Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus

Martínez‐Alcántara, Belén; Martínez-Cuenca, Mary-Rus; Quiñones, Ana; Iglesias, Domingo J.; Primo‐Millo, Eduardo; Forner-Giner, María Ángeles

doi:10.1016/j.envexpbot.2014.11.002

Cited by 28 publications

(18 citation statements)

References 93 publications

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“…These results indicate that ginseng cells tend to pump excess sodium ions out of cells, rather than store them in cell compartments. A similar tendency was recently reported in Populus (Martínez-Alcántara et al, 2015 ), but the reverse was reported for citrus (Peng et al, 2016 ) and upland cotton (Zhang et al, 2004 ). This pumping process was coupled with the electrochemical gradient generated by H + -ATPases.…”

Section: Discussionsupporting

confidence: 85%

A Growth-Promoting Bacteria, Paenibacillus yonginensis DCY84T Enhanced Salt Stress Tolerance by Activating Defense-Related Systems in Panax ginseng

et al. 2018

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Panax ginseng (C.A. Mayer) is a well-known medicinal plant used in traditional medicine in Korea that experiences serious salinity stress related to weather changes or incorrect fertilizer application. In ginseng, the use of Paenibacillus yonginensis DCY84T to improve salt stress tolerance has not been thoroughly explored. Therefore, we studied the role of P. yonginensis DCY84T under short-term and long-term salinity stress conditions in a controlled environment. In vitro testing of DCY84T revealed high indole acetic acid (IAA) production, siderophore formation, phosphate solubilization and anti-bacterial activity. We determined that 10-min dip in 1010 CFU/ml DCY84T was sufficient to protect ginseng against short-term salinity stress (osmotic stress) upon exposure to 300 mM NaCl treatment by enhancing nutrient availability, synthesizing hydrolyzing enzymes and inducing osmolyte production. Upon exposure to salinity stress (oxidative and ionic stress), strain DCY84T-primed ginseng seedlings were protected by the induction of defense-related systems such as ion transport, ROS scavenging enzymes, proline content, total sugars, and ABA biosynthetic genes, as well as genes involved in root hair formation. Additionally, ginseng primed with DCY84T and exposed to 300 mM NaCl showed the same metabolite profile as control ginseng plants, suggesting that DCY84T effectively reduced salt stress. These results indicated that DCY84T can be widely used as a microbial inoculant to protect ginseng plants against salinity stress conditions.

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Section: Discussionsupporting

confidence: 85%

A Growth-Promoting Bacteria, Paenibacillus yonginensis DCY84T Enhanced Salt Stress Tolerance by Activating Defense-Related Systems in Panax ginseng

et al. 2018

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“…A raised expression level of V-PPiase in +B organs, in comparison to those of Ct plants, suggests that V-PPiase is likely involved in the acidification of the vacuole while no effect was recorded on V-ATPase A. The protonmotive force generated by this vacuolar H + -translocating enzyme, enhances antiporters involved in B transport to increase their activity and, consequently, B sequestration into this organelle is more efficient, similarly to what is described for sodium tonoplast antiporters during salt stress conditions [ 47 , 48 ]. Maybe even the action of putative tonoplast antiporters of polyols (due to their ability to complex with B) might play here as secondary transporters energized by the tonoplast H + gradient and its role should not be discarded.…”

