Comparative foliar metabolomics of a tropical and a temperate forest community

Sedio, Brian E.; Parker, John D.; McMahon, Sean M.; Wright, S. Joseph

doi:10.1002/ecy.2533

Cited by 51 publications

(69 citation statements)

References 32 publications

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“…Psychotria leaf tissues were analyzed using a Bruker maXis Impact quadrupole‐time‐of‐flight mass spectrometer (Bruker Daltonics, Billerica, Massachusetts, USA); otherwise methods were identical to those described by Sedio et al. (, , ) and also applied to Theobroma cacao and Colletotrichum (below). Briefly, three individuals of each focal species of Psychotria were sampled on Barro Colorado Island, Panama, between June and October 2010.…”

Section: Methodsmentioning

confidence: 99%

“…We examined metabolomic differences among the four species of Psychotria used in Experiment 1 (P. gracilenta, P. capitata, P. acuminata, and P. marginata) and a fifth species of Psychotria, P. horizontalis. Psychotria leaf tissues were analyzed using a Bruker maXis Impact quadrupole-time-of-flight mass spectrometer (Bruker Daltonics, Billerica, Massachusetts, USA); otherwise methods were identical to those described by Sedio et al (2017Sedio et al ( , 2018aSedio et al ( , 2018b and also applied to Theobroma cacao and Colletotrichum (below). Briefly, three individuals of each focal species of Psychotria were sampled on Barro Colorado Island, Panama, between June and October 2010.…”

Section: Experiments 2: Metabolomic Differences Among Psychotria Sppmentioning

confidence: 99%

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Host affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistry

Christian

Sedio

Florez‐Buitrago

et al. 2020

American J of Botany

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Premise Interactions between fungal endophytes and their host plants present useful systems for identifying important factors affecting assembly of host‐associated microbiomes. Here we investigated the role of secondary chemistry in mediating host affinity of asymptomatic foliar endophytic fungi using Psychotria spp. and Theobroma cacao (cacao) as hosts. Methods First, we surveyed endophytic communities in Psychotria species in a natural common garden using culture‐based methods. Then we compared differences in endophytic community composition with differences in foliar secondary chemistry in the same host species, determined by liquid chromatography–tandem mass spectrometry. Finally, we tested how inoculation with live and heat‐killed endophytes affected the cacao chemical profile. Results Despite sharing a common environment and source pool for endophyte spores, different Psychotria host species harbored strikingly different endophytic communities that reflected intrinsic differences in their leaf chemical profiles. In T. cacao, inoculation with live and heat‐killed endophytes produced distinct cacao chemical profiles not found in uninoculated plants or pure fungal cultures, suggesting that endophytes, like pathogens, induce changes in secondary chemical profiles of their host plant. Conclusions Collectively our results suggest at least two potential processes: (1) Plant secondary chemistry influences assembly and composition of fungal endophytic communities, and (2) host colonization by endophytes subsequently induces changes in the host chemical landscape. We propose a series of testable predictions based on the possibility that reciprocal chemical interactions are a general property of plant–endophyte interactions.

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Section: Methodsmentioning

confidence: 99%

Section: Experiments 2: Metabolomic Differences Among Psychotria Sppmentioning

confidence: 99%

Host affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistry

Christian

Sedio

Florez‐Buitrago

et al. 2020

American J of Botany

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“…(e) Cumulative compound richness as a function of the number of species examined within four plant genera: iridoid glycosides in Lonicera (Caprifoliaceae) (Lieurance et al ), cardenolides in Asclepias (Apocynaceae) (Rasmann & Agrawal ), leaf volatiles in Quercus (Fagaceae) (Pearse et al ) and floral volatiles in Lithophragma (Saxifragaceae) (Friberg et al ). (f) Cumulative richness of the leaf metabolome as a function of number of tree species examined at Barro Colorado Island, Panama or the Smithsonian Environmental Research Center, Maryland, USA (Sedio et al ).…”

Section: Frames Of Reference: Diversity Needs a Denominatormentioning

confidence: 99%

“…Abundance units are peak area, μg mg ‐1 dry mass and % dry mass. (d) Phytochemical richness and abundance (peak area) across tree species within communities at Barro Colorado Island, Panama or the Smithsonian Environmental Research Center, Maryland, USA (Sedio et al ).…”

