Comparative expression pathway analysis of human and canine mammary tumors

Uva, Paolo; Aurisicchio, Luigi; Watters, James; Loboda, Andrey; Kulkarni, Amit; Castle, John C.; Palombo, Fabio; Viti, Valentina; Mesiti, Giuseppe; Zappulli, Valentina; Marconato, Laura; Abramo, Francesca; Ciliberto, Gennaro; Lahm, Armin; Monica, Nicola La; Rinaldis, Emanuele de

doi:10.1186/1471-2164-10-135

Cited by 156 publications

(148 citation statements)

References 89 publications

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“…A etiologia do câncer de mama é multifatorial, envolvendo a participação de componentes genéticos, hormonais, nutricionais e ambientais (Henderson & Feigelson, 2000;Silva et al, 2004;Uva et al, 2009;Andrade et al, 2010). As alterações no estilo de vida da sociedade moderna contribuem com uma maior exposição da população humana a alguns fatores potencialmente carcinogê-nicos, havendo interferência também nos hábitos alimentares dos animais, os quais passam a estar sob riscos semelhantes (Silva et al, 2004;Dagli, 2008).…”

Section: Introductionunclassified

Caracterização clínica, diagnóstico histopatológico e distribuição geográfica das neoplasias mamárias em cadelas de Salvador, Bahia

Toríbio

Filho²,

Ribeiro³

et al. 2012

Rev. Ceres

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Clinical characterization, histopathologic diagnosis and geoprocessing of mammary tumours in bitches from the city of Salvador, Bahia StateSpontaneous tumors of the mammary gland in female dogs, represent the most frequent neoplasms observed in the small animal clinic, which explains the high interest of researchers in the study of this lesion. This study aimed to determine the frequency of mammary tumors in female dogs treated at HOSPMEV/UFBA, in Salvador, from January 2006 to April 2008, as well as to carry out the clinical characterization, histopathologic classification and the georeferencing of this pathology. The results showed a high prevalence of mammary tumors in female dogs, with most of the tumors in more advanced stages and the carcinomas in mixed tumors the predominant histologic type. There was Os tumores espontâneos da glândula mamária representam, nas cadelas, a neoplasia mais observada na clínica de pequenos animais, justificando o grande interesse dos pesquisadores no estudo dessa lesão. Este trabalho teve por objetivo determinar a frequência dos tumores mamários em cadelas atendidas no HOSPMEV/UFBA, no município de Salvador, no período de janeiro de 2006 a abril de 2008, bem como realizar a caracterização clínica, a classificação histopatológica e o georreferenciamento dessa patologia. Os resultados revelaram alta prevalência de neoplasias mamárias em cadelas, em sua maioria tumores, em estádios mais avançados, sendo os carcinomas em tumores mistos o tipo histológico predominante. Observou-se ainda a correlação das características socioeconômicas da população estudada com fatores sabidamente prognósticos para neoplasias mamárias em cadelas. Esses resultados refletem a procura tardia da assistência Médica Veterinária. Por isso, é urgente educar a população quanto à prevenção e detecção da neoplasia mamária nas cadelas, buscando o diagnóstico precoce e a terapêutica adequada.Palavras-chave: tumor de mama, estadiamento clínico, fatores socioeconômicos.

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Section: Introductionunclassified

Caracterização clínica, diagnóstico histopatológico e distribuição geográfica das neoplasias mamárias em cadelas de Salvador, Bahia

Toríbio

Filho²,

Ribeiro³

et al. 2012

Rev. Ceres

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“…Additional and reliable tools for effective therapy are required in veterinary medicine. Furthermore, a recent study revealed clear evidence of a similarity between human and dog tumors in regard to the deregulation of several cancer-related genes, including PI3K/Akt, PTEN and Wnt-β catenin and MAPK signaling (5). Accordingly, canine mammary tumors can also provide a suitable natural model for the comparative study of human breast cancer (5)(6)(7).…”

