Comparative ferrokinetic study with initial and extended iron clearance models.

Howarth, J. C.; Waters, H M; Hyde, K; Shanks, Dennis; Geary, C. G.

doi:10.1136/jcp.44.5.395

Cited by 5 publications

(4 citation statements)

References 13 publications

(1 reference statement)

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“…The close correlation observed between the ETU and MIT for the renal patients has also been reported for cases of myelofibrosis and myelodysplasia. 27 The advantage of ETU measurements resides in the fact that a one day study period only is required, rather than the 10 to 14 days necessary for MIT measurements. The latter method, however, has the advantage of differentiating effective from ineffective erythropoiesis.…”

Section: Discussionmentioning

confidence: 99%

Effects of azathioprine on response of renal anaemia to subcutaneous recombinant human erythropoietin.

et al. 1993

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Aims: To determine the effect of concomitant azathioprine treatment on the response of patients with renal failure to treatment with subcutaneous recombinant human erythropoietin (r-HuEPO). Methods: Two groups of patients with renal failure not receiving haemodialysis were studied. One comprised seven patients receiving erythropoietin alone, the second consisted of nine patients who were also treated with azathioprine. The haematological changes were monitored, and the functional erythropoietic response was studied by two different ferrokinetic models. One analysed the initial, the other the extended plasma iron clearance. Studies were performed

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Section: Discussionmentioning

confidence: 99%

Effects of azathioprine on response of renal anaemia to subcutaneous recombinant human erythropoietin.

et al. 1993

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“…The 14-day erythrokinetic model we have chosen was described over 15 years ago [13] but a less laborious 1-day test, which only measures the initial 3-hour clearance of 59Fe-labelled transferrin [12], is more widely used in clinical practice. The I-day model produces a parameter termed the erythron transferrin uptake (ETU) which is analogous to the M IT [15] but which fails to distinguish between effective and ineffective erythropoiesis. We have measured the ferrokinetic parameters using the 1-day model in parallel with the measurements reported here [18].…”

Section: Discussionmentioning

confidence: 99%

“…Ferrokinetic studies were carried out using the method of Ricketts [13,15]. Briefly, autologous transferrin is labelled with ?,'Fe (0.185-0.370 MBq) and autologous erythrocytes are labelled with 5lCr (1.85 MBq).…”

Section: Methodsmentioning

confidence: 99%

“…We employed a sophisticated 14-day ferrokinetic model, capable of differentiating between effective and ineffective erythropoiesis [13][14][15]. Our aims were: (a) to study the in fluence of azathioprine on erythropoiesis; (b) to investigate the effect of recombinant human erythropoietin in the presence of azathioprine; (c) to establish whether the azathioprine-treated could safely enjoy the same benefits from erythropoietin therapy as other patients with renal failure.…”

Section: Introductionmentioning

confidence: 99%

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Influence of Azathioprine on the Ferrokinetics of Patients with Renal Failure before and after Treatment with Erythropoietin

Anastassiades

Howarth²,

Howarth³

et al. 1994

Nephron

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In 16 patients (9 on azathioprine, 7 not) the ineffective iron turnover (I IT) was much higher in the azathioprine group (62.7 ± 6.7 vs. 23.5 ± 3.5 μmol/l blood/ day, p < 0.0001, 2-tailed t test), though the red cell iron turnover (ROT) was similar (42.8 ± 2.9 vs. 41 ± 4.8). Erythropoietin improved the anaemia in all patients and raised the RCIT (4 still on azathioprine to 72.2 ± 9.8, p < 0.003; 7 non-azathioprine patients to 62.7 ± 5.3, p < 0.01); the ΠT remained higher in the azathioprine-treated (85.5 ± 19.3 vs. 37.1 ± 5.4; p < 0.013). In 2 patients who discontinued azathioprine, the IIT declined markedly to normal. In summary, azathioprine exacerbates the anaemia of renal failure by augmenting ineffective erythropoiesis, while erythropoietin benefits those on azathioprine as much as other renal patients by stimulating both effective and ineffective erythropoiesis.

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Erythropoiesis in primary (idiopathic) osteomyelofibrosis: Quantification, PCNA‐reactivity, and prognostic impact

Thiele¹,

Windecker²,

Kvasnicka³

et al. 1994

American J Hematol

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In 64 patients with primary (idiopathic) osteomyelofibrosis (OMF), a morphometric analysis has been performed on bone marrow trephine biopsies following sequential double-immunostaining with monoclonal antibodies against proliferating cell nuclear antigen (PCNA) and erythroid precursor cells (glycophorin C). The purpose of this study was to quantify erythropoiesis and its PCNA-staining capacity and, further, to determine the impact of these parameters for the development of anemia and for prognosis. In comparison with a control group (15 patients), a significant reduction in the number of erythro-normoblasts could be demonstrated, associated with an increase in PCNA-labelling. Moreover, significant correlations between the amount of nucleated erythroid marrow cells and degree of anemia (hemoglobin level, hematocrit, erythrocyte count) and survival could be calculated. Adverse relationships were assessed between number of erythroid cells, thrombocyte count, and spleen size, and also argyrophilic (reticulin/collagen) fiber density. These interactions were thought to reflect the biological behaviour of the disease process, i.e., the progression or extent of myeloid metaplasia. Our findings support ferrokinetic studies suggesting erythroid hypoplasia as one of the major causes of anemia in OMF. The remarkable high PCNA-labelling index of the macrocytic-megaloblastoid appearing erythropoiesis is probably caused by an overexpression of this marker protein. A comparative evaluation of Ki-67 antigen immunostaining in splenic tissue (myeloid metaplasia) and of the PCNA-labelling in pernicious anemia lend support to the assumption of an undue prolongation of the S-phase generated by secondary folate (hematinic) deficiency.

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Comparative ferrokinetic study with initial and extended iron clearance models.

Cited by 5 publications

References 13 publications

Effects of azathioprine on response of renal anaemia to subcutaneous recombinant human erythropoietin.

Effects of azathioprine on response of renal anaemia to subcutaneous recombinant human erythropoietin.

Influence of Azathioprine on the Ferrokinetics of Patients with Renal Failure before and after Treatment with Erythropoietin

Erythropoiesis in primary (idiopathic) osteomyelofibrosis: Quantification, PCNA‐reactivity, and prognostic impact

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