Section: Frames Of Reference: Diversity Needs a Denominatormentioning

confidence: 99%

The many dimensions of phytochemical diversity: linking theory to practice

2019

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Research on the ecological and evolutionary roles of phytochemicals has recently progressed from studying single compounds to examining chemical diversity itself. A key conceptual advance enabling this progression is the use of species diversity metrics for quantifying phytochemical diversity. In this perspective, we extend the theory developed for species diversity to further our understanding of what exactly phytochemical diversity is and how its many dimensions impact ecological and evolutionary processes. First, we discuss the major dimensions of phytochemical diversity – richness, evenness, functional diversity, and alpha, gamma and beta diversity. We describe their potential independent roles in biotic interactions and the practical challenges associated with their analysis. Second, we re‐analyse the published and unpublished datasets to reveal that the phytochemical diversity experienced by an organism (or observed by a researcher) depends strongly on the scale of the interaction and the total amount of phytochemicals involved. We argue that we must account for these frames of reference to meaningfully understand diversity. Moving from a general notion of phytochemical diversity as a single measure to a precise definition of its multidimensional and multiscale nature yields overlooked testable predictions that will facilitate novel insights about the evolutionary ecology of plant biotic interactions.

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“…Recent advances in metabolomics may allow large‐scale community comparisons of chemical defense (Sedio et al. ), though defensive function remains difficult to demonstrate across many species. Additionally, communities sampled at large geographic scales will have mostly separate evolutionary histories.…”

Section: Introductionmentioning

confidence: 99%

Multiple metrics of latitudinal patterns in insect pollination and herbivory for a tropical‐temperate congener pair

Baskett

Schroeder

Weber

et al. 2020

Ecological Monographs

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The biotic interactions hypothesis posits that biotic interactions are more important drivers of adaptation closer to the equator, evidenced by "stronger" contemporary interactions (e.g., greater interaction rates) and/or patterns of trait evolution consistent with a history of stronger interactions. Support for the hypothesis is mixed, but few studies span tropical and temperate regions while experimentally controlling for evolutionary history. Here, we integrate field observations and common garden experiments to quantify the relative importance of pollination and herbivory in a pair of tropical-temperate congeneric perennial herbs. Phytolacca rivinoides and P. americana are pioneer species native to the Neotropics and the eastern United States, respectively. We compared plant-pollinator and plant-herbivore interactions between three tropical populations of P. rivinoides from Costa Rica and three temperate populations of P. americana from its northern range edge in Michigan and Ohio. For some metrics of interaction importance, we also included three subtropical populations of P. americana from its southern range edge in Florida. This approach confounds species and region but allows us, uniquely, to measure complementary proxies of interaction importance across a tropicaltemperate range in one system. To test the prediction that lower-latitude plants are more reliant on insect pollinators, we quantified floral display and reward, insect visitation rates, and self-pollination ability (autogamy). To test the prediction that lower-latitude plants experience more herbivore pressure, we quantified herbivory rates, herbivore abundance, and leaf palatability. We found evidence supporting the biotic interactions hypothesis for most comparisons between P. rivinoides and north-temperate P. americana (floral display, insect visitation, autogamy, herbivory, herbivore abundance, and young-leaf palatability). Results for subtropical P. americana populations, however, were typically not intermediate between P. rivinoides and north-temperate P. americana, as would be predicted by a linear latitudinal gradient in interaction importance. Subtropical young-leaf palatability was intermediate, but subtropical mature leaves were the least palatable, and pollination-related traits did not differ between temperate and subtropical regions. These nonlinear patterns of interaction importance suggest future work to link interaction importance to climatic or biotic thresholds. In sum, we found that the biotic interactions hypothesis was more consistently supported at the larger spatial scale of our study.

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Comparative foliar metabolomics of a tropical and a temperate forest community

Cited by 51 publications

References 32 publications

Host affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistry

Host affinity of endophytic fungi and the potential for reciprocal interactions involving host secondary chemistry

The many dimensions of phytochemical diversity: linking theory to practice

Multiple metrics of latitudinal patterns in insect pollination and herbivory for a tropical‐temperate congener pair

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