Comparative flow cytometric estimation of nuclear DNA content in oil palm (Elaeis guineensis Jacq) tissue cultures and seed-derived plants

Rival, Alain; Beulé, Thierry; Barré, Philippe; Hamon, Serge; Duval, Yves; Noirot, Michel

doi:10.1007/s002990050339

Cited by 92 publications

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“…Its diploid genome consists of 16 chromosome pairs (Maria et al 1995), and the content of its nuclear DNA has been estimated by flow cytometry as 3.9 pg/2C (Rival et al 1997). There are only two species in the genus Elaeis: E. guineensis Jacq.…”

Section: Introductionmentioning

confidence: 99%

Quantitative trait loci (QTLs) analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera (H.B.K.) Cortés and oil palm (Elaeis guineensis Jacq.)

Montoya¹,

Lopes

Flori

et al. 2013

Tree Genetics & Genomes

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Section: Introductionmentioning

confidence: 99%

Quantitative trait loci (QTLs) analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera (H.B.K.) Cortés and oil palm (Elaeis guineensis Jacq.)

Montoya¹,

Lopes

Flori

et al. 2013

Tree Genetics & Genomes

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“…Its diploid genome comprises 16 pairs of homologous chromosomes (Maria et al 1995) and its nuclear DNA content has been estimated to be 2C = 3.76 pg by flow cytometry (Rival et al 1997) with a haploid genome size of 1700 Mbp. Oil palm is currently a major economic crop in Southeast Asia (particularly Malaysia and Indonesia).…”

Section: Introductionmentioning

confidence: 99%

Exploiting an oil palm EST database for the development of gene-derived SSR markers and their exploitation for assessment of genetic diversity

et al. 2008

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A total of 5,521 expressed sequence tags (ESTs) from oil palm were used to search for type and frequency of simple sequence repeat (SSR) markers. Dimeric repeat motifs appeared to be the most abundant, followed by tri-nucleotide repeats. Redundancy was eliminated in the original EST set, resulting in 145 SSRs in 136 unique ESTs (114 singletons and 22 clusters). Primers were designed for 94 (69.1%) of the unique ESTs (consisting of 14 consensus and 80 singletons). Primers for 10 EST-SSRs were developed and used to evaluate the genetic diversity of 76 accessions of oil palm originating from seven countries in Africa, and the standard Deli dura population. The average number of observed and effective alleles was 2.56 and 1.84, respectively. The EST-SSR markers were found to be polymorphic with a mean polymorphic information content value of 0.53. Genetic differentiation (FST) among the populations studied was 0.2492 indicating high level of genetic divergence. Moreover, the UPGMA (unweighted pair-group method with arithmetic mean) analysis revealed a strong association between genetic distance and geographic location of the populations studied. The germplasm materials exhibited higher diversity than Deli dura, indicating their potential usefulness in oil palm improvement programmes. The study also revealed that the populations from Nigeria, Congo and Cameroon showed the highest diversity among the germplasm evaluated in this study. The EST-SSRs further demonstrated their worth as a new source of polymorphic markers for phylogenetic analysis, since a high percentage of the markers showed transferability across species and palm taxa.

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“…The value calculated in this study was slightly different from values reported for other Arecaceae species (Bharathan et al, 1994;Rival et al, 1997). The first study of Arecaceae was carried out using Feulgen densitometry to investigate 83 species that represented 53 genera, and the 2C DNA content ranged from 1.90 pg in a diploid species (Phytelephas aequarorialis Spruce) to 78.20 pg in a species with a high polyploidy level (38x) (Voanioala gerardii J.…”

Section: Discussionmentioning

confidence: 88%

Coconut genome size determined by flow cytometry: Tall versus Dwarf types

et al. 2016

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ABSTRACT. Coconuts (Cocos nucifera L.) are tropical palm trees that are classified into Tall and Dwarf types based on height, and both types are diploid (2n = 2x = 32 chromosomes). The reproduction mode is autogamous for Dwarf types and allogamous for Tall types. One hypothesis for the origin of the Dwarf coconut suggests that it is a Tall variant that resulted from either mutation or inbreeding, and differences in genome size between the two types would support this hypothesis. In this study, we estimated the genome sizes of 14 coconut accessions (eight Tall and six Dwarf types) using flow cytometry. Nuclei were extracted from leaf discs and stained with propidium iodide, and Pisum sativum (2C = 9.07 pg DNA) was used as an internal standard. Histograms with good resolution and low coefficients of variation (2.5 to 3.2%) were obtained. The 2C DNA content ranged from 5.72 to 5.48 pg for Tall accessions and from 5.58 to 5.52 pg for Dwarf accessions. The mean genome sizes for Tall and Dwarf specimens were 5.59 and 5.55 pg, respectively. Among all accessions, Rennel Island Tall had the highest mean DNA content (5.72 pg), whereas West African Tall had the lowest (5.48 pg). The mean coconut genome size (2C = 5.57 pg, corresponding to 2723.73 Mbp/haploid set) was classified as small. Only small differences in genome size existed among the coconut accessions, suggesting that the Dwarf type did not evolve from the Tall type.

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Comparative flow cytometric estimation of nuclear DNA content in oil palm (Elaeis guineensis Jacq) tissue cultures and seed-derived plants

Cited by 92 publications

References 0 publications

Quantitative trait loci (QTLs) analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera (H.B.K.) Cortés and oil palm (Elaeis guineensis Jacq.)

Quantitative trait loci (QTLs) analysis of palm oil fatty acid composition in an interspecific pseudo-backcross from Elaeis oleifera (H.B.K.) Cortés and oil palm (Elaeis guineensis Jacq.)

Exploiting an oil palm EST database for the development of gene-derived SSR markers and their exploitation for assessment of genetic diversity

Coconut genome size determined by flow cytometry: Tall versus Dwarf types

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