Comparative expression of genes controlling cell wall-degrading enzymes in Verticillium dahliae isolates from olive, potato and sunflower

Gharbi, Yâakoub; Alkher, H.; Triki, Mohamed Ali; Barkallah, Maher; Emna, B.; Trabelsi, Rahma; Fendri, Imen; Gdoura, Radhouane; Daayf, Fouad

doi:10.1016/j.pmpp.2015.05.006

Cited by 17 publications

(11 citation statements)

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“…These results provided a proof of the involvement of cell walldegrading genes in the initial steps of the roots infections and likely in pathogenesis. Recently, functional studies of cell wall-degrading related genes by targeted gene knockout have been carried out to obtain mutants deficient in one or more these genes [60,67], but were not conclusive due to the multigene families encoding these enzymes [68]. Therefore, it is important to detect which genes were responsible for the pathogenicity of V. dahliae.…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

et al. 2020

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Background: Cotton Verticillium wilt is one of the most devastating diseases for cotton production in the world. Although this diseases have been widely studied at the molecular level from pathogens, the molecular basis of V. dahliae interacted with cotton has not been well examined. Results: In this study, RNA-seq analysis was carried out on V. dahliae samples cultured by different root exudates from three cotton cultivars (a susceptible upland cotton cultivar, a tolerant upland cotton cultivar and a resistant island cotton cultivar) and water for 0 h, 6 h, 12 h, 24 h and 48 h. Statistical analysis of differentially expressed genes revealed that V. dahliae responded to all kinds of root exudates but more strongly to susceptible cultivar than to tolerant and resistant cultivars. Go analysis indicated that 'hydrolase activity, hydrolyzing O-glycosyl compounds' related genes were highly enriched in V. dahliae cultured by root exudates from susceptible cotton at early stage of interaction, suggesting genes related to this term were closely related to the pathogenicity of V. dahliae. Additionally, 'transmembrane transport', 'coenzyme binding', 'NADP binding', 'cofactor binding', 'oxidoreductase activity', 'flavin adenine dinucleotide binding', 'extracellular region' were commonly enriched in V. dahliae cultured by all kinds of root exudates at early stage of interaction (6 h and 12 h), suggesting that genes related to these terms were required for the initial steps of the roots infections. Conclusions: Based on the GO analysis results, the early stage of interaction (6 h and 12 h) were considered as the critical stage of V. dahliae-cotton interaction. Comparative transcriptomic analysis detected that 31 candidate genes response to root exudates from cotton cultivars with different level of V. dahliae resistance, 68 response to only susceptible cotton cultivar, and 26 genes required for development of V. dahliae. Collectively, these expression data have advanced our understanding of key molecular events in the V. dahliae interacted with cotton, and provided a framework for further functional studies of candidate genes to develop better control strategies for the cotton wilt disease.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

et al. 2020

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“…In addition to serving as a preformed physical barrier, the cell wall responds dynamically to biotic stress by the formation of lignin and suberin, depositions of callose and cross-linking and remodelling of the cell wall structure (Bellincampi et al 2014 ). It undergoes significant modifications also due to pathogen released cell wall-degrading enzymes, which have been clearly associated with pathogenicity of V. dahliae (Gharbi et al 2015 ). Cutin metabolism was the most significantly enriched GO process in resistant hop.…”

Section: Discussionmentioning

confidence: 99%

Comparative transcriptional analysis of hop responses to infection with Verticillium nonalfalfae

et al. 2017

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Key Message Dynamic transcriptome profiling revealed excessive, yet ineffective, immune response to V. nonalfalfae infection in susceptible hop, global gene downregulation in shoots of resistant hop and only a few infection-associated genes in roots. AbstractHop (Humulus lupulus L.) production is hampered by Verticillium wilt, a disease predominantly caused by the soil-borne fungus Verticillium nonalfalfae. Only a few hop cultivars exhibit resistance towards it and mechanisms of this resistance have not been discovered. In this study, we compared global transcriptional responses in roots and shoots of resistant and susceptible hop plants infected by a lethal strain of V. nonalfalfae. Time-series differential gene expression profiles between infected and mock inoculated plants were determined and subjected to network-based analysis of functional enrichment. In the resistant hop cultivar, a remarkably low number of genes were differentially expressed in roots in response to V. nonalfalfae infection, while the majority of differentially expressed genes were down-regulated in shoots. The most significantly affected genes were related to cutin biosynthesis, cell wall biogenesis, lateral root development and terpenoid biosynthesis. On the other hand, susceptible hop exhibited a strong defence response in shoots and roots, including increased expression of genes associated with plant responses, such as innate immunity, wounding, jasmonic acid pathway and chitinase activity. Strong induction of defence-associated genes in susceptible hop and a low number of infection-responsive genes in the roots of resistant hop are consistent with previous findings, confirming the pattern of excessive response of the susceptible cultivar, which ultimately fails to protect the plant from V. nonalfalfae. This research offers a multifaceted overview of transcriptional responses of susceptible and resistant hop cultivars to V. nonalfalfae infection and represents a valuable resource in the study of this plant-pathogen interaction.Electronic supplementary materialThe online version of this article (doi:10.1007/s00299-017-2177-1) contains supplementary material, which is available to authorized users.

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“…However, many insect pests and plant pathogens constantly threaten olive cultivation. The dieback and wilting symptoms induced by complex soilborne fungi has caused considerable economic losses in olive orchards in Tunisia (Boulila and Mahjoub 1994; Triki et al 2006, 2009, 2011; Gharbi et al 2014, 2015). Among these fungi, Fusarium species may cause disease in olive trees.…”

Section: Introductionmentioning

confidence: 99%

Morphological and molecular characterization of Fusarium spp. associated with olive trees dieback in Tunisia

et al. 2017

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Dieback and wilting symptoms caused by complex soilborne fungi are nowadays the most serious threatening disease affecting olive trees (Olea europaea) in Tunisia and presumably in many Mediterranean basin countries. Fusarium is one of the important phytopathogenic genera associated with dieback symptoms of olive trees. The objective of the present study was to confirm the pathogenicity of Fusarium spp. isolated from several olive-growing areas in Tunisia. According to the pathogenic test done on young olive trees (cv. Chemlali), 23 out of 104 isolates of Fusarium spp. were found to be pathogenic and the others were weakly or not pathogenic. The pathogenic Fusarium spp. isolates were characterized using molecular methods based on ITS PCR. Isolation results revealed the predominance of Fusarium solani (56.5%) and F. oxysporum species (21.7%) compared to F. chalmydosporum (8.7%), F. brachygibbosum (8.7%) and F. acuminatum (4.34%). Based on pathogenicity test, disease severity was highly variable among the 23 pathogenic isolates tested (P < 0.05) where F. solani was the most aggressive dieback agent. To the best of our knowledge, this is the first work that shows that Fusarium spp. might be a major agent causing dieback disease of olive trees in Tunisia.

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Comparative expression of genes controlling cell wall-degrading enzymes in Verticillium dahliae isolates from olive, potato and sunflower

Cited by 17 publications

References 55 publications

Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

Transcriptomic analysis of gene expression of Verticillium dahliae upon treatment of the cotton root exudates

Comparative transcriptional analysis of hop responses to infection with Verticillium nonalfalfae

Morphological and molecular characterization of Fusarium spp. associated with olive trees dieback in Tunisia

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