Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations

Knell, Sebastian; Willems, Hermann; Hertrampf, B.; Reiner, Gerald

doi:10.1016/j.vetmic.2005.06.004

Cited by 48 publications

(37 citation statements)

References 17 publications

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“…of Csagola et al (2006), Knell et al (2005), and Cadar et al (2010) the tissues analyzed were only spleen in the study by Knell, and kidneys, liver, spleen, and lymph nodes in two other studies. In the study by Reiner et al (2010), tonsils were included in the analysis, as well as lungs, spleen, and lymph nodes.…”

Section: Discussionmentioning

confidence: 98%

“…In Hungary, Csagola et al (2006) analyzed 307 organ samples and found 20.5% positive for PCV2 DNA. In Germany, Knell et al (2005) tested 238 samples of spleen from wild boars and 43 (18.1%) were positive. Also Reiner et al (2010) analyzed 349 samples from German wild boars and found 63.1% (by standard PCR) and 45.4% (real-time PCR) PCV2 positive.…”

Section: Discussionmentioning

confidence: 99%

“…Increasing prevalence of PCV2 in wild boars also can be suspected in at least some states of Germany. Knell et al (2005) The differences between the various countries in prevalence of PCV2-positive wild boars may indicate true variation in the virus' distribution in wild boar populations or may be because different tissues were used for the analyses. In the studies FIGURE 1.…”

Section: Discussionmentioning

confidence: 99%

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Prevalence of Infection and Genetic Diversity of Porcine Circovirus Type 2 (Pcv2) in Wild Boar (Sus Scrofa) in Poland

Fabisiak

Szczotka

Podgórska

et al. 2012

Journal of Wildlife Diseases

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ABSTRACT:Porcine circovirus type 2 (PCV2) is a widespread, important pathogen of domestic swine and the causative agent of postweaning multisystemic wasting syndrome and other diseases and conditions referred to as ''porcine circovirus diseases.'' Specific antibodies and DNA to PCV2 have also been detected in European wild boars and North American feral pigs. We collected 312 tonsil samples from wild boars shot in 13 of 16 districts of Poland, and tested them for PCV2 DNA using a real-time PCR. We detected PCV2 DNA in 75.6% of tested tonsils, and in particular, in 60% of samples from the 2006-07 season, and 91% from 2007-08. The phylogenetic analysis that included 12 PCV2 sequences from wild boars revealed that they belonged to two genetic clusters, PCV2b and PCV2a. We present data on prevalence of PCV2 in Polish wild boars and for the first time report the PCV2a genotype in Poland.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Prevalence of Infection and Genetic Diversity of Porcine Circovirus Type 2 (Pcv2) in Wild Boar (Sus Scrofa) in Poland

Fabisiak

Szczotka

Podgórska

et al. 2012

Journal of Wildlife Diseases

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“…Wild boars (Sus scrofa) infected with PCV2 have been reported (Vicente et al, 2004;Knell et al, 2005;Csagola et al, 2006). A PCV2-antibody prevalence of 47% has been reported for wild boar sampled in Spain; antibody prevalence was highest in intensively managed populations rather than in those living in more natural conditions (Vicente et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Genomic sequences of PCV2 isolates from domestic pigs, with or without PMWS, are closely related (De Boisseson et al, 2004). A number of PCV2 strains have been genetically characterized from wild boars with subclinical infection (Knell et al, 2005;Csagola et al, 2006), but only one has been characterized from a PMWS case (Schulze et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

Detection and Genetic Characterization of Porcine Circovirus 2 Isolates From the First Cases of Postweaning Multisystemic and Wasting Syndrome in Wild Boars in Greece

Sofia

Billinis

Psychas

et al. 2008

Journal of Wildlife Diseases

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ABSTRACT:In 2002, postweaning multisystemic wasting syndrome (PMWS) was diagnosed in a European female wild boar (Sus scrofa), based on the detection of porcine circovirus 2 (PCV2) DNA in various organs, including the uterus, and on histopathologic lesions. This is the first detection of PCV2 DNA in the uterus of a wild boar. Three years later (2005), a wild boar ,6-8 mo of age was found moribund. It presented wasting and dyspnea and finally died. PCV2 DNA was detected in tissue samples, and histopathologic lesions consistent with PMWS were observed. Both wild boars were from neighboring hunting areas in central Greece. Two PCV2 strains from the wild boars were genetically characterized and compared to other reported PCV2 sequences from wild boars and domestic pigs. The PCV-2 sequences from the wild boars in this study were closely related to each other and were grouped with two isolates from wild boars from Hungary. The phylogenetic analysis revealed that the virus might be transmitted between hunting areas. In addition, PCV2 may spread from domestic pigs to wild boars and vice versa.

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Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia

Božidar¹,

Milićević²,

Jakic-Dimic³

et al. 2011

Arch Virol

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Porcine circovirus type 2 (PCV2) is the main causative agent of postweaning multisystemic wasting syndrome (PMWS). To characterize and determine the genetic diversity of PCV2 in the porcine population of Serbia, nucleotide and deduced amino acid sequences of the open reading frame 2 (ORF2) of PCV2 collected from the tissues of pigs that either had died as a result of PMWS or did not exhibit disease symptoms were analyzed. Sequencing and phylogenetic analysis showed considerable diversity among PCV2 ORF2 sequences and the existence of two main PCV2 genotypes, PCV2b and PCV2a, with at least three clusters, 1A/B, 1C and 2D. In order to provide further proof that the 1C strain is circulating in the porcine population, the whole viral genome of one PCV2 isolate was sequenced. Genotyping and phylogenetic analysis using the entire viral genome sequences confirmed that there was a PMWS-associated 1C strain emerging in Serbia. Our analysis also showed that PCV2b is dominant in the porcine population, and that it is exclusively associated with PMWS occurrences in the country. These data constitute a useful basis for further epidemiological studies regarding the heterogeneity of PCV2 strains on the European continent.

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Comparative genetic characterization of Porcine Circovirus type 2 samples from German wild boar populations

Cited by 48 publications

References 17 publications

Prevalence of Infection and Genetic Diversity of Porcine Circovirus Type 2 (Pcv2) in Wild Boar (Sus Scrofa) in Poland

Prevalence of Infection and Genetic Diversity of Porcine Circovirus Type 2 (Pcv2) in Wild Boar (Sus Scrofa) in Poland

Detection and Genetic Characterization of Porcine Circovirus 2 Isolates From the First Cases of Postweaning Multisystemic and Wasting Syndrome in Wild Boars in Greece

Genetic characterization and phylogenetic analysis of porcine circovirus type 2 (PCV2) in Serbia

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