Comparative genome analysis of the first <i>Listeria monocytogenes</i> core genome multi-locus sequence types CT2050 AND CT2051 strains with their close relatives

Abstract

&lt;abstract&gt; &lt;p&gt;Genome sequences of the three strains of &lt;italic&gt;L. monocytogenes&lt;/italic&gt;, which are the first core genome multi-locus sequence types (cgMLST) 2050 and 2051 were reviewed and compared with 21 close relatives and reference genomes. Using a pan-genomic approach to analyse whole genome sequences, it was found that the strains consisted of approximately 2200 shared genes and a much greater pool of genes present as an accessory genome. An unknown transmissible sequence of approximately 91 kb harbouring bacitracin resistance genes found in strain LmNG2 (1/2b) was revealed to be an Inc 18 plasmid. The cg2051, strain LmNG3 (1/2a) haboured more unique genes (252 vs 230) than the well-known reference strain LmEGD-e (1/2a). More studies to monitor new strains can help reduce food-borne outbreaks.&lt;/p&gt; &lt;/abstract&gt;

Effect of TraN key residues involved in DNA binding on pIP501 transfer rates in Enterococcus faecalis

“…Furthermore, plasmids were not exclusively found to belong to the Inc18 group but also to RepA_N and Rep_3 replicon families, underlining the widespread presence of pIP501-like T4SS. Most TraN homologs were found on multiple resistance Inc18 plasmids known to disseminate to a wide range of Gram-positive hosts ( Kohler et al, 2018b ; Nwaiwu, 2022 ; Zhu et al, 2022 ). Inc18 plasmids have been found in diverse Firmicutes isolated from food, healthcare facilities, livestock manure, or the environment ( Biggel et al, 2021 ; Parra-Flores et al, 2021 ; Jozefíková et al, 2022 ; Tang et al, 2023 ).…”