Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells

Sanchez, Maria Carmen Arroyo; Romero-Lastra, Patricia; Ribeiro‐Vidal, Honorato; Llama‐Palacios, Arancha; Figuero, Elena; Herrera, David; Sanz, Mariano

doi:10.1186/s12866-019-1423-9

Cited by 30 publications

(26 citation statements)

References 60 publications

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“…Although the majority of the microorganisms that colonise the oral cavity and dental surfaces are compatible with periodontal health [2], there are specific pathobionts that have shown pathogenicity by disrupting the host immune tolerance and causing a chronic unresolved inflammation in the periodontal tissues, leading to destructive changes in the connective and bone tissue metabolism [3,4]. In particular, Porphyromonas gingivalis has been identified as an example of keystone pathogen, with the capacity to augment the virulence of the entire microbial community through specific inter-bacterial interactions, a characteristic feature of the "biofilm quorum sensing" [5,6], and the expression of certain molecules acting as virulence factors, like proteolytic enzymes or other pro-inflammatory molecules, that will induce a dysbiosis state by modifying the biofilm towards a pro-inflammophilic environment, thus promoting a non-resolving chronic inflammatory host response, what is characteristic of the subgingival biofilm in periodontitis. It should also be taken in consideration that differences intrinsic to the host response of each individual might influence the establishment and progression of the disease [7,8].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model

et al. 2020

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In search for natural products with antimicrobial properties for use in the prevention and treatment of periodontitis, the purpose of this investigation was to evaluate the antimicrobial activity of two omega-3 fatty acids, docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), using an in vitro multi-species subgingival biofilm model including Streptococcus oralis, Actinomyces naeslundii, Veillonella parvula, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans. The antimicrobial activities of EPA and DHA extracts (100 µM) and the respective controls were assessed on 72 h biofilms by their submersion onto discs for 60 s. Antimicrobial activity was evaluated by quantitative polymerase chain reaction (qPCR), confocal laser scanning microscopy (CLSM) and scanning electron microscopy (SEM). ANOVA with Bonferroni correction was used to evaluate the antimicrobial activity of each of the fatty acids. Both DHA and EPA significantly reduced (p < 0.001 in all cases) the bacterial strains used in this biofilm model. The results with CLSM were consistent with those reported with qPCR. Structural damage was evidenced by SEM in some of the observed bacteria. It was concluded that both DHA and EPA have significant antimicrobial activity against the six bacterial species included in this biofilm model.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model

et al. 2020

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“…To evaluate the mechanism by which TCDs inhibit the formation of P. gingivalis biofilm, the key genes involved in biofilm formation by P. gingivalis, namely FimA, RgpA, RgpB and KGP, were investigated. 45,51,52 Gel assays were used to investigate the mRNA expression of these genes ( 16s rRNA served as the internal control gene). Overnightcultured P. gingivalis (1×10 9 CFU/mL) was diluted 10 times as a bacterial suspension, and seeded in two polystyrene flat-bottomed six-well plates at 100 μL per well.…”

Section: Gene Expression Of Biofilm-related Genesmentioning

confidence: 99%

<p>Specific Anti-biofilm Activity of Carbon Quantum Dots by Destroying <em>P. gingivalis</em> Biofilm Related Genes</p>

Liang¹,

Shi²,

Qi³

et al. 2020

IJN

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Introduction: Biofilms protect bacteria from antibiotics and this can produce drug-resistant strains, especially the main pathogen of periodontitis, Porphyromonas gingivalis. Carbon quantum dots with various biomedical properties are considered to have great application potential in antibacterial and anti-biofilm treatment. Methods: Tinidazole carbon quantum dots (TCDs) and metronidazole carbon quantum dots (MCDs) were prepared by a hydrothermal method with the clinical antibacterial drugs tinidazole and metronidazole, respectively. Then, TCDs and MCDs were characterized by transmission electron microscopy, UV-visible spectroscopy, infrared spectroscopy and energy-dispersive spectrometry. The antibacterial effects were also investigated under different conditions. Results: The TCDs and MCDs had uniform sizes. The results of UV-visible and energydispersive spectrometry confirmed their important carbon polymerization structures and the activity of the nitro group, which had an evident inhibitory effect on P. gingivalis, but almost no effect on other bacteria, including Escherichia coli, Staphylococcus aureus and Prevotella nigrescens. Importantly, the TCDs could penetrate the biofilms to further effectively inhibit the growth of P. gingivalis under the biofilms. Furthermore, it was found that the antibacterial effect of TCDs lies in its ability to impair toxicity by inhibiting the major virulence factors and related genes involved in the biofilm formation of P. gingivalis, thus affecting the selfassembly of biofilm-related proteins. Conclusion: The findings demonstrate a promising new method for improving the efficiency of periodontitis treatment by penetrating the P. gingivalis biofilm with preparations of nano-level antibacterial drugs.

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“…In order to evaluate the mechanism of TCDs to interfere the formation of P. gingivalis biofilm, the key genes involved in biofilm formation by P. gingivalis, including FimA, RgpA, RgpB and KGP were investigated [48,54,55]. Gel assay was used to quantify mRNA expression levels of these genes.…”

Section: Gene Expression Of Biofilm Related Genementioning

confidence: 99%

Specific Antibiofilm of Carbon Quantum Dots by Destroying P. gingivalis Biofilm Related Proteins Self-assembly

Liang¹,

Shi

et al. 2020

Preprint

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Biofilms protected bacteria from antibiotics and produced drug-resistant strains, especially the main pathogen of periodontitis- Porphyromonas gingivalis ( P. gingivalis ). In this investigation, the carbon quantum dots (TCDs and MCDs) were prepared by hydrothermal method with clinic antibacterial drug-Tinidazole and Metronidazole and exhibited unique antibiofilm properties. The transmission electron microscope (TEM) images showed that the TCDs and MCDs had uniform sizes and the results of UV-vis and energy dispersive spectrometer (EDS) confirmed their important carbon polymerization structures. The prepared TCDs and MCDs were characterized by infrared spectroscopy and still kept the activity of nitro group, which still had an obvious effect on P. gingivalis . Interestingly, the results showed that they both have significant antibacterial effect on P. gingivalis , while the effect of TCDs was better than MCDs. However the TCDs has almost ineffect on other bacteria including Escherichia coli ( E. coli ), Staphylococcus aureus ( S. aureus ) and Prevotella nigrescens ( P. nigrescens ), the MCDs still have obviously antibacterial ability to P. ningrescens . Importantly, the TCDs can penetrate the biofilms to further effectively inhibited the growth of P. gingivalis under the biofilms. Furtherly, we revealed the hydrophilic TCDs destroy P. gingivalis ’ adhesion and impair toxicity by inhibiting the major virulence factors and related genes involved in biofilm formation of P. gingivalis, thus affect the self-assembly of biofilm related proteins. The findings may not only develop a new promising method for improve the periodontitis treatment efficiency by penetrating P. gingivalis biofilm with preparation of nano-level antibacterial drugs.

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Comparative gene expression analysis of planktonic Porphyromonas gingivalis ATCC 33277 in the presence of a growing biofilm versus planktonic cells

Cited by 30 publications

References 60 publications

Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model

Antimicrobial Activity of EPA and DHA against Oral Pathogenic Bacteria Using an In Vitro Multi-Species Subgingival Biofilm Model

<p>Specific Anti-biofilm Activity of Carbon Quantum Dots by Destroying <em>P. gingivalis</em> Biofilm Related Genes</p>

Specific Antibiofilm of Carbon Quantum Dots by Destroying P. gingivalis Biofilm Related Proteins Self-assembly

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