Comparative Expression Analysis of Putative Cancer Stem Cell Markers CD44 and ALDH1A1 in Various Skin Cancer Subtypes

Erfani, Elham; Roudi, Raheleh; Rakhshan, Azadeh; Sabet, Mehrdad Nasrollahzadeh; Shariftabrizi, Ahmad; Madjd, Zahra

doi:10.5301/jbm.5000165

Cited by 38 publications

(31 citation statements)

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“…Breast CSCs are characterized by the markers CD44 +/hi /CD24 −/low (Al-Hajj et al., 2003, Blick et al., 2010, Ricardo et al., 2011) and by expression of genes that promote epithelial-mesenchymal transition (EMT) (Blick et al., 2010, Mani et al., 2008), which is critical for cancer progression and metastasis (Choi et al., 2013, Sarrio et al., 2008, Sheridan et al., 2006, Thiery, 2002, Tsai and Yang, 2013). Aggressive cancers of other tissues of origin such as thyroid, colorectum, pancreas, and skin also demonstrate expansion of the CD44 +/hi CSC population (Dou et al., 2007, Erfani et al., 2016, Jing et al., 2015, Liu and Brown, 2010, Parmiani, 2016). In contrast to the majority of cells in a tumor, CSCs/TICs have the ability to form tumor xenografts (Al-Hajj et al., 2003, Iqbal et al., 2013).…”

Section: Introductionmentioning

confidence: 99%

Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas

et al. 2016

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SummaryMany solid cancers have an expanded CD44+/hi/CD24−/low cancer stem cell (CSC) population, which are relatively chemoresistant and drive recurrence and metastasis. Achieving a more durable response requires the development of therapies that specifically target CSCs. Recent evidence indicated that inhibiting the SUMO pathway repressed tumor growth and invasiveness, although the mechanism has yet to be clarified. Here, we demonstrate that inhibition of the SUMO pathway repressed MMP14 and CD44 with a concomitant reduction in cell invasiveness and functional loss of CSCs in basal breast cancer. Similar effects were demonstrated with a panel of E1 and E3 SUMO inhibitors. Identical results were obtained in a colorectal cancer cell line and primary colon cancer cells. In both breast and colon cancer, SUMO-unconjugated TFAP2A mediated the effects of SUMO inhibition. These data support the development of SUMO inhibitors as an approach to specifically target the CSC population in breast and colorectal cancer.

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Section: Introductionmentioning

confidence: 99%

Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas

et al. 2016

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“…In this study, we have selected epidermis, specifically, for several reasons: (i) CD44 is the cell surface receptor for HA and HA is the major component of the epidermal intercellular matrix involved in the nutrition of this non-vascularized tissue, in regulation of the gradients of ions and growth factors, and in promotion of keratinocyte responses to wound healing stimuli [ 40 ]. (ii) CD44 isoforms are suggested to be involved in epidermal tumorigenesis and stem cell regulation [ 41 – 43 ]. (iii) We are currently studying the exact nature and expression of a novel keratinocyte antigen, desmosealin, which we believe being related to the CD44 family of proteoglycans [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

et al. 2016

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CD44, a cell surface proteoglycan, is involved in many biological events. CD44 transcripts undergo complex alternative splicing, resulting in many functionally distinct isoforms. To date, however, the nature of these isoforms in human epidermis has not been adequately determined. In this study, we isolated all CD44 transcripts from normal human epidermis, and studied how their expressions are regulated. By RT-PCR, we found that a number of different CD44 transcripts were expressed in human epidermis, and we obtained all these transcripts from DNA bands in agarose and acrylamide gels by cloning. Detailed sequence analysis revealed 18 CD44 transcripts, 3 of which were novel. Next, we examined effects of 10 different agents on the expression of CD44 transcripts in cultured human keratinocytes, and found that several agents, particularly epidermal growth factor, hydrogen peroxide, phorbol 12-myristate 13-acetate, retinoic acid, calcium and fetal calf serum differently regulated their expressions in various patterns. Furthermore, normal and malignant keratinocytes were found to produce different CD44 transcripts upon serum stimulation and subsequent starvation, suggesting that specific CD44 isoforms are involved in tumorigenesis via different CD44-mediated biological pathways.

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“…(ii) CD44 isoforms are suggested to be involved in epidermal tumorigenesis and stem cell regulation [41][42][43]. (iii) We are currently studying the exact nature and expression of a novel keratinocyte antigen, desmosealin, which we believe being related to the CD44 family of proteoglycans [44].…”

Section: Discussionmentioning

confidence: 99%

Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

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et al. 2016

Journal of Dermatological Science

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Comparative Expression Analysis of Putative Cancer Stem Cell Markers CD44 and ALDH1A1 in Various Skin Cancer Subtypes

Abstract: These findings suggest that a CD44high/ALDH1A1high phenotype in melanoma and a CD44high phenotype in SCC can be considered candidates for targeted therapy of skin cancers aiming at CSCs.

Cited by 38 publications

References 50 publications

Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas

Inhibiting the SUMO Pathway Represses the Cancer Stem Cell Population in Breast and Colorectal Carcinomas

Isolation of All CD44 Transcripts in Human Epidermis and Regulation of Their Expression by Various Agents

Isolation of all CD44 transcripts in human epidermis and regulation of their expression by various agents

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