Comparative Expression of Key Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Tubers of Two Potato Cultivars, Atlantic and Haryoung

Mekapogu, Manjulatha; Sohn, Hwang Bae; Kim, Yul Ho; Kim, Su Jeong; Cho, Kwang Soo; Kwon, Oh Keun; Jin, Yong Ik; Hong, Su Young; Nam, Jeong Hwan; Suh, Jong Taek; Choi, Ji Kyoung; Jeong, Ji Won

doi:10.9787/pbb.2014.2.3.257

Cited by 4 publications

(2 citation statements)

References 37 publications

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“…The expression levels of Hmg1, Hmg2, Pss1, and Sgt2 in the five genotypes were significantly lower than the expression levels of Pvs1, Sgt1, and Sgt3. This is consistent with the findings of Manjulatha et al [29], who reported that the expression levels of Hmg1 and Pss1 can be used as selection markers for breeding potato cultivars with low levels of SGAs.…”

Section: Discussionsupporting

confidence: 93%

GGE Biplot-Based Transcriptional Analysis of 7 Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Potato (Solanum tuberosum L.)

Zhang

Cui

et al. 2023

Agronomy

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Steroidal glycoalkaloids (SGAs) are secondary metabolites that are closely associated with the sensory and processing qualities of potato tubers. GGE biplots are a widely used tool for analyzing crop breeding analysis. This study aimed to investigate the effect of light on SGA biosynthesis by employing GGE biplots to analyze the transcriptional gene expression of seven genes involved in the SGA biosynthesis pathway. Tubers of five different potato genotypes were incubated for 6, 12, and 24 h under red light. The expression levels of the seven genes were measured using qRT-PCR for analysis. Further analysis of the data was performed using GGE biplots. Our results indicated significantly higher expression levels for Pvs1, Sgt1, and Sgt3 genes than those of the remaining tested genes. Across the three red light illumination durations, Sgt3 showed high and stable expression, although it showed less stability across the different genotypes. Interestingly, the expression patterns of the seven genes were extremely similar for the 12 h and 24 h treatments. It was found that at least 6 h of red light illumination was required for optimal gene expression in all five genotypes, particularly in the genotype Zhuangshu-3 (DXY) after 24 h of treatment. Additionally, significant expression of the seven genes was observed in the L-6 genotype after 12 and 6 h of red light illumination. These results highlight that GGE biplots are an appropriate tool for analyzing and illustrating the differential expression profiles of the seven key genes involved in SGA biosynthesis in potato tubers. This study provides valuable insights into the biosynthesis and metabolism of SGAs in potatoes. Moreover, it demonstrates the potential application of GGE biplots in crop breeding and other research fields.

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Section: Discussionsupporting

confidence: 93%

GGE Biplot-Based Transcriptional Analysis of 7 Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Potato (Solanum tuberosum L.)

Zhang

Cui

et al. 2023

Agronomy

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“…Atlantic) and high SGAs content (cv. Haryoung), showed a very different transcript accumulation of HMG1 and PSS1 genes, under drought stress conditions, being doubled in the cultivar Haryoung [ 114 ]. The mRNA level of these two genes could be used as selection markers for breeding potatoes with low SGAs level.…”

Section: Biocompounds In Potato Peelmentioning

confidence: 99%

Sustainable Use of Bioactive Compounds from Solanum Tuberosum and Brassicaceae Wastes and by-Products for Crop Protection—A Review

et al. 2021

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Defatted seed meals of oleaginous Brassicaceae, such as Eruca sativa, and potato peel are excellent plant matrices to recover potentially useful biomolecules from industrial processes in a circular strategy perspective aiming at crop protection. These biomolecules, mainly glycoalkaloids and phenols for potato and glucosinolates for Brassicaceae, have been proven to be effective against microbes, fungi, nematodes, insects, and even parasitic plants. Their role in plant protection is overviewed, together with the molecular basis of their synthesis in plant, and the description of their mechanisms of action. Possible genetic and biotechnological strategies are presented to increase their content in plants. Genetic mapping and identification of closely linked molecular markers are useful to identify the loci/genes responsible for their accumulation and transfer them to elite cultivars in breeding programs. Biotechnological approaches can be used to modify their allelic sequence and enhance the accumulation of the bioactive compounds. How the global challenges, such as reducing agri-food waste and increasing sustainability and food safety, could be addressed through bioprotector applications are discussed here.

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Medicinal metabolites with common biosynthetic pathways in Solanum nigrum

Jabamalairaj

Priatama

Heo

et al. 2019

Plant Biotechnol Rep

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Comparative Expression of Key Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Tubers of Two Potato Cultivars, Atlantic and Haryoung

Cited by 4 publications

References 37 publications

GGE Biplot-Based Transcriptional Analysis of 7 Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Potato (Solanum tuberosum L.)

GGE Biplot-Based Transcriptional Analysis of 7 Genes Involved in Steroidal Glycoalkaloid Biosynthesis in Potato (Solanum tuberosum L.)

Sustainable Use of Bioactive Compounds from Solanum Tuberosum and Brassicaceae Wastes and by-Products for Crop Protection—A Review

Medicinal metabolites with common biosynthetic pathways in Solanum nigrum

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