Section: Resultsmentioning

confidence: 84%

Physiological and Molecular Responses to Excess Boron in Citrus macrophylla W

et al. 2015

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This work provides insight into several mechanisms involved in boron (B) regulation pathway in response to high B conditions in Citrus. The study was carried out in Citrus macrophylla W. (Cm) seedlings cultured “in vitro” in media with 50 or 400 μM H3BO3 (control, Ct, and B-excess, +B, plants, respectively). Growth parameters, B concentration, leaf chlorophyll (Chl) concentration, the expression of the main putative genes involved in B transport and distribution, and leaf and root proline and malonaldehyde (MDA) concentrations, were assessed. Excess B led to high B concentration in +B plants (3.8- and 1.4-fold in leaves and roots, respectively) when compared with Ct ones. However, a minor effect was recorded in the plant (incipient visual symptoms, less than 27% reduction in root growth and 26% decrease in Chl b concentration). B toxicity down-regulated by half the expression level of putative B transporter genes NIP5 and PIP1. CmBOR1 gene was not repressed in +B plants and B accumulated in the shoots. High B level increased the transcripts of putative gene TIP5, involved in B transport across the tonoplast, by 3.3- and 2.4-fold in leaves and roots, respectively. The activity of V-PPiase proton pump, related with the electrochemical gradient in the vacuole, was also enhanced in +B organs. B toxicity up-regulated putative BOR4 gene (2.1- and 2.7-fold in roots and leaves, respectively), which codifies for an active efflux B transporter. Accordingly, B was located in +B plants preferently in an insoluble form on cell walls. Finally, excess B caused a significant rise in proline concentration (51% and 34% in roots and leaves, respectively), while the MDA level did not exceed 20%. In conclusion, Cm tolerance to a high B level is likely based on the synergism of several specific mechanisms against B toxicity, including: 1/ down-regulation of NIP5 and PIP1 boron transporters; 2/ activation of B efflux from cells due to the up-regulation of putative BOR4 gene; 3/ compartmentation of B in the vacuole through TIP5 transporter activation and the acidification of the organelle; 4/ insolubilisation of B and deposition in cell walls preventing from cytoplasm damage; and, 5/ induction of an efficient antioxidant system through proline accumulation.

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“…Major differences in salt stress tolerance have been found between species and family members. Uptake and/or transport of saline ions to the scion is controlled by the rootstock, which chiefly determines chloride (Cl − ) and sodium (Na + ) accumulation in leaves [6,[9][10][11]. Since the main ion that causes damage is Cl − [12,13], the salt tolerance of some citrus rootstocks is usually established by their capacity to exclude Cl − from leaves [5].…”

Section: Salinitymentioning

confidence: 99%

Influence of Rootstock on Citrus Tree Growth: Effects on Photosynthesis and Carbohydrate Distribution, Plant Size, Yield, Fruit Quality, and Dwarfing Genotypes

Martínez-Cuenca¹,

Primo-Capella²,

Forner-Giner³

2016

Plant Growth

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Citrus species are the most widely produced fruit crops in the world, and Spain is one of the leading citrus producers that supply the fresh market. Rootstocks greatly influence variety behaviour as it ensures tolerance to abiotic stress conditions, as well as the provision of minerals and water for the total plant, and consequently impact crop yield and fruit quality. So, rootstock choice is one of the most important decisions a grower makes when establishing commercial citrus orchards. In this chapter, we attempted to provide an overview of the response in terms of plant growth, fruit quality and yield parameters of several citrus cultivar trees grafted onto different commercial rootstocks, plus new hybrids and some dwarfing genotypes, to reduce costs in some cultural practices. In particular, we considered the rootstock influence on scion photosynthetic capacity linked to carbohydrate distribution for plant vegetative and reproductive development.

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Comparative expression of candidate genes involved in sodium transport and compartmentation in citrus

Cited by 28 publications

References 93 publications

A Growth-Promoting Bacteria, Paenibacillus yonginensis DCY84T Enhanced Salt Stress Tolerance by Activating Defense-Related Systems in Panax ginseng

A Growth-Promoting Bacteria, Paenibacillus yonginensis DCY84T Enhanced Salt Stress Tolerance by Activating Defense-Related Systems in Panax ginseng

Physiological and Molecular Responses to Excess Boron in Citrus macrophylla W

Influence of Rootstock on Citrus Tree Growth: Effects on Photosynthesis and Carbohydrate Distribution, Plant Size, Yield, Fruit Quality, and Dwarfing Genotypes

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