Section: Introductionmentioning

confidence: 99%

4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells

et al. 2012

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Abstract. Hyaluronan (HA), a major component of the extracellular matrix (ECM), is synthesized by HA synthase (HAS) 1, HAS2 and HAS3 and is intricately involved in cell growth and metastasis. The HA synthesis inhibitor 4-methylumbelliferone (4-MU) has been reported to exhibit anticancer properties in various types of malignant tumors. However, the underlying mechanisms at the molecular and cellular levels remain unclear. In this study, to establish an animal model for studying the function of HA in human breast cancer, we investigated the antitumor effects of 4-MU using canine mammary tumor (CF33) cells. First, we investigated the effects of 4-MU on HA production in CF33 cells. Quantitative analysis of HA in culture media showed that 4-MU inhibited HA synthesis, accompanied by downregulation of HAS2 mRNA levels, in a dose-dependent manner at 24-72 h. Additionally, we observed a 4-MU-mediated decrease in the extent of the cell-associated HA matrix. We examined the effect of 4-MU on cell growth and apoptosis in CF33 cells. 4-MU markedly inhibited cell proliferation and induced apoptosis in CF33 cells. In particular, our experiments showed that the mechanism of 4-MU-induced apoptosis in CF33 cells involved increased levels of expression of pro-apoptotic BAX mRNA and protein molecules. These data suggest that 4-MU may be a candidate therapeutic agent for the treatment of canine mammary tumors. Furthermore, this study provides the first indication that the canine mammary tumor may be a suitable model for comparative study of the function of HA in human breast cancer. IntroductionMammary tumors in dogs occur at a frequency of 3 times the incidence of mammary tumors in humans and have recorded a higher incidence rate than other livestock (1). Mammary tumors are the most frequent cutaneous neoplasms in female dogs, and approximately 50% are diagnosed as malignant tumors (2). Following surgical excision, approximately 48% of the affected dogs die or are euthanized within 1 year due to tumor recurrence or metastasis (3). Canine mammary tumors are heterogeneous, and the different clinical and biological features (4) make it difficult to determine a prognosis and treatment for affected dogs, as is often the case in humans. Additional and reliable tools for effective therapy are required in veterinary medicine. Furthermore, a recent study revealed clear evidence of a similarity between human and dog tumors in regard to the deregulation of several cancer-related genes, including PI3K/Akt, PTEN and Wnt-β catenin and MAPK signaling (5). Accordingly, canine mammary tumors can also provide a suitable natural model for the comparative study of human breast cancer (5-7).Hyaluronan (HA) is a non-sulfated linear glycosaminoglycan that consists of repeating disaccharide subunits of glucuronic acid and N-acetylglucosamine (8,9). It is ubiquitously distributed in the extra-and pericellular spaces of most animal tissues (8,9). HA plays a critical role in regulating matrix assembly, cell migration, differentiation and proliferation (9-11)...

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“…However, comparative studies of these carcinomas during the first developmental stages have received less attention [10,73,74]. The detection of early lesions in MG has demonstrated its importance for preventing and diminishing the mor-that within the same MG there is substantial heterogeneity and that the benign and malignant lesions are multifocal and have variable size, they did not interpret the meaning of these findings.…”

Section: Discussionmentioning

confidence: 85%

Histologic Evidence of the "Sick Lobe" Carcinogenesis Theory and the Frequency of Intraepithelial Lesions in the Mammary Glands of Female Canines with no Clinical Signs of Neoplasia

Caicedo¹,

Iregui²

2017

Int J Pathol Clin Res

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Comparative expression pathway analysis of human and canine mammary tumors

Cited by 156 publications

References 89 publications

Caracterização clínica, diagnóstico histopatológico e distribuição geográfica das neoplasias mamárias em cadelas de Salvador, Bahia

Caracterização clínica, diagnóstico histopatológico e distribuição geográfica das neoplasias mamárias em cadelas de Salvador, Bahia

4-Methylumbelliferone leads to growth arrest and apoptosis in canine mammary tumor cells

Histologic Evidence of the "Sick Lobe" Carcinogenesis Theory and the Frequency of Intraepithelial Lesions in the Mammary Glands of Female Canines with no Clinical Signs of Neoplasia

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