidate vaccines have been exploited by prolonged viral passages of the emerged HP-PRRSV isolates on MARC-145 cells and domestically implemented in China (An et al, 2011a;Han et al, 2009;Leng et al, 2012a,b;Tian et al, 2009). In the present study, we developed a PRRSV MLV vaccine candidate obtained by 100 passages of the virulent MN184-related nsp2 DEL strain CA-2 in MARC-145 cells and ascertained the pathogenic and genetic characteristics associated with the cell adaptation process.…”

Section: Discussionmentioning

confidence: 99%

“…Indeed, the first type 2 live vaccine, Ingelvac PRRS MLV, was obtained by continuous passages in MARC-145 cells (Opriessnig et al, 2002). More recently, live attenuated HP-PRRSV vaccines were developed by in vitro serial passages in MARC-145 cells and are now available in Chinese markets, showing effectiveness and protection by reducing the economic loss against PRRS (An et al, 2011a;Han et al, 2009;Leng et al, 2012a,b;Tian et al, 2009). In the present study, the virulent Korean PRRSV nsp2 DEL strain CA2 was culturally passaged 100 times in MARC-145 cells for attenuation so that it could be further used for the development of an MLV vaccine.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Pathogenicity and genetic characteristics associated with cell adaptation of a virulent porcine reproductive and respiratory syndrome virus nsp2 DEL strain CA-2

Lee

Choi²,

Nam³

et al. 2016

Veterinary Microbiology

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Pathogenicity and genetic characteristics associated with cell adaptation of a virulent porcine reproductive and respiratory syndrome virus nsp2 DEL strain CA-2

Lee

Choi²,

Nam³

et al. 2016

Veterinary Microbiology

View full text Add to dashboard Cite

“…Mutations associated with PRRSV attenuation had been revealed in several studies; the investigators suggested that multiplate regions would relate to the attenuation of PRRSV [18, 24, 30–33]. In this study, a highly pathogenic PRRSV strain named QY1 P5 was passaged in vitro, and mutations occurred during this process, including 34 amino acid changes, 1 nucleotide deletion, and 34 amino acid deletions in the QY1 P100.…”

Section: Discussionmentioning

confidence: 93%

Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein

Sun

et al. 2014

Journal of Immunology Research

View full text Add to dashboard Cite

A porcine reproductive and respiratory syndrome virus (PRRSV) QY1 was serially passed on Marc-145 cells. Virulence of different intermediate derivatives of QY1 (P5, P60, P80, and P100) were determined. The study found that QY1 had been gradually attenuated during the in vitro process. Pathogenicity study showed that pigs inoculated with QY1 P100 and P80 did not develop any significant PRRS clinic symptoms. However, mild-to-moderate clinical signs and acute HP-PRRSV symptoms of infection were observed in pigs inoculated with QY1 P60 and P5, respectively. Furthermore, we determined the whole genome sequences of these four intermediate viruses. The results showed that after 100 passages, compared to QY1 P5, a total of 32 amino acid mutations were found. Moreover, there were one nucleotide deletion and a unique 34-amino acid deletion found at 5′UTR and in nsp2 gene during the attenuation process, respectively. Such deletions were genetically stable in vivo. Following PRRSV experimental challenge, pigs inoculated with a single dose of QY1 P100 developed no significant clinic symptoms and well tolerated lethal challenge, while QY1 P80 group still developed mild fever in the clinic trial after challenge. Thus, we concluded that QY1 P100 was a promising and highly attenuated PRRSV vaccine candidate.

show abstract

“…In the recent years, new NA type PRRSV variants called highly pathogenic strains (HP) has emerged in Vietnam and China causing large-scale outbreaks and destructive clinical syndromes [6,8] . Those HP-PRRSV isolates share high sequence identity and have similar deletions/mutations in difference regions of viral genome such as two deletions in non-structural protein 2, one deletion in the 5'-untranslated region, and one deletion in the 3' untranslated region, and some other point mutations [9] . Recently, several studies on genetic variation and phylogenetic relationship based on major structural genes of PRRSV isolates such as ORF5, ORF7 have been done [10][11][12][13][14] .…”

Section: Introductionmentioning

confidence: 99%

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Thúy¹,

Thu²,

Son³

et al. 2015

Kafkas Univ Vet Fak Derg

View full text Add to dashboard Cite

Porcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating diseases for the swine industry worldwide. The 372-bp complete nuclear capsid protein (N-protein) encoding gene (ORF7) of 36 field PRRSV isolates from Vietnam collected during 2008-2012 were sequenced and compared with certain vaccines and published strains of PRRSV. The ORF7 nucleotide sequence (nt) similarity and amino acid (aa) identity among 36 strains showed the highest and ranged from 95.1 to 100%. These isolates shared similarities with VR-2332 (nt 91.9-94.3%, aa 92.6-96.7%) and LV (nt 62.7-65.3%, aa 58.5-60.1%). There were higher level of similarity with QN07 (nt 96.2-99.1%, aa 96.7-99.1%) from the 2007 PRRS outbreak in Quang Nam province, CH-1a (nt 93.0-96.2%, aa 92.6-95.9%) isolated in China in 1995 and JXA1 (nt 96.7-99.7%, aa 97.5-100%), the highly pathogenic strain from China isolated in 2006. Six aa mutations located in both variable and conserved regions of N-protein amino acid sequence were detected in most of the 36 isolates and highly pathogenic PRRSV strains in China in comparison to the prototype strain VR-2332. Results of sequence analyses indicated that PRRSVs isolated in Vietnam during 2008-2012 were classified as North American genotype. Phylogenetic tree also clustered those 36 PRRSV isolates, other recently reported Vietnamese strains, highly pathogenic Chinese strains and JXA1-R vaccine strain in the same cluster and separated from the prototype strain (VR-2332) and Ingelvac MLV/Besta vaccine strains. The result on genetic characterization of ORF7 of circulating PRRSV strains may assist the development of effective strategies for monitoring and controlling PRRS in Vietnam. Keywords: Genetic variation of ORF7, North American genotype, Phylogenetic analysis, PRRSV Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni'nin Genetik Analizi

show abstract

Comparative genomic analysis of five pairs of virulent parental/attenuated vaccine strains of PRRSV

Cited by 44 publications

References 34 publications

Pathogenicity and genetic characteristics associated with cell adaptation of a virulent porcine reproductive and respiratory syndrome virus nsp2 DEL strain CA-2

Pathogenicity and genetic characteristics associated with cell adaptation of a virulent porcine reproductive and respiratory syndrome virus nsp2 DEL strain CA-2

Attenuation and Immunogenicity of a Live High Pathogenic PRRSV Vaccine Candidate with a 32-Amino Acid Deletion in the nsp2 Protein

Vietnam Domuz Reprodüktif ve Respiratorik Sendrom Virüsü (DRRSV) ORF7 Geni’nin Genetik Analizi

Contact Info

Product

Resources

About