Comparative genome analysis reveals extensive conservation of genome organisation for Arabidopsis thaliana and Capsella rubella

Abstract

SummaryGenome colinearity has been studied for two closely related diploid species of the Brassicaceae family, Arabidopsis thaliana and Capsella rubella. Markers mapping to chromosome 4 of A. thaliana were found on two linkage groups in Capsella and colinear segments spanning more than 10 cM were revealed. Detailed analysis of a 60 kbp region in A. thaliana and its counterpart in C. rubella showed virtually complete conservation of gene repertoire, order and orientation. The comparison of orthologous genes revealed very similar exon±intron structures and sequence identities of 90% or more were found for exon sequences. This extensive genome colinearity at the genetic and molecular level allows the ef®cient transfer of data from the well-studied A. thaliana genome to other species in the Brassicaceae family, substantially facilitating genome analysis studies for species of this family.

Evolution of the self-incompatibility system in the Brassicaceae: identification of S-locus receptor kinase (SRK) in self-incompatible Capsella grandiflora

“…The lineages of Brassica and Arabidopsis/Capsella were estimated to have separated approximately 12.2-19.5 million years ago, whereas for Arabidopsis and Capsella, the estimate is of 6.2-9.8 million years (Acarkan et al, 2000).…”

“…Capsella appears to be closely related to Arabidopsis (Koch et al, , 2001 on the basis of sequence data from various nuclear genes and cpDNA. Comparative genome analysis has revealed extensive conservation of genome organisation among Arabidopsis thaliana and C. rubella (Acarkan et al, 2000;Koch and Kiefer, 2005). Owing to this close relationship it should be possible to make use of the present knowledge of Arabidopsis SI in research on Capsella.…”

Comparative Sequence Analysis Reveals Extensive Microcolinearity in the Lateral Suppressor Regions of the Tomato, Arabidopsis, and Capsella Genomes

“…Comparing the genetic maps of Arabidopsis and different Brassica species also has revealed many colinear chromosome segments for species belonging to the Brassicaceae family (reviewed in Schmidt, 2000). The results of the first microsynteny studies using sequencelevel resolution in the Poaceae (Chen et al, 1997;Messing and Llaca, 1998;Tikhonov et al, 1999) and Brassicaceae families (Grant et al, 1998;Acarkan et al, 2000) support the view that genome colinearity can be observed at the level of genes.…”

“…Comparing the genetic maps of Arabidopsis and different Brassica species also has revealed many colinear chromosome segments for species belonging to the Brassicaceae family (reviewed in Schmidt, 2000). The results of the first microsynteny studies using sequencelevel resolution in the Poaceae (Chen et al, 1997;Messing and Llaca, 1998;Tikhonov et al, 1999) and Brassicaceae families (Grant et al, 1998;Acarkan et al, 2000) support the view that genome colinearity can be observed at the level of genes.Few attempts to analyze genome colinearity between more distantly related species have been reported (Paterson et al, 1996;Devos et al, 1999;van Dodeweerd et al, 1999;Ku et al, 2000). The low degree of sequence homology in distantly related species hampers the unambiguous 1 These authors contributed equally to this work.…”

Papaver rhoeas S-Determinants and the Signaling Networks They Trigger

“…Until now, it was thought that transfer of SI would be limited to close relatives, as so far the only movement of SI systems has been within species or closely related species, Arabidopsis lyrata and Capsella grandifl ora into A. thaliana (Nasrallah et al 2002 ;Boggs et al 2009 ). Although these are important demonstrations, they diverged only ~5 million , and ~6.2-9.8 million years ago (Acarkan et al 2000 ), respectively, and so do not provide major insights into the evolution of SI signaling across angiosperm families because of their close relationship and their possession of a mechanistically common SI system. Our fi ndings provide a breakthrough in this area, as transferral of the Papaver pollen S -determinant into A. thaliana is between highly diverged species with ~144 million years separating them (Bell et al 2010 ), and they do not share a common SI system.…”